Patents by Inventor Daniel George Fordham
Daniel George Fordham has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20240076719Abstract: A method for determining the presence, absence or amount of two or more target polynucleotides in a sample comprising additional components, the method comprising: (i) contacting the sample with a panel of two or more probes under conditions suitable for hybridisation of the target polynucleotides to the probes, wherein: (a) each probe comprises a non-hybridisation region and a hybridisation region that specifically hybridises to one of the target polynucleotides to form a hybridised probe; and (b) the hybridisation region of a probe of the panel comprises one or more non-natural nucleotides; (ii) contacting the sample prepared in step (i) with a transmembrane pore through which a single stranded polynucleotide but not a double stranded polynucleotide can pass and applying a potential difference to the transmembrane pore such that the hybridised probes in the sample interact with the pore; (iii) measuring current blockades having a duration within a defined window, wherein: (a) the one or more non-natuType: ApplicationFiled: January 27, 2023Publication date: March 7, 2024Applicant: Oxford Nanopore Technologies PLCInventors: Nicholas Antony Smith, Daniel John Turner, Daniel George Fordham, James White
-
Publication number: 20240060969Abstract: The invention relates to a method of determining the presence or absence of a target analyte in a sample. The method comprises immobilising any target analyte present in the sample on a surface; contacting the surface with: (i) a first detection agent that binds specifically to the target analyte; and (ii) a reporter polynucleotide, wherein the reporter polynucleotide is bound to, or binds to, the first detection agent; and contacting a transmembrane pore with any reporter polynucleotide that has been immobilised on the surface, wherein the reporter polynucleotide is immobilised on the surface by binding of the first agent to the target analyte, and using the transmembrane pore to detect the reporter polynucleotide, thereby determining the presence or absence of the target analyte in the sample.Type: ApplicationFiled: August 24, 2023Publication date: February 22, 2024Applicant: Oxford Nanopore Technologies PLCInventor: Daniel George Fordham
-
Publication number: 20230392157Abstract: The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.Type: ApplicationFiled: May 12, 2023Publication date: December 7, 2023Applicant: Oxford Nanopore Technologies PLCInventors: Daniel John Turner, Daniel George Fordham, Roger Charles Gill, Clive Gavin Brown, Stuart Reid, James Anthony Clarke, James White
-
Patent number: 11789014Abstract: The invention relates to a method of determining the presence or absence of a target analyte in a sample. The method comprises immobilising any target analyte present in the sample on a surface; contacting the surface with: (i) a first detection agent that binds specifically to the target analyte; and (ii) a reporter polynucleotide, wherein the reporter polynucleotide is bound to, or binds to, the first detection agent; and contacting a transmembrane pore with any reporter polynucleotide that has been immobilised on the surface, wherein the reporter polynucleotide is immobilised on the surface by binding of the first agent to the target analyte, and using the transmembrane pore to detect the reporter polynucleotide, thereby determining the presence or absence of the target analyte in the sample.Type: GrantFiled: May 3, 2018Date of Patent: October 17, 2023Assignee: Oxford Nanopore Technologies PLCInventor: Daniel George Fordham
-
Publication number: 20230295694Abstract: A method for determining the presence, absence or amount of two or more target polynucleotides in a sample comprising additional components, the method comprising: (i) contacting the sample with a panel of two or more probes under conditions suitable for hybridisation of the target polynucleotides to the probes, wherein: (a) each probe comprises a non-hybridisation region and a hybridisation region that specifically hybridises to one of the target polynucleotides to form a hybridised probe; and (b) the hybridisation region of a probe of the panel comprises one or more non-natural nucleotides; (ii) contacting the sample prepared in step (i) with a transmembrane pore through which a single stranded polynucleotide but not a double stranded polynucleotide can pass and applying a potential difference to the transmembrane pore such that the hybridised probes in the sample interact with the pore; (iii) measuring current blockades having a duration within a defined window, wherein: (a) the one or more non-natuType: ApplicationFiled: January 27, 2023Publication date: September 21, 2023Applicant: Oxford Nanopore Technologies PLCInventors: Nicholas Antony Smith, Daniel John Turner, Daniel George Fordham, James White
