Patents by Inventor Daizo Tokinaga
Daizo Tokinaga has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8142635Abstract: A capillary array includes a light detection portion, a sample supply portion, a buffer solution supply portion and a voltage application portion which are necessary functions for electrophoresis, thereby, when assembling the capillary array into an electrophoresis apparatus, the same can be immediately used. Accordingly, a capillary array is provided which can be easily incorporated into an electrophoresis apparatus.Type: GrantFiled: July 26, 2010Date of Patent: March 27, 2012Assignee: Hitachi, Ltd.Inventors: Yasushi Shimizu, Toshiaki Kita, Kiyoshi Tsukada, Akihiro Suzuki, Tomonari Morioka, Masaya Kojima, Ryoji Inaba, Daizo Tokinaga, Shuhei Yamamoto, Yoshiyuki Okishima, Masao Fukunaga, Takayasu Furukawa, Tomohiro Shoji
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Publication number: 20100288642Abstract: A capillary array includes a light detection portion, a sample supply portion, a buffer solution supply portion and a voltage application portion which are necessary functions for electrophoresis, thereby, when assembling the capillary array into an electrophoresis apparatus, the same can be immediately used. Accordingly, a capillary array is provided which can be easily incorporated into an electrophoresis apparatus.Type: ApplicationFiled: July 26, 2010Publication date: November 18, 2010Inventors: Yasushi Shimizu, Toshiaki Kita, Kiyoshi Tsukada, Akihiro Suzuki, Tomonari Morioka, Masaya Kojima, Ryoji Inaba, Daizo Tokinaga, Shuhei Yamamoto, Yoshiyuki Okishima, Masao Fukunaga, Takayasu Furukawa, Tomohiro Shoji
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Patent number: 7811451Abstract: The flow rate of a solvent is reduced as a separation column and the separation performance is improved even under high-pressure conditions. There is provided a separation column including a monolith rod into which a sample and a mobile phase flow, the separation column comprising: a coating material coated on the outer circumference of a monolith rod; a support member into which the monolith rod coated with the coating material is inserted, and a rod fixing material fitted into or filled a gap between the coating material and the support member; wherein the upper end face of the rod fixing material is sealed, the upper end face being the inflow-side end when the separation column is assembled.Type: GrantFiled: October 24, 2007Date of Patent: October 12, 2010Assignee: Hitachi High-Technologies CorporationInventors: Yoshihiro Nagaoka, Masahito Ito, Daizo Tokinaga, Tomohiro Shoji, Tomonari Morioka
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Patent number: 7785458Abstract: A capillary array includes a light detection portion, a sample supply portion, a buffer solution supply portion and a voltage application portion which are necessary functions for electrophoresis, thereby, when assembling the capillary array into an electrophoresis apparatus, the same can be immediately used. Accordingly, a capillary array is provided which can be easily incorporated into an electrophoresis apparatus.Type: GrantFiled: December 15, 2005Date of Patent: August 31, 2010Assignee: Hitachi, Ltd.Inventors: Yasushi Shimizu, Toshiaki Kita, Kiyoshi Tsukada, Akihiro Suzuki, Tomonari Morioka, Masaya Kojima, Ryoji Inaba, Daizo Tokinaga, Shuhei Yamamoto, Yoshiyuki Okishima, Masao Fukunaga, Takayasu Furukawa, Tomohiro Shoji
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Publication number: 20100012566Abstract: The flow rate of a solvent is reduced as a separation column and the separation performance is improved even under high-pressure conditions. There is provided a separation column including a monolith rod into which a sample and a mobile phase flow, the separation column comprising: a coating material coated on the outer circumference of a monolith rod; a support member into which the monolith rod coated with the coating material is inserted, and a rod fixing material fitted into or filled a gap between the coating material and the support member; wherein the upper end face of the rod fixing material is sealed, the upper end face being the inflow-side end when the separation column is assembled.Type: ApplicationFiled: September 24, 2009Publication date: January 21, 2010Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATIONInventors: Yoshihiro NAGAOKA, Masahito ITO, Daizo TOKINAGA, Tomohiro SHOJI, Tomonari MORIOKA
