Abstract: The invention includes an in vitro bleeding time device having an opening through a sheet of material across an outlet. At least a portion the sheet of material contains a coating which includes collagen type I, fibrinogen, fibronectin, and von Willebrand factor. The invention includes a blood coagulation analysis system which has a device with an internal chamber for receiving a blood sample through an inlet. A sheet of material spans a flowpath through a single device outlet. A controller regulates pressure and or flow rate within the device. The invention includes a method for analyzing blood coagulation. Blood provided into a device chamber flows out through an opening through a sheet of material which spans the device outlet. A controller is utilized to control pressure and/or flow rate within the device by controlling the flow rate into the device during formation of a clot blockage of the opening.

Abstract: The invention includes an in vitro bleeding time device having an opening through a sheet of material across an outlet. At least a portion the sheet of material contains a coating which includes collagen type I, fibrinogen, fibronectin, and von Willebrand factor. The invention includes a blood coagulation analysis system which has a device with an internal chamber for receiving a blood sample through an inlet. A sheet of material spans a flowpath through a single device outlet. A controller regulates pressure and or flow rate within the device. The invention includes a method for analyzing blood coagulation. Blood provided into a device chamber flows out through an opening through a sheet of material which spans the device outlet. A controller is utilized to control pressure and/or flow rate within the device by controlling the flow rate into the device during formation of a clot blockage of the opening.

Abstract: A clot lysis time determining device for determining the time necessary for fluid to lyse a clot. The device comprises: a body defining a chamber; a fluid receiving inlet port to the body in fluid communication with the chamber, the inlet port permitting fluid to flow into the chamber; a fluid discharging outlet port to the body in fluid communication with the chamber, the outlet port permitting fluid to flow out from the chamber; the inlet port, chamber, and outlet port defining a communicating fluid passageway; a body opening formed in the body along the fluid passageway, the body opening permitting a portion of fluid to flow against a clot supported by clot-supporting porous membrane; and a membrane cover detachably connectable to the body and configured for holding a clot-supporting porous membrane over the body opening.

Abstract: A clot lysis time determining device for determining the time necessary for fluid to lyse a clot. The device comprises: a body defining a chamber; a fluid receiving inlet port to the body in fluid communication with the chamber, the inlet port permitting fluid to flow into the chamber; a fluid discharging outlet port to the body in fluid communication with the chamber, the outlet port permitting fluid to flow out from the chamber; the inlet port, chamber, and outlet port defining a communicating fluid passageway; a body opening formed in the body along the fluid passageway, the body opening permitting a portion of fluid to flow against a clot supported by clot-supporting porous membrane; and a membrane cover detachably connectable to the body and configured for holding a clot-supporting porous membrane over the body opening.

Abstract: A low cost, disposable device for in vitro determination of bleeding time is detachably connectable to the end of a syringe. The device includes a hollow body having inlet and outlet ports and a detachable cover designed to hold a tissue-simulating membrane against the body. Openings are formed in the body and cover to expose the membrane to blood that is passed through the hollow body under pressure from the syringe. Blood flows through a slit in the membrane until it clots. Bleeding time is determined as the time elapsed before the blood stops flowing through the slit. A blood collection reservoir is provided about the body to collect blood discharged from the device to reduce health risks associated with handling blood.

Abstract: A method and kit for typing platelets in whole blood for human leucocyte antigens (HLA). A panel of HLA antigens is made on a microtiter plate. Each well is contacted with serum to be typed and the amount of reaction is followed with anti-human (Fab '2) alkaline phosphatase and disodium p-phenylphosphate.

Abstract: An apparatus and method for in vitro bleeding time determination. Blood is placed in a tube having a window covered with a clot promoting material having a slit. The time required before blood stops flowing through the slit is recorded, and is the bleeding time. It is preferred that the clot-promoting material be nylon fabric impregnated with collagen and blood clot promoting proteins.