-
Patent number: 11739379Abstract: The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.Type: GrantFiled: June 29, 2021Date of Patent: August 29, 2023Assignee: Oxford Nanopore Technologies PLCInventors: Andrew John Heron, James Edward Graham, Richard Alexander Gutierrez, Rebecca Victoria Bowen, James White, Clive Gavin Brown, Daniel George Fordham
-
Patent number: 11685922Abstract: The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.Type: GrantFiled: June 30, 2020Date of Patent: June 27, 2023Assignee: Oxford Nanopore Technologies PLCInventors: Daniel John Turner, Daniel George Fordham, Roger Charles Gill, Clive Gavin Brown, Stuart Reid, James Anthony Clarke, James White
-
Patent number: 11390910Abstract: A method for determining the presence, absence or amount of two or more target polynucleotides in a sample comprising additional components, the method comprising: (i) contacting the sample with a panel of two or more probes under conditions suitable for hybridisation of the target polynucleotides to the probes, wherein: (a) each probe comprises a non-hybridisation region and a hybridisation region that specifically hybridises to one of the target polynucleotides to form a hybridised probe; and (b) the hybridisation region of a probe of the panel comprises one or more non-natural nucleotides; (ii) contacting the sample prepared in step (i) with a transmembrane pore through which a single stranded polynucleotide but not a double stranded polynucleotide can pass and applying a potential difference to the transmembrane pore such that the hybridised probes in the sample interact with the pore; (iii) measuring current blockades having a duration within a defined window, wherein: (a) the one or more non-natural nucType: GrantFiled: October 20, 2017Date of Patent: July 19, 2022Assignee: Oxford Nanopore Technologies PLCInventors: Nicholas Antony Smith, Daniel John Turner, Daniel George Fordham, James White
-
Publication number: 20220064723Abstract: The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.Type: ApplicationFiled: June 29, 2021Publication date: March 3, 2022Applicant: Oxford Nanopore Technologies LimitedInventors: Andrew John HERON, James Edward GRAHAM, Richard Alexander GUTIERREZ, Rebecca Victoria BOWEN, James WHITE, Clive Gavin BROWN, Daniel George FORDHAM
-
Patent number: 11085078Abstract: The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.Type: GrantFiled: September 29, 2017Date of Patent: August 10, 2021Assignee: Oxford Nanopore Technologies LimitedInventors: Andrew John Heron, James Edward Graham, Richard Alexander Gutierrez, Rebecca Victoria Bowen, James White, Clive Gavin Brown, Daniel George Fordham
-
Publication number: 20210198656Abstract: The invention provides methods of selecting polynucleotides based on their size in globular form. The polynucleotides may be treated with a condensation medium, e.g. comprising polyethylene glycol, e.g. PEG8000, and salt, in order to adopt a globular form. The method may employing filtering in order to retain the larger nucleic acids and make the larger nucleic acids available for sequencing applications, such as nanopore sequencing. The invention also relates to kits for selecting polynucleotides.Type: ApplicationFiled: May 24, 2019Publication date: July 1, 2021Applicant: Oxford Nanopore Technologies LimitedInventor: Daniel George Fordham
-
Publication number: 20210198718Abstract: A method of attaching an adapter to a polynucleotide comprising: providing a double stranded polynucleotide comprising a single stranded break point within its polynucleotide sequence; contacting said double stranded polynucleotide with an exonuclease to form a single stranded region initiated at said break point; attaching an adapter to said single stranded region.Type: ApplicationFiled: May 24, 2019Publication date: July 1, 2021Applicant: Oxford Nanopore Technologies LimitedInventors: Daniel George Fordham, Phillip Laurence James
-
