Patents by Inventor Daguang Wang
Daguang Wang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8067581Abstract: Compounds relating to attachment chemistries for binding biomolecules to a substrate surface are described. These include compounds of the following structure: The biomolecule includes a single nucleic acid, oligonucleotides, polynucleotides, DNAs, RNAs, proteins, peptides, enzymes, antibodies, CNAs (cyclohexyl nucleic acids), p-MeNAs (methyl or methoxy phosphate nucleic acids), peptide nucleic acids (PNAs), and pyranosyl RNAs (p-RNAs).Type: GrantFiled: November 15, 2010Date of Patent: November 29, 2011Assignee: Sanofi-Aventis S.A.Inventors: Markus Schweitzer, Norbert Windhab, John R. Havens, Thomas J. Onofrey, Charles Greef, Daguang Wang
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Publication number: 20110201080Abstract: Compounds relating to attachment chemistries for binding biomolecules to a substrate surface are described. These include compounds of the following structure: The biomolecule includes a single nucleic acid, oligonucleotides, polynucleotides, DNAs, RNAs, proteins, peptides, enzymes, antibodies, CNAs (cyclohexyl nucleic acids), p-MeNAs (methyl or methoxy phosphate nucleic acids), peptide nucleic acids (PNAs), and pyranosyl RNAs (p-RNAs).Type: ApplicationFiled: November 15, 2010Publication date: August 18, 2011Inventors: Markus Schweitzer, Norbert Windhab, John R. Havens, Thomas J. Onofrey, Charles Greef, Daguang Wang
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Patent number: 7833715Abstract: Methods of binding biomolecules to a substrate are provided that include contacting the biomolecule with a branched linking moiety to form a branched linking structure. The branched linking structure is then contacted with a binding moiety on the substrate to form a coupled substrate binding structure, thereby binding the biomolecule to the substrate. The biomolecule may contain a Lewis base or a nucleophile to react with a Lewis acid or electrophile in the branched linking moiety. Alternatively, the biomolecule may contain a Lewis acid or electrophile that can react with a Lewis base or nucleophile in the branched linking moiety. Additionally, the biomolecule can be bound to the substrate through a covalent or non-covalent bond.Type: GrantFiled: January 18, 2007Date of Patent: November 16, 2010Assignee: Nanogen Recognomics GmbHInventors: Markus Schweitzer, Norbert Windhab, John R. Havens, Thomas J. Onofrey, Charles Greef, Daguang Wang
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Publication number: 20100286377Abstract: Methods of binding biomolecules to a substrate are provided that include contacting the biomolecule with a branched linking moiety to form a branched linking structure. The branched linking structure is then contacted with a binding moiety on the substrate to form a coupled substrate binding structure, thereby binding the biomolecule to the substrate. The biomolecule may contain a Lewis base or a nucleophile to react with a Lewis acid or electrophile in the branched linking moiety. Alternatively, the biomolecule may contain a Lewis acid or electrophile that can react with a Lewis base or nucleophile in the branched linking moiety. Additionally, the biomolecule can be bound to the substrate through a covalent or non-covalent bond.Type: ApplicationFiled: January 18, 2007Publication date: November 11, 2010Inventors: Markus Schweitzer, Norbert Windhab, John R. Havens, Thomas J. Onofrey, Charles Greef, Daguang Wang
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Patent number: 7723478Abstract: Disclosed are nucleic acid molecules encoding novel DKR polypeptides. Also disclosed are methods of preparing the nucleic acid molecules and polypeptides, and methods of using these molecules.Type: GrantFiled: August 14, 2007Date of Patent: May 25, 2010Assignee: Amgen Inc.Inventors: Michael Brian Bass, John Kevin Sullivan, Lars Eyde Theill, Daguang Wang
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Patent number: 7597932Abstract: The present invention provides improved synthetic polymer hydrogel permeation layers for use on active electronic matrix devices for biological assays. The present invention includes methods for forming a permeation layer on an array of microelectrodes including the steps of attaching a linker to the surface of the array and providing a polymerization solution that includes a porogen. The surface of the array is then contacted with the polymerization solution and the polymerization solution is then polymerized on the surface of the array to form a permeation layer that is attached o the surface of the array through the linker. The porogen is then removed from the permeation layer, thereby creating void spaces in the permeation layer.Type: GrantFiled: September 14, 2007Date of Patent: October 6, 2009Assignee: Nanogen, Inc.Inventors: Jainamma Krotz, Daniel Smolko, Howard R. Reese, Thomas J. Onofrey, Daguang Wang, Theodore M. Winger, John R. Havens
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Patent number: 7358057Abstract: The present invention relates e.g., to QM-7 or QT-6 cells comprising a heterologous mutant nicotinic ?7 acetylcholine receptor and/or a nucleic acid encoding it, or a fragment or variant thereof. In a preferred embodiment, the mutant nicotinic ?7 acetylcholine receptor subunit has a mutation in the M2 domain. QM-7 and QT-6 cells of the invention are useful for, e.g., assays such as high throughput assays that measure the influx of cations, such as Ca++ ions, into a cell. Such assays can be used, e.g., to identify agents that modulate the expression and/or activity of a mutant cell-surface-expressed channel receptor (e.g., the nicotinic ?7 receptor), and which thus modulate, e.g., among other functions, processes involved in the central nervous system, such as learning and memory.Type: GrantFiled: May 9, 2003Date of Patent: April 15, 2008Assignee: Memory Pharmaceuticals CorporationInventors: Daguang Wang, Michael De Vivo
