Patents by Inventor Daniel F. Carlson
Daniel F. Carlson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240057570Abstract: Methods, uses, and animals for introgression of alleles between animals, including SNPs. One embodiment involves introducing a targeted targeting endonuclease system and a HDR template into a cell with a mismatch in the binding of the targeting endonuclease and the targeted site.Type: ApplicationFiled: September 19, 2022Publication date: February 22, 2024Inventors: Scott C. Fahrenkrug, Daniel F. Carlson
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Patent number: 11477969Abstract: Methods, uses, and animals for introgression of alleles between animals, including SNPs. One embodiment involves introducing a targeted targeting endonuclease system and a HDR template into a cell with a mismatch in the binding of the targeting endonuclease and the targeted site.Type: GrantFiled: September 20, 2018Date of Patent: October 25, 2022Assignee: Recombinetics, Inc.Inventors: Scott C. Fahrenkrug, Daniel F. Carlson
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Publication number: 20220056482Abstract: The present disclosure relates to methods for making genetic edits in vitro in a non-human vertebrate cell or embryo at a plurality of target chromosomal DNA sites. Methods for making a non-human animal having multiplex genetic edits at a plurality of target chromosomal DNA sites and making a non-human vertebrate animal chimeric for host cells and donor cells are also considered.Type: ApplicationFiled: July 19, 2021Publication date: February 24, 2022Inventors: Scott C. Fahrenkrug, Daniel F. Carlson
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Publication number: 20220015341Abstract: The present disclosure provides, at least, sterile fish and methods for producing sterile fish.Type: ApplicationFiled: October 7, 2021Publication date: January 20, 2022Inventors: Karl J. Clark, Tad S. Sonstegard, Daniel F. Carlson
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Publication number: 20210251200Abstract: The present disclosure provides methods to produce non-human animal models for diseases that have a poor life-expectancy. The animal models provided herein are the result of gene editing to result in genetic lesions that recapitulate human diseases by virtue of introgressing lethal, dominant negative or non-functional mutations in animal genomes corresponding to those responsible for human diseases. In some cases, the genomic edit may result in a low number of pregnancies carried to term and/or a failure to thrive phenotype with those born failing to survive to sexual maturity. The present disclosure provides methods to produce non-chimeric animals containing a detrimental genetic lesion from healthy chimeric animals. In this method, the chimeric animals are derived from cells in which the genetic lesion is made with the defect being complemented by the genome of an animal that is gametogenically deficient (cannot produce gametes) and cannot pass on its own genes.Type: ApplicationFiled: February 26, 2021Publication date: August 19, 2021Inventors: Daniel F. Carlson, Staci Lyn Solin
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Publication number: 20210185990Abstract: Methods, uses, and compositions for manipulating genomic DNA. Some of the embodiments of the invention provide for making a founder animal that is completely free of all unplanned genetic modifications. Some embodiments are directed to removing genetic faults in established breeds without making other alterations to the genome. Other embodiments are directed to particular tools or processes such as TALENs or CRISPR with a preferred truncation. One embodiment involves introducing a targeted targeting endonuclease system and a HDR template into a cell (optionally with a mismatch in the binding of the targeting endonuclease and the targeted site). Another embodiment includes processes of making a genetically modified livestock animal comprising a genome that comprises inactivation of a neuroendocrine gene selective for sexual maturation, with the inactivation of the gene preventing the animal from becoming sexually mature.Type: ApplicationFiled: December 10, 2020Publication date: June 24, 2021Inventors: Scott C. Fahrenkrug, Daniel F. Carlson
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Patent number: 10959414Abstract: Methods, uses, and animals for introgression of alleles between animals, including SNPs. One embodiment involves introducing a targeted targeting endonuclease system and a HDR template into a cell with a mismatch in the binding of the targeting endonuclease and the targeted site.Type: GrantFiled: November 2, 2017Date of Patent: March 30, 2021Assignee: Recombinetics, Inc.Inventors: Scott C. Fahrenkrug, Daniel F. Carlson
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Patent number: 10959415Abstract: Methods, uses, and compositions for manipulating genomic DNA. Some of the embodiments of the invention provide for making a founder animal that is completely free of all unplanned genetic modifications. Some embodiments are directed to removing genetic faults in established breeds without making other alterations to the genome. Other embodiments are directed to particular tools or processes such as TALENs or CRISPR with a preferred truncation. One embodiment involves introducing a targeted targeting endonuclease system and a HDR template into a cell (optionally with a mismatch in the binding of the targeting endonuclease and the targeted site). Another embodiment includes processes of making a genetically modified livestock animal comprising a genome that comprises inactivation of a neuroendocrine gene selective for sexual maturation, with the inactivation of the gene preventing the animal from becoming sexually mature.Type: GrantFiled: September 11, 2019Date of Patent: March 30, 2021Assignee: Recombinetics, Inc.Inventors: Scott C. Fahrenkrug, Daniel F. Carlson
