Abstract: The present invention relates to a method of evaluating the cancer state of a subject using lecithin:retinol acyl transferase (LRAT) gene promoter methylation status. Methods of analyzing and quantifying LRAT gene promoter methylation level are also disclosed. The present invention also relates to methods of determining the prognosis for s subject having cancer by assessing LRAT mRNA expression and LRAT protein expression. Methods of cancer detection, diagnosis, prognosis, and treatment are also disclosed.

Abstract: Closures for containers and methods for using same are provided. In a general embodiment/the present disclosure provides a closure having a top portion (12), a bottom portion (14) and a side portion (16), an aperture (18) extending though the closure, a projection (20) extending from the closure and at least two rib members (36) on an interior of the projection. The projection may also include a cover (22). In another embodiment, a method for using a closure includes inserting a. spike member into a projection, piercing a membrane that hermetically seals a medical container, pushing rib members within the projection to center the spike member inserted into the projection, and tearing the membrane to create a vent hole in the membrane.

Abstract: The present invention relates to a method of evaluating the cancer state of a subject using lecithin:retinol acyl transferase (LRAT) gene promoter methylation status. Methods of analyzing and quantifying LRAT gene promoter methylation level are also disclosed. The present invention also relates to methods of determining the prognosis for s subject having cancer by assessing LRAT mRNA expression and LRAT protein expression. Methods of cancer detection, diagnosis, prognosis, and treatment are also disclosed.

Abstract: The present invention (also identified as “Capacitance cytometry”) relates to microfluidic and nanofluidic devices for detecting or measuring an electrical property of a fluid (liquid or aerosol), a single molecule, particle, or cell in fluid. In a particular embodiment, the devices detect or measure changes in capacitance of a fluid, molecule, particle or cell as it passes through the device. The invention relates to detection and measurement of single molecules, particularly biological molecules, and to methods of sequencing polynucleotide molecules (RNA or DNA) by detecting differentially labeled single nucleotides. Single molecule detection applications include DNA or RNA sequencing, detection of SNPs, protoemics, and particle sizing. The device can be used to determine cell DNA content, to analyze cell-cycle kinetics of cell populations, and to assay for abnormal changes in cell DNA content.

Abstract: Assays for determining the downstream effects of drugs that modulate the function of transcription regulatory proteins are disclosed. The assays comprise the steps of (a) providing cells that contain the transcription factor; (b) maintaining a control population and a test population of the cells under conditions that allow gene expression to occur in the cells, wherein the test population is exposed to the agent that modulates the activity of the transcription factor; (c) generating a gene expression profile for each of the control population and the test population of cells; and (d) comparing the gene expression profile from the control population of cells with the gene expression profile from the test population of cells; those differences being attributable to the effect of the agent that modulates the activity of the transcription factor.

Abstract: A coplanar waveguide for use in dielectric spectroscopy of biological solution is described. The waveguide's inner conductor can have a small gap and a sample containing space is laid over the gap. The sample containing space holds a small volume, ranging from a few picoliters to a few microliters of a biological solution. The waveguide is then driven with electrical signals across an extremely wide frequency range from 40 Hz to 40 GHz. The waveguide is coupled to a network or impedance analyzer by means of appropriate connectors and the response of the biological solution to the input signals is recorded. One-port and two-port measurements can be made without any modifications. The simple geometry of the waveguide makes it easy to integrate with microfluidic systems.

Abstract: A coplanar waveguide for use in dielectric spectroscopy of biological solution is described. The waveguide's inner conductor can have a small gap and a sample containing space is laid over the gap. The sample containing space holds a small volume, ranging from a few picoliters to a few microliters of a biological solution. The waveguide is then driven with electrical signals across an extremely wide frequency range from 40 Hz to 40 GHz. The waveguide is coupled to a network or impedance analyzer by means of appropriate connectors and the response of the biological solution to the input signals is recorded. One-port and two-port measurements can be made without any modifications. The simple geometry of the waveguide makes it easy to integrate with microfluidic systems.