Patents by Inventor Daniel Joseph Macool
Daniel Joseph Macool has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20210395756Abstract: Described are compositions and methods relating to modified yeast that over-express the ribonucleotide reductase inhibitor, HUG1. The yeast produces an increased amount of alcohol compared to parental cells. Such yeast is particularly useful for large-scale ethanol production from starch substrates where acetate in an undesirable end product.Type: ApplicationFiled: September 19, 2019Publication date: December 23, 2021Inventors: Daniel Joseph MACOOL, Quinn Qun ZHU
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Publication number: 20210221857Abstract: Described are compositions and methods relating to modified yeast cells that over-expresses the transcriptional activator/repressor, GIS1. The yeast cells produce an increased amount of ethanol compared to their parental cells.Type: ApplicationFiled: May 21, 2019Publication date: July 22, 2021Inventors: Daniel Joseph MACOOL, Paula Johanna Maria TEUNISSEN, Quinn Qun ZHU
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Publication number: 20200377559Abstract: Described are compositions and methods relating to the over-expression of sugar transporter-like polypeptides to reduce the amount of glycerol and acetate produced by modified yeast having an exogenous pathway that cause it to produce more ethanol and acetate than its parental yeast.Type: ApplicationFiled: March 24, 2018Publication date: December 3, 2020Inventors: Daniel Joseph Macool, Paula Johanna Maria Teunissen, Yehong Jamie Wang, Hyeryoung Yoon, Quinn Qun Zhu
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Patent number: 8916365Abstract: Transgenic Yarrowia species are disclosed herein that comprise a polynucleotide encoding a cytosolic malic enzyme, a lipid content that is at least about 35% by weight of the dry cell weight of the Yarrowia species, and an engineered polyunsaturated fatty acid (PUFA) biosynthetic pathway, wherein overexpression of the cytosolic malic enzyme increases lipid content.Type: GrantFiled: March 14, 2013Date of Patent: December 23, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Daniel Joseph Macool, Zhixiong Xue, Quinn Qun Zhu
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Patent number: 8815566Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ?-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.Type: GrantFiled: July 17, 2013Date of Patent: August 26, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Howard Glenn Damude, Peter John Gillies, Daniel Joseph Macool, Stephen K Picataggio, Dana M. Walters Pollak, James John Ragghianti, Zhixiong Xue, Narendra S Yadav, Hongxiang Zhang, Quinn Qun Zhu
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Patent number: 8685682Abstract: An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.Type: GrantFiled: May 14, 2009Date of Patent: April 1, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Howard Glenn Damude, Peter John Gillies, Daniel Joseph Macool, Stephen K Picataggio, James John Ragghianti, John E Seip, Zhixiong Xue, Narendra S Yadav, Hongxiang Zhang, Quinn Qun Zhu
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Publication number: 20140030772Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ?-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.Type: ApplicationFiled: July 17, 2013Publication date: January 30, 2014Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: Howard Glenn Damude, Peter John Gillies, Daniel Joseph Macool, Stephen K. Picataggio, Dana M. Walters Pollak, James John Ragghianti, Zhixiong Xue, Narendra S. Yadav, Hongxiang Zhang, Quinn Qun Zhu
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Publication number: 20130260427Abstract: Transgenic Yarrowia species are disclosed herein that comprise a polynucleotide encoding a cytosolic malic enzyme, a lipid content that is at least about 35% by weight of the dry cell weight of the Yarrowia species, and an engineered polyunsaturated fatty acid (PUFA) biosynthetic pathway, wherein overexpression of the cytosolic malic enzyme increases lipid content.Type: ApplicationFiled: March 14, 2013Publication date: October 3, 2013Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: Daniel Joseph Macool, Zhixiong Xue, Quinn Qun Zhu
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Patent number: 8518674Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ?-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.Type: GrantFiled: September 29, 2010Date of Patent: August 27, 2013Assignee: E I du Pont de Nemours and CompanyInventors: Howard Glenn Damude, Peter John Gillies, Daniel Joseph Macool, Stephen K. Picataggio, Dana M. Walters Pollak, James John Ragghianti, Zhixiong Xue, Narendra S. Yadav, Hongxiang Zhang, Quinn Qun Zhu
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Patent number: 7932077Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ?-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.Type: GrantFiled: November 2, 2005Date of Patent: April 26, 2011Assignee: E. I. du Pont de Nemours and CompanyInventors: Howard Glenn Damude, Peter John Gillies, Daniel Joseph Macool, Stephen K. Picataggio, Dana M. Walters Pollak, James John Ragghianti, Zhixiong Xue, Narendra S. Yadav, Hongxiang Zhang, Quinn Qun Zhu