Publication number: 20210148903Abstract: The invention relates to a method of determining the presence or absence of a target analyte in a sample. The method comprises immobilising any target analyte present in the sample on a surface; contacting the surface with: (i) a first detection agent that binds specifically to the target analyte; and (ii) a reporter polynucleotide, wherein the reporter polynucleotide is bound to, or binds to, the first detection agent; and contacting a transmembrane pore with any reporter polynucleotide that has been immobilised on the surface, wherein the reporter polynucleotide is immobilised on the surface by binding of the first agent to the target analyte, and using the transmembrane pore to detect the reporter polynucleotide, thereby determining the presence or absence of the target analyte in the sample.Type: ApplicationFiled: May 3, 2018Publication date: May 20, 2021Applicant: Oxford Nanopore Technologies LimitedInventor: Daniel George Fordham
-
Publication number: 20210018500Abstract: The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.Type: ApplicationFiled: June 30, 2020Publication date: January 21, 2021Applicant: Oxford Nanopore Technologies LimitedInventors: Daniel John Turner, Daniel George Fordham, Roger Charles Gill, Clive Gavin Brown, Stuart Reid, James Anthony Clarke, James White
-
Patent number: 10739341Abstract: The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.Type: GrantFiled: February 14, 2013Date of Patent: August 11, 2020Assignee: Oxford Nanopore Technologies LimitedInventors: Daniel John Turner, Daniel George Fordham, Roger Charles Gill, Clive Gavin Brown, Stuart Reid, James Anthony Clarke, James White
-
Publication number: 20200024654Abstract: The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.Type: ApplicationFiled: September 29, 2017Publication date: January 23, 2020Applicant: Oxford Nanopore Technologies LimitedInventors: Andrew John Heron, James Edward Graham, Richard Alexander Gutierrez, Rebecca Victoria Bowen, James White, Clive Gavin Brown, Daniel George Fordham
-
Publication number: 20190249231Abstract: A method for determining the presence, absence or amount of two or more target polynucleotides in a sample comprising additional components, the method comprising: (i) contacting the sample with a panel of two or more probes under conditions suitable for hybridisation of the target polynucleotides to the probes, wherein: (a) each probe comprises a non-hybridisation region and a hybridisation region that specifically hybridises to one of the target polynucleotides to form a hybridised probe; and (b) the hybridisation region of a probe of the panel comprises one or more non-natural nucleotides; (ii) contacting the sample prepared in step (i) with a transmembrane pore through which a single stranded polynucleotide but not a double stranded polynucleotide can pass and applying a potential difference to the transmembrane pore such that the hybridised probes in the sample interact with the pore; (iii) measuring current blockades having a duration within a defined window, wherein: (a) the one or more non-natural nucType: ApplicationFiled: October 20, 2017Publication date: August 15, 2019Applicant: Oxford Nanopore Technologies LimitedInventors: Nicholas Antony Smith, Daniel John Turner, Daniel George Fordham, James White
-
Patent number: 9995728Abstract: The invention relates to a new method of determining in a sample the presence, absence or concentration of one or more target analytes, such as micro-ribonucleic acids (microRNAs or miRNAs). The invention may therefore relate to a multiplex assay for determining the presence or absence of each target analyte in a group of multiple analytess. The invention uses one or more probes comprising a quadruplex and transmembrane pores.Type: GrantFiled: November 6, 2013Date of Patent: June 12, 2018Assignee: Oxford Nanopore Technologies Ltd.Inventors: Daniel George Fordham, Nicholas Smith, Daniel John Turner
-
Publication number: 20150301015Abstract: The invention relates to a new method of determining in a sample the presence, absence or concentration of one or more target analytes, such as micro-ribonucleic acids (microRNAs or miRNAs). The invention may therefore relate to a multiplex assay for determining the presence or absence of each target analyte in a group of multiple analytess. The invention uses one or more probes comprising a quadruplex and transmembrane pores.Type: ApplicationFiled: November 6, 2013Publication date: October 22, 2015Inventors: Daniel George Fordham, Nicholas Smith, Daniel John Turner
-
Publication number: 20150177237Abstract: The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.Type: ApplicationFiled: February 14, 2013Publication date: June 25, 2015Inventors: Daniel John Turner, Daniel George Fordham, Roger Charles Gill, Clive Gavin Brown, Stuart Reid, James Anthony Clarke, James White