Abstract: We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Abstract: Disclosed herein are integrated microfluidic devices and methods of using the devices for large panel detection and multi-step procedures within a single, enclosed structure. The methods and devices provided herein are capable of two-dimensional and three-dimensional fluid pumping along a disc surface and among multiple discs. In an embodiment, one or more pumps are used to propel sample in a direction opposite the direction of centrifugal force such that sample flows both radially outward and radially inward relative to the disc's axis of rotation. This effectively provides an increase in usable disc space for the flow of sample. The disclosed devices and methods reduce, minimize, or eliminate the surface area limitation of known integrated microfluidic devices. Thus, the disclosed devices provide increased usable surface area of a rotating disc structure.

Abstract: We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Abstract: We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Abstract: Microreaction molds and methods of molding very thin films onto substrate surfaces are provided. The molds and molding methods allow for consistency and uniformity in the thicknesses of the films that are applied to the substrate surfaces. The molds may be single composite, such as etched silica, or multicomposite, such as quartz/metal. The molds may further comprise an adjustable molding cavity. The molds of this invention are particularly applicable to generating thin polymeric films onto microchip substrates.

Abstract: A beverage container is provided with a hydrophobic vent consisting of a relatively thick and rigid disc-shaped piece of macroporous plastic having pore sizes averaging from 7-350 microns. The vent can be welded, molded or secured to the sidewall, bottom or cap of a plastic beverage container thus eliminating all moving parts. The macroporous plastic is resistant to oxidative abrasion, contamination and wetting and is strong enough to resist breakage. In one embodiment a baby bottle is provided which consists of a plastic bottle body, a nipple, and means for fastening the nipple to the bottle body. The bottle body is provided with a macroporous plastic vent which can be welded, molded or secured to the sidewall or bottom of the bottle body thus eliminating all moving parts. The bottle body can be washed repeatedly as a single unit with the vent intact.

Abstract: We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Abstract: We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.