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Publication number: 20110086919Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ?-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.Type: ApplicationFiled: September 29, 2010Publication date: April 14, 2011Applicant: E. I. Du Pont De Nemours and CompanyInventors: Howard Glenn Damude, Peter John Gillies, Daniel Joseph Macool, Stephen K. Picataggio, Dana M. Walters Pollak, James John Ragghianti, Zhixiong Xue, Narendra S. Yadav, Hongxiang Zhang, Quinn Qun Zhu
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Publication number: 20100022647Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 10% arachidonic acid (ARA, an ?-6 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases, and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of ARA. Production host cells are claimed, as are methods for producing ARA within said host cells.Type: ApplicationFiled: July 30, 2009Publication date: January 28, 2010Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventors: Howard Glenn Damude, Peter John Gillies, Daniel Joseph Macool, Stephen K. Picataggio, Dana M. Walters Pollak, James John Ragghianti, Zhixiong Xue, Narendra S. Yadav, Hongxiang Zhang, Quinn Qun Zhu
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Publication number: 20090311380Abstract: An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.Type: ApplicationFiled: May 14, 2009Publication date: December 17, 2009Applicant: E.I. DU PONT DE NEMOURS AND COMPANYInventors: Howard Glenn Damude, Peter John Gillies, Daniel Joseph Macool, Stephen K. Picataggio, James John Ragghianti, John E. Seip, Zhixiong Xue, Narendra S. Yadav, Hongxiang Zhang, Quinn Qun Zhu
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Patent number: 7588931Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 10% arachidonic acid (ARA, an ?-6 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases, and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of ARA. Production host cells are claimed, as are methods for producing ARA within said host cells.Type: GrantFiled: November 1, 2005Date of Patent: September 15, 2009Assignee: E. I. du Pont de Nemours and CompanyInventors: Howard Glenn Damude, Peter John Gillies, Daniel Joseph Macool, Stephen K. Picataggio, Dana M. Walters Pollak, James John Ragghianti, Zhixiong Xue, Narendra S. Yadav, Hongxiang Zhang, Quinn Qun Zhu
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Patent number: 7550286Abstract: An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.Type: GrantFiled: November 1, 2005Date of Patent: June 23, 2009Assignee: E. I. du Pont de Nemours and CompanyInventors: Howard Glenn Damude, Daniel Joseph Macool, Stephen K. Picataggio, James John Ragghianti, John E. Seip, Zhixiong Xue, Narendra S. Yadav, Hongxiang Zhang, Quinn Qun Zhu
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Patent number: 7470532Abstract: The present invention relates to a fungal C16/18 fatty acid elongase that is able to catalyze the conversion of palmitate (16:0) to stearic acid (18:0). Specifically, the nucleotide sequence of a Mortierella alpina C16/18 fatty acid elongase is provided (designated as “ELO3”). Methods of increasing microbial oil production, increasing carbon flux into the polyunsaturated fatty acid biosynthetic pathway and increasing the content of polyunsaturated fatty acids by over-expression of the C16/18 fatty acid elongase are described herein. Most desirably, the substrate specificity of the instant ELO3 will be particularly useful to enable accumulation of long-chain polyunsaturated fatty acids in oleaginous yeast, such as Yarrowia lipolytica.Type: GrantFiled: October 19, 2005Date of Patent: December 30, 2008Assignee: E.I. du Pont de Nemours and CompanyInventors: Daniel Joseph Macool, Zhixiong Xue, Quinn Qun Zhu
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Patent number: 7198937Abstract: An acyltransferase is provided, suitable for use in the manufacture of microbial oils enriched in omega fatty acids in oleaginous organisms. Specifically, the gene encoding diacylglycerol acyltransferase (DGAT2) has been isolated from Mortierella alpina. This gene encodes an enzyme that participates in the terminal step in oil biosynthesis in fungi and yeast and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids produced in oils of oleaginous organisms. Most desirably, the substrate specificity of the instant DGAT2 will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.Type: GrantFiled: December 29, 2004Date of Patent: April 3, 2007Assignee: E. I. du Pont de Nemours and CompanyInventors: Zhixiong Xue, Narendra S. Yadav, Daniel Joseph Macool
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Patent number: 7192762Abstract: Glycerol-3-phosphate o-acyltransferase (GPAT) participates in the first step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids (PUFAs) produced in oils of oleaginous organisms. The present application provides a nucleic acid fragment isolated from Mortierella alpina encoding a GPAT that is suitable for use in the manufacture of oils enriched in omega fatty acids in oleaginous organisms. Most desirably, the substrate specificity of the instant GPAT will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.Type: GrantFiled: October 19, 2005Date of Patent: March 20, 2007Assignee: E. I. du Pont de Nemours and CompanyInventors: Daniel Joseph Macool, Zhixiong Xue