Abstract: A high speed fluorescent emission imaging digital microscope has an Argon-Ion UV laser illumination source and a high speed optical filtering wheel in the illumination path to alternately select between two UV illumination wavelengths to produce fluorescent emission images at each UV wavelength. A dichroic reflector directs emission images along an image path and an galvanometer rotatable mirror exposes each image on a predetermined area of a CCD. A computer controls the rotatable mirror in response to exposure timing information from the filtering wheel. An objective lens is focused on the specimen for producing an image from a particular focal plane of the specimen. The objective lens is mounted on a counterweighted piezoelectric objective translator for changing the focal plane of the specimen image in response to control signals from the electronic processor.

Abstract: A microscope is modified to allow for precise imaging of samples using different filters at different focal planes under computer control. A filter accessory includes a filter disc having bandpass filters. The filter disc may be rotated to move a selected filter into the optical path between a light source and the microscope sample. Adjustment of the image plane of the microscope is controlled by the computer using feedback from a lens position sensor.

Abstract: A microscope is modified to allow for precise imaging of samples using different filters at different focal planes under computer control. A filter accessory includes a filter disc having bandpass filters. The filter disc may be rotated to move a selected filter into the optical path between a light source and the microscope sample. Adjustment of the image plane of the microscope is controlled by the computer using feedback from a lens position sensor.

Abstract: A microscope is modified to allow for precise imaging of samples using different filters at different focal planes under computer control. A filter accessory includes a filter disc having bandpass filters. The filter disc may be rotated to move a selected filter into the optical path between a light source and the microscope sample. Adjustment of the image plane of the microscope is controlled by the computer using feedback from a lens position sensor.

Abstract: An accessory is provided to convert a conventional microscope to a microspectrofluorimeter. The accessory includes a filter disc having bandpass filters separated by opaque segments. The filter disc is rapidly and continuously rotated to move the filter sequentially into the optical path between a wideband light source and a microscope sample. Photon counting of radiation from the sample is synchronized to the position of the filters during rotation by means of synchronization marks on the filter disc. The same accessory may be positioned between the sample and a detector to detect emitted light of different frequencies.