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Publication number: 20080245716Abstract: A monolithic separation column and a liquid chromatography system using the same are provided by which the pressure tightness is improved, and the manufacturing is enabled at a low temperature, thus preventing the degradation of the separation performance. A monolithic rod 118 is made of a porous body and has a cylindrical shape. A coat material 116 coats an outer circumference of the monolithic rod 118 and includes an uneven portion 116R on an outer face thereof A holder 112, into which the monolithic rod 118 coated with the coat material 116 is inserted, includes a taper portion 112T on an inner face thereof. A potting media 114 is fitted or filled between the coat material 116 and the holder 112.Type: ApplicationFiled: March 21, 2008Publication date: October 9, 2008Inventors: Takeshi Ohura, Tomohiro Shoji, Daizo Tokinaga, Tomonari Morioka, Yoshihiro Nagaoka, Yoshitaka Kodama
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Publication number: 20080099389Abstract: The flow rate of a solvent is reduced as a separation column and the separation performance is improved even under high-pressure conditions. There is provided a separation column including a monolith rod into which a sample and a mobile phase flow, the separation column comprising: a coating material coated on the outer circumference of a monolith rod; a support member into which the monolith rod coated with the coating material is inserted, and a rod fixing material fitted into or filled a gap between the coating material and the support member; wherein the upper end face of the rod fixing material is sealed, the upper end face being the inflow-side end when the separation column is assembled.Type: ApplicationFiled: October 24, 2007Publication date: May 1, 2008Inventors: Yoshihiro Nagaoka, Masahito Ito, Daizo Tokinaga, Tomohiro Shoji, Tomonari Morioka
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Patent number: 7316770Abstract: A method of electrophoresis, an electrophoresis apparatus and a capillary array in which a fluorescently labelled sample is supplied into capillaries in a capillary array constituted by a plurality of capillaries and including a sample supply portion, an electrophoresis medium supply portion and a detection portion while controlling the temperature of the capillary array through gas circulation, the sample is caused to migrate and separated in the capillaries through electrophoresis, and at the region where the capillaries are contacted or come close each other a plurality of capillaries are contacted to a solid body so as to dissipate heat generated from the capillaries to the solid body.Type: GrantFiled: March 23, 2001Date of Patent: January 8, 2008Assignee: Hitachi, Ltd.Inventors: Ryoji Inaba, Tomoyuki Sakai, Satoshi Takahashi, Muneo Maeshima, Miho Ozawa, Masaya Kojima, Daizo Tokinaga
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Publication number: 20060091012Abstract: A capillary array includes a light detection portion, a sample supply portion, a buffer solution supply portion and a voltage application portion which are necessary functions for electrophoresis, thereby, when assembling the capillary array into an electrophoresis apparatus, the same can be immediately used. Accordingly, a capillary array is provided which can be easily incorporated into an electrophoresis apparatus.Type: ApplicationFiled: December 15, 2005Publication date: May 4, 2006Inventors: Yasushi Shimizu, Toshiaki Kita, Kiyoshi Tsukada, Akihiro Suzuki, Tomonari Morioka, Masaya Kojima, Ryoji Inaba, Daizo Tokinaga, Shuhei Yamamoto, Yoshiyuki Okishima, Masao Fukunaga, Takayasu Furukawa, Tomohiro Shoji
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Publication number: 20060076239Abstract: In an electrophoresis apparatus comprising capillaries for containing therein fluorescence-labeled substances, and a sensor for irradiating the capillaries with exciting light and detecting fluorescences, the sensor has a substrate including a datum surface for holding thereon the capillaries, and a groove sinking from the datum surface, and the groove has a plurality of reflecting surfaces for reflecting thereon the fluorescences emitted from the capillaries by a plurality of times.Type: ApplicationFiled: October 7, 2005Publication date: April 13, 2006Inventors: Ryoji Inaba, Motohiro Yamazaki, Akihiro Suzuki, Miho Ozawa, Daizo Tokinaga, Tomonari Morioka, Seiichi Ugai