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Patent number: 10920242Abstract: Methods, uses, and compositions for manipulating genomic DNA. Some of the embodiments of the invention provide for making a founder animal that is completely free of all unplanned genetic modifications. Some embodiments are directed to removing genetic faults in established breeds without making other alterations to the genome. Other embodiments are directed to particular tools or processes such as TALENs or CRISPR with a preferred truncation. One embodiment involves introducing a targeted targeting endonuclease system and a HDR template into a cell (optionally with a mismatch in the binding of the targeting endonuclease and the targeted site). Another embodiment includes processes of making a genetically modified livestock animal comprising a genome that comprises inactivation of a neuroendocrine gene selective for sexual maturation, with the inactivation of the gene preventing the animal from becoming sexually mature.Type: GrantFiled: May 28, 2019Date of Patent: February 16, 2021Assignee: Recombinetics, Inc.Inventors: Scott C. Fahrenkrug, Daniel F. Carlson
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Publication number: 20210037797Abstract: Disclosed herein, are inducible immunodeficient animals and methods to make them by adding an IL2Rg/RAG2 rescue cassette (RG-reg) or an IL2Rg/RAG2/FAH rescue cassette (FRG-reg) to a line of IL2Rg/RAG2 knockout (RG-KO) or IL2Rg/RAG2/FAH knockout (FRG-KO) swine. The rescue cassette enables line breeding of immunocompetent (regRG-KO) or (regFRG-KO) swine for rapid propagation. The rescue cassette can be excised, specifically in germ cells of regRG-KO or regFRG-KO swine, such that offspring of animals do not possess the rescue cassette and are immunodeficient. The immunodeficient swine also provide host embryos having genetic ablations to provide a niche for organ complementation by human stem cells.Type: ApplicationFiled: June 23, 2020Publication date: February 11, 2021Inventors: Daniel F. Carlson, Cheryl Lancto, Colin Fairman
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Patent number: 10893667Abstract: Methods, uses, and compositions for manipulating genomic DNA. Some of the embodiments of the invention provide for making a founder animal that is completely free of all unplanned genetic modifications. Some embodiments are directed to removing genetic faults in established breeds without making other alterations to the genome. Other embodiments are directed to particular tools or processes such as TALENs or CRISPR with a preferred truncation. One embodiment involves introducing a targeted targeting endonuclease system and a HDR template into a cell (optionally with a mismatch in the binding of the targeting endonuclease and the targeted site). Another embodiment includes processes of making a genetically modified livestock animal comprising a genome that comprises inactivation of a neuroendocrine gene selective for sexual maturation, with the inactivation of the gene preventing the animal from becoming sexually mature.Type: GrantFiled: September 11, 2019Date of Patent: January 19, 2021Assignee: Recombinetics, Inc.Inventors: Scott C. Fahrenkrug, Daniel F. Carlson
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Publication number: 20200407755Abstract: Methods and compositions for introducing genetic mutations into non-human animal cells are provided. These cells can be used to produce animal models of human disease. In some embodiments, the genetic mutations are flanked by DNA sequences that are “humanized” to match homologous DNA sequences. In some embodiments, the animal model is a large mammalian model for an inherited metabolic disorder. In some embodiments, the animal model is a pig model for phenylketonuria (PKU) created by introducing a missense mutation into exon 8 of the Pah gene.Type: ApplicationFiled: April 30, 2020Publication date: December 31, 2020Inventors: Joseph Lillegard, Daniel F. Carlson, Cary O. Harding, Dennis Webster
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Publication number: 20200017878Abstract: Methods, uses, and compositions for manipulating genomic DNA. Some of the embodiments of the invention provide for making a founder animal that is completely free of all unplanned genetic modifications. Some embodiments are directed to removing genetic faults in established breeds without making other alterations to the genome. Other embodiments are directed to particular tools or processes such as TALENs or CRISPR with a preferred truncation. One embodiment involves introducing a targeted targeting endonuclease system and a HDR template into a cell (optionally with a mismatch in the binding of the targeting endonuclease and the targeted site). Another embodiment includes processes of making a genetically modified livestock animal comprising a genome that comprises inactivation of a neuroendocrine gene selective for sexual maturation, with the inactivation of the gene preventing the animal from becoming sexually mature.Type: ApplicationFiled: September 11, 2019Publication date: January 16, 2020Inventors: Scott C. Fahrenkrug, Daniel F. Carlson
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Publication number: 20200017882Abstract: Human or humanized tissues and organs suitable for transplant are disclosed herein. Gene editing of a host animal provides a niche for complementation of the missing genetic information by donor stem cells. Editing of a host genome to knock out or disrupt genes responsible for the growth and/or differentiation of a target organ and injecting that animal at an embryo stage with donor stem cells to complement the missing genetic information for the growth and development of the organ. The result is a chimeric animal in which the complemented tissue (human/humanized organ) matches the genotype and phenotype of the donor. Such organs may be made in a single generation and the stem cell may be taken or generated from the patient's own body. As disclosed herein, it is possible to do so by simultaneously editing multiple genes in a cell or embryo creating a “niche” for the complemented tissue.Type: ApplicationFiled: April 16, 2019Publication date: January 16, 2020Inventors: Scott C. Fahrenkrug, Laurence Cooper, Perry B. Hackett, Daniel F. Carlson
