Patents by Inventor Da REN
Da REN has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11993663Abstract: The present invention concerns a method for preparing antigen binding proteins specific for PCSK9 with reduced viscosity. The method proceeds by replacing residues in high viscosity variable domain subfamilies with residues in correlating low viscosity subfamilies. The method further comprises substituting residues in the Fc domain with residues associated with low viscosity and adding charged residues to the C-terminus of the Fc domain. The present invention further concerns antigen binding proteins produced by this method.Type: GrantFiled: June 11, 2021Date of Patent: May 28, 2024Assignee: AMGEN INC.Inventors: Joon Hoi Huh, Riki Stevenson, Pavel Bondarenko, Andrew Nichols, Da Ren, Neeraj Jagdish Agrawal, Richard Smith
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Publication number: 20230349912Abstract: Provided herein are methods of processing a polypeptide or protein for analysis, e.g., peptide mapping analysis by mass spectrometry. In exemplary embodiments, the method comprises incubating a digested sample at a mildly acidic pH and/or in the presence of a chaotrope, wherein the digested sample is produced by digesting a polypeptide with a protease to produce a digested sample comprising at least two peptides. In exemplary embodiments, the method comprises digesting the polypeptide with a protease which cleaves C-terminal to tryptophan to produce a digested sample comprising at least two peptides. In exemplary embodiments, the method comprises digesting the polypeptide with trypsin at an enzyme:substrate (E:S) weight ratio of about 1:1 to about 1:15 to produce a digested sample comprising at least two peptides. In exemplary aspects, the digested sample comprises at least one or two peptides each comprising a tyrosine at the C-terminus.Type: ApplicationFiled: September 17, 2021Publication date: November 2, 2023Applicant: AMGEN INC.Inventors: Gang XIAO, Pavel BONDARENKO, Liuqing SHI, Thomas M. DILLON, Da REN, Margaret Speed RICCI, Weidong CUI, John Joseph HARRAHY, Jake PAWLOWSKI, Andrew DYKSTRA, Dylan RIGGS
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Publication number: 20230235024Abstract: The present invention relates to variant Fc-containing molecules, such as antibodies and Fc-fusion molecules, having glycosylation characteristics favorable to large-scale production of therapeutic molecules containing such variant Fc.Type: ApplicationFiled: July 14, 2022Publication date: July 27, 2023Applicant: Amgen Inc.Inventors: Zhimei DU, Pranhitha REDDY, Randal B. BASS, Feng HE, William C. FANSLOW, III, Yuling ZHANG, Da REN, Randall R. KETCHEM
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Publication number: 20220404370Abstract: The disclosed methods are directed to detecting polypeptide fragments (“clips”) of parental polypeptides. Parental polypeptides and clips are optionally denatured and then fractionated using a matrix. After a first elution (high molecular weight fraction), an additional elution step retrieves a low molecular weight fraction containing clips. If the clips are an appropriate size for the targeted detection method, such as mass spectrometry, then analysis of this fraction proceeds separately from the high molecular weight fraction, or the clips fraction is mixed with the proteolyzed high molecular weight fraction before analysis. However, if the clips are too large for the intended analytical method(s), then the clips are also proteolyzed. The digested high molecular weight and low molecular weight fractions can be analyzed separately or combined. Analysis of combined samples favors clip quantitation because the clips are analyzed together with the remaining counterpart of the parental polypeptide.Type: ApplicationFiled: June 7, 2022Publication date: December 22, 2022Applicant: AMGEN INC.Inventors: Da REN, Izydor APOSTOL, Alexander Julian VEACH
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Patent number: 11427627Abstract: The present invention relates to variant Fc-containing molecules, such as antibodies and Fc-fusion molecules, having glycosylation characteristics favorable to largescale production of therapeutic molecules containing such variant Fc. Described herein are compositions and methods to improve glycosylation maturation of and to minimize the culture process-dependent effects of Fc-containing molecules, e.g., Fc-fusion molecules and antibodies. Creating single and multiple amino acid substitutions within the Fc domain with the aim to improve high mannose processing and glycosylation maturation.Type: GrantFiled: September 5, 2014Date of Patent: August 30, 2022Assignee: Amgen Inc.Inventors: Zhimei Du, Pranhitha Reddy, Randal B. Bass, Feng He, William C. Fanslow, III, Yuling Zhang, Da Ren, Randal R. Ketchem