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Patent number: 7005052Abstract: A capillary array includes a light detection portion, a sample supply portion, a buffer solution supply portion and a voltage application portion which are necessary functions for electrophoresis, thereby, when assembling the capillary array into an electrophoresis apparatus, the same can be immediately used. Accordingly, a capillary array is provided which can be easily incorporated into an electrophoresis apparatus.Type: GrantFiled: May 1, 2001Date of Patent: February 28, 2006Assignee: Hitachi, Ltd.Inventors: Yasushi Shimizu, Toshiaki Kita, Kiyoshi Tsukada, Akihiro Suzuki, Tomonari Morioka, Masaya Kojima, Ryoji Inaba, Daizo Tokinaga, Shuhei Yamamoto, Yoshiyuki Okishima, Masao Fukunaga, Takayasu Furukawa, Tomohiro Shoji
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Publication number: 20050067285Abstract: The present invention is related to the decrease of crosstalk, in which part of light emission from a specific capillary is overlayed on the light emission position of its adjoining capillaries and is detected as signal from adjacent capillaries. Study conducted by the inventor has found that the crosstalk contains at least the following components. The signal light from a capillary may propagate through an adjoining capillary or a plurality of capillaries, the reflection at the inner surface of the outer diameter of quartz capillary makes plural signal paths to the photodetector. The crosstalk component may be focused at a point at a predetermined distance from the center axis of the capillary. The present invention is aimed at the control of the range of image to be detected as the capillary signal among the images focused on the photodetector from the capillaries. The present invention may decrease the crosstalk by controlling the detection of the light emission from the adjacent capillaries.Type: ApplicationFiled: August 9, 2004Publication date: March 31, 2005Inventors: Ryoji Inaba, Miho Ozawa, Akihiro Suzuki, Daizo Tokinaga, Tomohiro Shoji, Yoshitaka Kodama, Isao Haraura, Masaya Kojima
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Publication number: 20020023839Abstract: A method of electrophoresis, an electrophoresis apparatus and a capillary array in which a fluorescently labelled sample is supplied into capillaries in a capillary array constituted by a plurality of capillaries and including a sample supply portion, an electrophoresis medium supply portion and a detection portion while controlling the temperature of the capillary array through gas circulation, the sample is caused to migrate and separated in the capillaries through electrophoresis, and at the region where the capillaries are contacted or come close each other a plurality of capillaries are contacted to a solid body so as to dissipate heat generated from the capillaries to the solid body.Type: ApplicationFiled: March 23, 2001Publication date: February 28, 2002Inventors: Ryoji Inaba, Tomoyuki Sakai, Satoshi Takahashi, Muneo Maeshima, Miho Ozawa, Masaya Kojima, Daizo Tokinaga
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Publication number: 20010040095Abstract: A capillary array includes a light detection portion, a sample supply portion, a buffer solution supply portion and a voltage application portion which are necessary functions for electrophoresis, thereby, when assembling the capillary array into an electrophoresis apparatus, the same can be immediately used. Accordingly, a capillary array is provided which can be easily incorporated into an electrophoresis apparatus.Type: ApplicationFiled: May 1, 2001Publication date: November 15, 2001Inventors: Yasushi Shimizu, Toshiaki Kita, Kiyoshi Tsukada, Akihiro Suzuki, Tomonari Morioka, Masaya Kojima, Ryoji Inaba, Daizo Tokinaga, Shuhei Yamamoto, Yoshiyuki Okishima, Masao Fukunaga, Takayasu Furukawa, Tomohiro Shoji
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Patent number: 5308990Abstract: A microparticle measuring method according to the present invention, by which the number of fluorescent microparticles is counted and the fluorescent microparticles are analyzed, includes the steps of introducing fluorescent microparticles into a narrow flow path almost one after another; irradiating the fluorescent microparticles in the narrow flow path with excitation light; detecting a signal pulse produced by detection of a single photon of fluorescence generated by the irradiation with the excitation light; and recognizing existence of the fluorescent microparticle, starting from the number of signal pulses measured per predetermined standard period, and further includes the step of obtaining the number of signal pulses per standard period with a time interval shorter than the standard period.Type: GrantFiled: May 14, 1992Date of Patent: May 3, 1994Assignee: Hitachi, Ltd.Inventors: Satoshi Takahashi, Daizo Tokinaga, Kazunori Okano
