Abstract: A bubble trap having a cylindrically symmetrical chamber, a filter covering an outlet and offset from the axis of the chamber, an inlet with a plurality of vertically aligned orifices, the orifices arranged to produce circumferential flow within the chamber. Bubbles in the liquid coalesce on the filter and are removed. A method of removing bubbles is also disclosed.

Abstract: A bubble trap having a cylindrically symmetrical chamber, a filter covering an outlet and offset from the axis of the chamber, an inlet with a plurality of vertically aligned orifices, the orifices arranged to produce circumferential flow within the chamber. Bubbles in the liquid coalesce on the filter and are removed. A method of removing bubbles is also disclosed.

Abstract: In a method and apparatus for the submersed growth of human and animal cell cultures, the cell growth container is provided with a centrally disposed permeator, which is formed simultaneously as conduit tube and reactor, and is provided on both sides with gas-permeable membranes. A gas of defined composition is led through the hollow porous support body of the permeator as fresh gas on one side, and led away on the other side as waste gas. In this manner the gas delivers oxygen across the membrane and receives carbon dioxide. The nutrient substrate and the tissue cells are held in suspension by means of a stirrer provided in the interior of the permeator. Foam problems do not occur, since no gas bubbles can enter into the medium.

Abstract: In submerged growing of tissue cells on microcarriers in a culture medium, the culture medium is alternately aerated or withdrawn from a bottom of a culture container via a sintered plate. An air chamber is formed under the sintered plate.