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Patent number: 11385238Abstract: The disclosed methods are directed to detecting polypeptide fragments (“clips”) of parental polypeptides. Parental polypeptides and clips are optionally denatured and then fractionated using a matrix. After a first elution (high molecular weight fraction), an additional elution step retrieves a low molecular weight fraction containing clips. If the clips are an appropriate size for the targeted detection method, such as mass spectrometry, then analysis of this fraction proceeds separately from the high molecular weight fraction, or the clips fraction is mixed with the proteolyzed high molecular weight fraction before analysis. However, if the clips are too large for the intended analytical method(s), then the clips are also proteolyzed. The digested high molecular weight and low molecular weight fractions can be analyzed separately or combined. Analysis of combined samples favors clip quantitation because the clips are analyzed together with the remaining counterpart of the parental polypeptide.Type: GrantFiled: June 29, 2018Date of Patent: July 12, 2022Inventors: Da Ren, Izydor Apostol, Alexander Julian Veach
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Publication number: 20210371544Abstract: The present invention concerns a method for preparing antigen binding proteins specific for PCSK9 with reduced viscosity. The method proceeds by replacing residues in high viscosity variable domain subfamilies with residues in correlating low viscosity subfamilies. The method further comprises substituting residues in the Fc domain with residues associated with low viscosity and adding charged residues to the C-terminus of the Fc domain. The present invention further concerns antigen binding proteins produced by this method.Type: ApplicationFiled: June 11, 2021Publication date: December 2, 2021Applicant: AMGEN INC.Inventors: JOON HOI HUH, RIKI STEVENSON, PAVEL BONDARENKO, ANDREW NICHOLS, DA REN, NEERAJ JAGDISH AGRAWAL, RICHARD SMITH
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Publication number: 20210255075Abstract: The disclosed methods are directed to preparing polypeptides for multi-attribute analysis. The polypeptides are optionally denatured, reduced, and/or alkylated before being subjected to a first digestion. Following the first digestion the large and small fragments resulting from the digestion are separated from each other. A second digestion is then performed on the larger of the fragments. All of the fragments from the two digestions are then analyzed chromatographically, electrophoretically, or spectrometrically, or a combination of these methods. The methods are especially useful for the preparation of therapeutic polypeptides for analysis, especially those that are not easily cleaved.Type: ApplicationFiled: March 13, 2019Publication date: August 19, 2021Inventors: Da REN, Melissa SATO
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Publication number: 20210223260Abstract: The invention generally relates to methods for determining whether a peptide includes aspartate or isoaspartate. In certain aspects, methods of the invention involve binding an aspartate/isoaspartate residue in a peptide with a label to produce a labeled peptide. The labeled peptide is then ionized. The ionizing process causes the label to undergo rearrangement in a gas phase at a higher rate if the label is bound to the aspartate residue as compared to if the label is bound to the isoaspartate residue. The methods of the invention then involve performing a mass spectrometry analysis to detect the rearrangement of the label, thereby determining whether the peptide includes aspartate or isoaspartate.Type: ApplicationFiled: January 26, 2021Publication date: July 22, 2021Inventors: Stephen T. Ayrton, Robert Graham Cooks, Tawnya Flick, Da Ren