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Publication number: 20200017877Abstract: Methods, uses, and compositions for manipulating genomic DNA. Some of the embodiments of the invention provide for making a founder animal that is completely free of all unplanned genetic modifications. Some embodiments are directed to removing genetic faults in established breeds without making other alterations to the genome. Other embodiments are directed to particular tools or processes such as TALENs or CRISPR with a preferred truncation. One embodiment involves introducing a targeted targeting endonuclease system and a HDR template into a cell (optionally with a mismatch in the binding of the targeting endonuclease and the targeted site). Another embodiment includes processes of making a genetically modified livestock animal comprising a genome that comprises inactivation of a neuroendocrine gene selective for sexual maturation, with the inactivation of the gene preventing the animal from becoming sexually mature.Type: ApplicationFiled: September 11, 2019Publication date: January 16, 2020Inventors: Scott C. Fahrenkrug, Daniel F. Carlson
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Publication number: 20190335725Abstract: A genetically modified livestock animal, and methods of making and using the same, the animal comprising a genetic modification to disrupt a target gene selectively involved in gametogenesis, wherein the disruption of the target gene prevents formation of functional gametes of the animal. Animals that create progeny with donor genetics, and methods of making and using the same. Cells, and methods of making and using the cells, with a genetic modification to disrupt a target gene selectively involved in gametogenesis.Type: ApplicationFiled: June 3, 2019Publication date: November 7, 2019Inventors: Scott C. Fahrenkrug, Daniel F. Carlson
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Publication number: 20190323031Abstract: Methods, uses, and compositions for manipulating genomic DNA. Some of the embodiments of the invention provide for making a founder animal that is completely free of all unplanned genetic modifications. Some embodiments are directed to removing genetic faults in established breeds without making other alterations to the genome. Other embodiments are directed to particular tools or processes such as TALENs or CRISPR with a preferred truncation. One embodiment involves introducing a targeted targeting endonuclease system and a HDR template into a cell (optionally with a mismatch in the binding of the targeting endonuclease and the targeted site). Another embodiment includes processes of making a genetically modified livestock animal comprising a genome that comprises inactivation of a neuroendocrine gene selective for sexual maturation, with the inactivation of the gene preventing the animal from becoming sexually mature.Type: ApplicationFiled: May 28, 2019Publication date: October 24, 2019Inventors: Scott C. Fahrenkrug, Daniel F. Carlson
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Publication number: 20190254266Abstract: Human or humanized tissues and organs suitable for transplant are disclosed herein. Gene editing of a host animal provides a niche for complementation of the missing genetic information by donor stem cells. Editing of a host genome to knock out or debilitate genes responsible for the growth and/or differentiation of a target organ and injecting that animal at an embryo stage with donor stem cells to complement the missing genetic information for the growth and development of the organ. The result is a chimeric animal in which the complemented tissue (human/humanized organ) matches the genotype and phenotype of the donor. Such organs may be made in a single generation and the stem cell may be taken or generated from the patient's own body. As disclosed herein, it is possible to do so by simultaneously editing multiple genes in a cell or embryo creating a “niche” for the complemented tissue.Type: ApplicationFiled: October 27, 2016Publication date: August 22, 2019Applicants: Regents of the University of Minnesota, Recombinetics, Inc., Board of Regents of the University of Texas SystemInventors: Scott C. Fahrenkrug, Daniel F. Carlson, Peter Igarashi, Thomas Carroll
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Publication number: 20190223417Abstract: Disclosed herein are genomically modified livestock animals and methods to provide them that express the SLICK phenotype. The animals disclosed herein express a truncated allele for the prolactin receptor (PRLR) gene. When expressed, the livestock animals produce a PRLR that is missing up to the terminal 148 amino acid (aa) residues of the protein all ranges and values within the explicitly stated range are contemplated: e.g., from 148 to 69. Animals expressing SLICK have superior thermoregulatory ability compared to non-slick animals and experience a less drastic depression in milk yield during the summer.Type: ApplicationFiled: February 21, 2019Publication date: July 25, 2019Inventors: Tad S. Sonstegard, Daniel F. Carlson, Scott C. Fahrenkrug
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Publication number: 20190194687Abstract: Methods, uses, and animals for introgression of alleles between animals, including SNPs. One embodiment involves introducing a targeted targeting endonuclease system and a HDR template into a cell with a mismatch in the binding of the targeting endonuclease and the targeted site.Type: ApplicationFiled: March 6, 2019Publication date: June 27, 2019Inventors: Scott C. Fahrenkrug, Daniel F. Carlson, Tad S. Sonstegard