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Publication number: 20080063794Abstract: The present invention provides improved synthetic polymer hydrogel permeation layers for use on active electronic matrix devices for biological assays. The present invention includes methods for forming a permeation layer on an array of microelectrodes including the steps of attaching a linker to the surface of the array and providing a polymerization solution that includes a porogen. The surface of the array is then contacted with the polymerization solution and the polymerization solution is then polymerized on the surface of the array to form a permeation layer that is attached o the surface of the array through the linker. The porogen is then removed from the permeation layer, thereby creating void spaces in the permeation layer.Type: ApplicationFiled: September 14, 2007Publication date: March 13, 2008Inventors: Jainamma Krotz, Daniel Smolko, Howard Reese, Thomas Onofrey, Daguang Wang, Theodore Winger, John Havens
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Publication number: 20080057540Abstract: Disclosed are nucleic acid molecules encoding novel DKR polypeptides. Also disclosed are methods of preparing the nucleic acid molecules and polypeptides, and methods of using these molecules.Type: ApplicationFiled: August 14, 2007Publication date: March 6, 2008Applicant: AMGEN INC.Inventors: Michael Bass, John Sullivan, Lars Theill, Daguang Wang
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Patent number: 7270850Abstract: The present invention provides improved synthetic polymer hydrogel permeation layers for use on active electronic matrix devices for biological assays. The present invention includes methods for forming a permeation layer on an array of microelectrodes including the steps of attaching a linker to the surface of the array by treating the surface with a linker by vapor deposition and providing a polymerization solution that includes at least one monomer having a polymerizable moiety, a modified streptavidin, a surfactant or porogen, and a cross-linking agent. The surface of the array is then contacted with the polymerization solution and the polymerization solution is then polymerized on the surface of the array to form a permeation layer that is attached o the surface of the array through the linker.Type: GrantFiled: March 16, 2005Date of Patent: September 18, 2007Assignee: Nanogen, Inc.Inventors: Jainamma Krotz, Daniel J. Smolko, Howard R. Reese, Thomas J. Onofrey, Daguang Wang, Theodore M. Winger, John R. Havens
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Patent number: 7186813Abstract: Biomolecules are provided having multiple binding sites for attachment to a substrate surface. The multiple attachment sites may be produced directly on the biomolecule or through use of branched phosphoramidite moieties that can be added in multiple to form dendritic structures which can in turn provide attachment sites for substrate binding moieties. Substrate binding moieties may include noncovalent binding moieties. For covalent binding moieties oligonucleotides containing hydrazides are provided. These hydrazides can be introduced via protected building blocks such as phosphoramidites or via building blocks containing precursor forms of such hydrazides.Type: GrantFiled: August 11, 2000Date of Patent: March 6, 2007Assignee: Nanogen Recognomics GmbHInventors: Markus Schweitzer, Norbert Windhab, John R. Havens, Thomas J. Onofrey, Charles Greef, Daguang Wang
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Publication number: 20060272038Abstract: The present invention provides for a recombinant nucleic acid molecule comprising a humanized mouse ?-amyloid precursor protein (“APP”) gene comprising K670N, M671L and V717F mutations and uses thereof. The present invention further provides for a recombinant nucleic acid molecule comprising a region of a calcium-calmodulin dependent kinase II? (“CaMKII?”) promoter operatively linked to a ?-amyloid precursor protein (“APP”) gene comprising at least one mutation and uses thereof.Type: ApplicationFiled: May 26, 2006Publication date: November 30, 2006Inventors: Michael De Vivo, Joerg Heyer, Mei Kwan, Daguang Wang, Qiurong Xiao
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Patent number: 7129229Abstract: This invention relates to attachment chemistries for binding macromolecules to a substrate surface or to other conjugation targets. More particularly, this invention relates to attachment chemistries involving branched or linear structures having one or more hydrazide attachment moieties for binding the macromolecules to a substrate surface, or for other conjugation reactions. Novel modifying reagents are provided for the introduction of protected hydrazide attachment moieties or precursor forms of such hydrazides to the macromolecule, either as a single hydrazide or as multiple hydrazides.Type: GrantFiled: August 10, 2001Date of Patent: October 31, 2006Assignee: Nanogen Recognomics GmbHInventors: Stefan Raddatz, Jochen Müller-Ibeler, Markus Schweitzer, Christoph Brücher, Norbert Windhab, John R. Havens, Thomas J. Onofrey, Charles H. Greef, Daguang Wang