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Patent number: 11059908Abstract: The present invention concerns a method for preparing antigen binding proteins with reduced viscosity. The method proceeds by replacing residues in high viscosity variable domain subfamilies with residues in correlating low viscosity subfamilies. The method further comprises substituting residues in the Fc domain with residues associated with low viscosity and adding charged residues to the C-terminus of the Fc domain. The present invention further concerns antigen binding proteins produced by this method.Type: GrantFiled: September 28, 2017Date of Patent: July 13, 2021Assignee: AMGEN INC.Inventors: Joon Hoi Huh, Riki Stevenson, Pavel Bondarenko, Andrew Nichols, Da Ren, Neeraj Jagdish Agrawal
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Patent number: 10908165Abstract: The invention generally relates to methods for determining whether a peptide includes aspartate or isoaspartate. In certain aspects, methods of the invention involve binding an aspartate/isoaspartate residue in a peptide with a label to produce a labeled peptide. The labeled peptide is then ionized. The ionizing process causes the label to undergo rearrangement in a gas phase at a higher rate if the label is bound to the aspartate residue as compared to if the label is bound to the isoaspartate residue. The methods of the invention then involve performing a mass spectrometry analysis to detect the rearrangement of the label, thereby determining whether the peptide includes aspartate or isoaspartate.Type: GrantFiled: June 7, 2018Date of Patent: February 2, 2021Assignees: Purdue Research Foundation, AMGEN INC.Inventors: Stephen T. Ayrton, Robert Graham Cooks, Tawnya Flick, Da Ren
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Patent number: 10520654Abstract: An ultra-thin broadband retardation film is provided. The broadband retardation film includes a first retardation film and a second retardation film. The second retardation film is disposed on a side of the first retardation film, wherein an in-plane retardation value Ro of the first retardation film is between 70 nm and 130 nm, an in-plane retardation value Ro of the second retardation film is between 140 nm and 260 nm, and an angle between an optic axis of the first retardation film and an optic axis of the second retardation film is between 35° and 70°.Type: GrantFiled: July 13, 2017Date of Patent: December 31, 2019Assignee: imat corporationInventors: Tsai-An Yu, Da-Ren Chiou, Shih-Ming Hung, Peng-Shun Tsai, Yi-Ting Chiu, Kuo-Chen Wu, Yi-Chien Chen
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Publication number: 20190378883Abstract: A display device is provided. The display device includes an organic light emitting diode display panel, a blue light brightness enhancement film and a polarizing film. The blue light brightness enhancement film is disposed on the organic light emitting diode display panel. The blue light brightness enhancement film includes a cholesteric liquid crystal layer and a quarter-wave phase retardation film, and the quarter-wave phase retardation film is disposed on the cholesteric liquid crystal layer. The polarizing film is disposed on the blue light brightness enhancement film.Type: ApplicationFiled: September 4, 2018Publication date: December 12, 2019Applicant: imat corporationInventors: Tsai-An Yu, Da-Ren Chiou, Shih-Ming Hung, Kuo-Chen Wu, Hui-Mei Liu, Hsiu-Yun Hsu
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Publication number: 20190233543Abstract: The present invention concerns a method for preparing antigen binding proteins with reduced viscosity. The method proceeds by replacing residues in high viscosity variable domain subfamilies with residues in correlating low viscosity subfamilies. The method further comprises substituting residues in the Fc domain with residues associated with low viscosity and adding charged residues to the C-terminus of the Fc domain. The present invention further concerns antigen binding proteins produced by this method.Type: ApplicationFiled: September 28, 2017Publication date: August 1, 2019Applicant: AMGEN INC.Inventors: Neeraj Jagdish AGRAWAL, Pavel BONDARENKO, Joon Hoi HUH, Andrew NICHOLS, Da REN, Richard SMITH, Riki STEVENSON
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Publication number: 20190204486Abstract: An optical film with a conductive function is provided. The optical film with the conductive function includes a liquid crystal optical compensation film and a conductive layer. The conductive layer is disposed on the liquid crystal optical compensation film.Type: ApplicationFiled: July 9, 2018Publication date: July 4, 2019Applicant: imat corporationInventors: Tsai-An Yu, Da-Ren Chiou, Shih-Ming Hung, Kuo-Chen Wu, Yi-Chien Chen, Hsiu-Yun Hsu