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Patent number: 5228973Abstract: A body fluid sample is diluted with a diluent which comprises an aqueous solution containing bis(2-hydroxyethyl)-iminotris(hydroxymethyl)methane and boric acid to obtain a diluted body fluid sample, and the ion concentration in the diluted body fluid sample is determined with ion-selective electrodes, whereby the variation width of pH in the diluted body fluid sample can be held down to a narrow range. As a result the determination can be made without exerting adverse effects on the ion-selective electrodes, and errors in determination can be markedly reduced as compared with cases where the conventional diluents are used.Type: GrantFiled: July 30, 1991Date of Patent: July 20, 1993Assignees: Hitachi, Ltd., Mitsuo SekiguchiInventors: Mitsuo Sekiguchi, Yoshiteru Furuta, Daizo Tokinaga
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Patent number: 5057438Abstract: Determination of a plurality of species of antibodies or antigens is attained by a method which comprises forming a plurality of different kinds of reaction membranes each having a different species of antibody or antigen on an electrophoretic carrier, superposing these reaction membranes, optionally superposing a filter on the laminate of reaction membranes, inserting the laminate in an electrolyte, adding a plurality of different species of antigens or antibodies corresponding to the plurality of species of antibodies or antigens supported in the aforementioned reaction membranes, electrophoretically moving the added antigens or antibodies through the electrolyte and enabling them to react with the antibodies or antigens supported on the reaction membranes, and measuring the concentrations of either the antigens or antibodies resulting from the reaction or the antibodies or antigens supported in an unreacted form on the reaction membranes.Type: GrantFiled: March 30, 1987Date of Patent: October 15, 1991Assignees: Agency of Industrial Science and Technology, Ministry of International Trade and IndustryInventors: Kazumichi Imai, Daizo Tokinaga, Teruaki Kobayashi, Kenji Yasuda, Keiichi Nagai, Satoshi Takahashi
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Patent number: 4913883Abstract: The degree of agglutination is measured in a magnetic manner instead of optical manner. Microparticles are composed of a magnetic material and the agglutinated condition is measured using a magnetometer. The sizes and distribution of agglutinated materials formed by the antigen-antibody coupling reaction can be correctly measured without affected by light-scattering materials such as proteins or blood cells contained in the specimen, or by absorbant such as pigment, or by fluorescent material. Therefore, the concentration of antigen is measured maintaining high precision.Type: GrantFiled: July 20, 1988Date of Patent: April 3, 1990Assignee: Hitachi, Ltd.Inventors: Kazumichi Imai, Daizo Tokinaga, Koichi Yokosawa
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Patent number: 4824778Abstract: An immunoassay comprisingimmobilizing antibody in a matrix for electrophoresis;immobilizing antigen in a measurement sample by subjecting the same to antigen antibody reaction with the above-mentioned immobilized antibody by a procedure of moving the antigen by electrophoresis;either moving labeled antibody to the above-mentioned immobilized antigen by electrophoresis to react the same with the immobilized antigen, or moving labeled antigen to the unreacted portion of the above-mentioned immobilized antibody by electrophoresis to react the same with the unreacted portion; andmeasuring the concentration of antigen in the sample, characterized byusing as a label for the labeled antibody or the labeled antigen an enzyme capable of coverting a substrate into a fluorescent substance,moving the substrate convertible into a fluorescent substance by said enzyme by electrophoresis,reacting the substrate with the label enzyme to convert the same into a fluorescent substance, andmeasuring the concentration of the fluoreType: GrantFiled: May 28, 1986Date of Patent: April 25, 1989Assignee: Hitachi, Ltd.Inventors: Keiichi Nagai, Daizo Tokinaga, Kazumichi Imai, Kenji Yasuda, Satoshi Takahashi, Teruaki Kobayashi
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Patent number: 4628035Abstract: An immunoassay method for measuring a concentration of an antigen for a short period of time by immobilizing an antibody over the whole zone of an effective supporting matrix for electrophoresis and fixing an antigen in a sample to be measured by electrophoresis for the antigen-antibody reaction between said immobilized antibody and said antigen.Type: GrantFiled: August 7, 1984Date of Patent: December 9, 1986Assignee: Hitachi, Ltd.Inventors: Daizo Tokinaga, Teruaki Kobayashi, Kazumichi Imai