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Publication number: 20060168668Abstract: The invention relates to phosphodiesterase PDE2A isoforms, as well as nucleic acids encoding such polypeptides. The invention further relates to methods for utilizing such polypeptides in diagnostic assays and in methods of screenin potential modulators, especially inhibitors, of the novel PDE2A disclosed herein. The polypeptides of the invention are involved in many physiological processes including, e.g. the formation of memory, cognitive function, and other disease and conditions which involve or are mediated by PDE2A and its signaling pathways.Type: ApplicationFiled: April 1, 2004Publication date: July 27, 2006Applicant: Memory Pharmaceuticals CorporationInventors: Daguang Wang, Bozena Bugaj-Gaweda
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Publication number: 20060166316Abstract: The invention relates to mammalian phosphodiesterase PDE10A7 isoforms, as well as the nucleic acids encoding such polypeptides. The invention further relates to methods for utilizing such polypeptides in diagnostic assays and in methods of screening potential modulators, especially inhibitors, of the novel PDE10A7 disclosed herein. The polypeptides of the invention are involved in many physiological processes including, e.g., the formation of memory, psychiatric disorders, and cognitive disorders.Type: ApplicationFiled: March 30, 2004Publication date: July 27, 2006Applicant: Memory Pharmaceuticals CorporationInventors: Daguang Wang, Bozena Bugaj-Gaweda
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Publication number: 20060142188Abstract: Monkey alpha-7 neuronal nicotinic acetylcholine receptor polypeptides, as well as the DNA (RNA) encoding such polypeptides, are disclosed. Also disclosed are methods for utilizing such polypeptides in diagnostic assays for identifying mutations in nucleic acid sequences encoding the polypeptides of the present invention, for detecting altered levels of the polypeptide of the present invention as a means of detecting diseases and methods of screening potential modulators of the novel alpha-7 receptor disclosed herein. Transgenic animals expressing polypeptides disclosed herein are also described.Type: ApplicationFiled: February 13, 2004Publication date: June 29, 2006Applicant: Memory Pharmaceuticals CorporationInventors: Shaojie Wang, Daguang Wang
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Publication number: 20050289660Abstract: Abstract of the Disclosure Human, rat and mouse cAMP phosphodiesterase isoforms (denoted PDE4D7s), as well as the DNA (RNA) encoding such polypeptides, are disclosed. Also disclosed are methods for utilizing such polypeptides in diagnostic assays for identifying mutations in nucleic acid sequences encoding the polypeptides of the present invention, for detecting altered levels of the polypeptide of the present invention as a means of detecting diseases and methods of screening potential modulators, especially inhibitors, of the novel PDE4D7s disclosed herein. Such as inhibitors can be used, for example, as a means of increasing cyclic AMP in neurons and thus treating neurological problems, such as long term memory loss, if not preventing such maladies entirely. Transgenic animals expressing polypeptides disclosed herein are also described.Type: ApplicationFiled: December 2, 2004Publication date: December 29, 2005Applicant: Memory Pharmaceuticals CorporationInventors: Daguang Wang, Xiaonan Xin, Axel Unterbeck, Christopher Leonard, Michael De Vivo
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Patent number: 6960298Abstract: The present invention provides improved synthetic polymer hydrogel permeation layers for use on active electronic matrix devices for biological assays. The permeation layers have a defined porous character, with mesopores in a size range between about 100 nanometers and about 1000 nanometers, and may also have micropores in the micrometer size range. The mesoporous synthetic hydrogel permeation layers demonstrate improved signal intensity and linearity characteristics as compared to nanoporous synthetic hydrogel permeation layers on active electronic matrix devices. In addition, the present invention also provides synthetic polymer hydrogel permeation layers which contain copolymerized attachment sites for nucleic acid probes or other biomolecules.Type: GrantFiled: December 10, 2001Date of Patent: November 1, 2005Assignee: Nanogen, Inc.Inventors: Jainamma Krotz, Daniel Smolko, Howard R. Reese, Thomas J. Onofrey, Daguang Wang, Theodore M. Winger, John R. Havens
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Publication number: 20050164283Abstract: The present invention provides improved synthetic polymer hydrogel permeation layers for use on active electronic matrix devices for biological assays. The present invention includes methods for forming a permeation layer on an array of microelectrodes including the steps of attaching a linker to the surface of the array by treating the surface with a linker by vapor deposition and providing a polymerization solution that includes at least one monomer having a polymerizable moiety, a modified streptavidin, a surfactant or porogen, and a cross-linking agent. The surface of the array is then contacted with the polymerization solution and the polymerization solution is then polymerized on the surface of the array to form a permeation layer that is attached o the surface of the array through the linker.Type: ApplicationFiled: March 16, 2005Publication date: July 28, 2005Inventors: Jainamma Krotz, Daniel Smolko, Howard Reese, Thomas Onofrey, Daguang Wang, Theodore Winger, John Havens
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Patent number: D523717Type: GrantFiled: September 16, 2004Date of Patent: June 27, 2006Assignee: Qingdao D&D Electro Mechanical Technologies Co., Ltd.Inventors: Jianjin Guo, Daguang Wang