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Publication number: 20190004060Abstract: The disclosed methods are directed to detecting polypeptide fragments (“clips”) of parental polypeptides. Parental polypeptides and clips are optionally denatured and then fractionated using a matrix. After a first elution (high molecular weight fraction), an additional elution step retrieves a low molecular weight fraction containing clips. If the clips are an appropriate size for the targeted detection method, such as mass spectrometry, then analysis of this fraction proceeds separately from the high molecular weight fraction, or the clips fraction is mixed with the proteolyzed high molecular weight fraction before analysis. However, if the clips are too large for the intended analytical method(s), then the clips are also proteolyzed. The digested high molecular weight and low molecular weight fractions can be analyzed separately or combined. Analysis of combined samples favors clip quantitation because the clips are analyzed together with the remaining counterpart of the parental polypeptide.Type: ApplicationFiled: June 29, 2018Publication date: January 3, 2019Applicant: AMGEN INC.Inventors: Da REN, Izydor APOSTOL, Alexander Julian VEACH
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Publication number: 20180356426Abstract: The invention generally relates to methods for determining whether a peptide includes aspartate or isoaspartate. In certain aspects, methods of the invention involve binding an aspartate/isoaspartate residue in a peptide with a label to produce a labeled peptide. The labeled peptide is then ionized. The ionizing process causes the label to undergo rearrangement in a gas phase at a higher rate if the label is bound to the aspartate residue as compared to if the label is bound to the isoaspartate residue. The methods of the invention then involve performing a mass spectrometry analysis to detect the rearrangement of the label, thereby determining whether the peptide includes aspartate or isoaspartate.Type: ApplicationFiled: June 7, 2018Publication date: December 13, 2018Applicants: Purdue Research Foundation, AMGENInventors: Stephen T. Ayrton, Robert Graham Cooks, Tawnya Flick, Da Ren
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Publication number: 20180313989Abstract: A polarizing plate with optical compensation function is provided. The polarizing plate includes a polarizer, a liquid crystal optical compensation film, a first adhesive layer, an adhesion-promoting layer, a protective film, and a second adhesive layer. The liquid crystal optical compensation film is disposed on one side of the polarizer. The first adhesive layer is disposed between the polarizer and the liquid crystal optical compensation film. The adhesion-promoting layer is disposed between the first adhesive layer and the liquid crystal optical compensation film. The protective film is disposed on one side of the polarizer relative to the liquid crystal optical compensation film. The second adhesive layer is disposed between the polarizer and the protective film.Type: ApplicationFiled: January 11, 2018Publication date: November 1, 2018Applicant: Imat CorporationInventors: Tsai-An Yu, Da-Ren Chiou, Shih-Ming Hung, Kuo-Chen Wu, Yi-Chien Chen
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Publication number: 20180017723Abstract: An ultra-thin broadband retardation film is provided. The broadband retardation film includes a first retardation film and a second retardation film. The second retardation film is disposed on a side of the first retardation film, wherein an in-plane retardation value Ro of the first retardation film is between 70 nm and 130 nm, an in-plane retardation value Ro of the second retardation film is between 140 nm and 260 nm, and an angle between an optic axis of the first retardation film and an optic axis of the second retardation film is between 35° and 70°.Type: ApplicationFiled: July 13, 2017Publication date: January 18, 2018Applicant: imat corporationInventors: Tsai-An Yu, Da-Ren Chiou, Shih-Ming Hung, Peng-Shun Tsai, Yi-Ting Chiu, Kuo-Chen Wu, Yi-Chien Chen
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Patent number: D990545Type: GrantFiled: April 26, 2021Date of Patent: June 27, 2023Inventor: Da Ren Wang