Patents by Inventor Daniel Mazur
Daniel Mazur has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240117411Abstract: Methods of controlling the density or number of nucleic acid molecules coupled to solid supports are provided. Capture substrates produced thereby or comprising a predetermined density or number of nucleic acids coupled thereto are also provided.Type: ApplicationFiled: December 14, 2023Publication date: April 11, 2024Inventors: Robert ONO, Daniel MAZUR, Florian OBERSTRASS
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Publication number: 20240101980Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification.Type: ApplicationFiled: November 28, 2023Publication date: March 28, 2024Inventors: Daniel MAZUR, Sihong CHEN, Peter VANDER HORN, Eileen TOZER, Guobin LUO, Joshua SHIRLEY, Kevin HEINEMANN
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Publication number: 20240067941Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.Type: ApplicationFiled: October 13, 2023Publication date: February 29, 2024Inventors: Peter VANDER HORN, Theo NIKIFOROV, Daniel MAZUR, Eileen TOZER, Mindy LANDES
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Publication number: 20240043833Abstract: Provided herein are methods, systems, compositions, and kits that can determine spatial information between a plurality of analytes in a sample by tagging such analytes with tags, where identities and/or locations of such tags are previously unknown prior to tagging. Each of a plurality of analyte sequences may be tagged with multiple spatial tags, such that each analyte sequence is associated with a set of two or more spatial tags. The sets of spatial tags may be analyzed to generate a map of analyte sequences. The map may comprise information about the respective absolute positions of each of a set of sequences with respect to a reference sequence. The map may comprise information about the respective probability cloud (or likely location) of each of a set of sequences with respect to a reference sequence.Type: ApplicationFiled: October 17, 2023Publication date: February 8, 2024Inventors: Florian OBERSTRASS, Ron Saar DOVER, Zohar SHIPONY, Gilad ALMOGY, Daniel MAZUR
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Patent number: 11866740Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification.Type: GrantFiled: May 7, 2021Date of Patent: January 9, 2024Assignee: Life Technologies CorporationInventors: Daniel Mazur, Peter B. Vander Horn, Eileen Tozer, Sihong Chen, Guobin Luo, Joshua Shirley, Kevin Heinemann
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Patent number: 11821011Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.Type: GrantFiled: August 13, 2021Date of Patent: November 21, 2023Assignee: Life Technologies CorporationInventors: Peter Vander Horn, Daniel Mazur, Theo Nikiforov, Mindy Landes, Eileen Tozer
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Publication number: 20230340570Abstract: The present disclosure provides methods, systems, compositions, and kits for reducing particle aggregation and improving sequencing quality. A method for processing particles may comprise the use of a first buffer solution or a second buffer solution. A method for processing particles may comprise the use of a single-strand binding moiety such as a single-strand binding protein.Type: ApplicationFiled: February 24, 2023Publication date: October 26, 2023Inventors: Daniel MAZUR, Kevin HEINEMANN, Theo NIKIFOROV, Robert ONO, Aklilu WORKU, Florian OBERSTRASS
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Publication number: 20230332226Abstract: The present disclosure provides compositions, methods, and systems for enrichment and analysis of template nucleic acid molecules, e.g., of a biological sample. Further provided herein are methods of using a plurality of unique bead species each comprising a unique set of identical primer molecules.Type: ApplicationFiled: February 28, 2023Publication date: October 19, 2023Inventors: Florian OBERSTRASS, Theo NIKIFOROV, Mark STAPLETON, Daniel MAZUR, Gilad ALMOGY, Ishminder MANN, Chandan SHEE, Benjamin SCHROEDER
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Publication number: 20230062391Abstract: The present disclosure provides compositions comprising nucleic acid molecules coupled to supports and comprising one or more cleavable or excisable moieties. Methods of enriching and sequencing nucleic acid molecules are also provided.Type: ApplicationFiled: July 25, 2022Publication date: March 2, 2023Inventors: Daniel MAZUR, Florian OBERSTRASS
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Publication number: 20230055565Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.Type: ApplicationFiled: September 26, 2022Publication date: February 23, 2023Applicant: Life Technologies CorporationInventors: Stephen P. Hendricks, Michael Phelan, Marian Peris, Cheng-Yao Chen, Daniel Mazur, Xinzhan Peng, Amy Castillo
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Publication number: 20220372472Abstract: The present invention relates to a method for generating a library of different polynucleotide molecules, by ligating a double-stranded polynucleotide to a plurality of different target polynucleotide duplexes, the double-stranded polynucleotide comprising: (a) a first strand comprising an annealed portion and an overhang portion; and (b) a second strand consisting essentially of an annealed portion, wherein the second strand is complementary to and annealed to the annealed portion of the first strand.Type: ApplicationFiled: November 5, 2020Publication date: November 24, 2022Inventors: Florian OBERSTRASS, Daniel MAZUR
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Publication number: 20220315917Abstract: Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.Type: ApplicationFiled: May 10, 2021Publication date: October 6, 2022Inventors: Mark ANDERSEN, Daniel MAZUR, Sihong CHEN, Guobin LUO, Xinzhan PENG
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Publication number: 20220307071Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a referenceTaq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.Type: ApplicationFiled: April 20, 2022Publication date: September 29, 2022Applicant: Life Technologies CorporationInventors: Daniel Mazur, Eileen Tozer, Sihong Chen, Peter Vander Horn, Tommie Lincecum
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Patent number: 11453909Abstract: Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.Type: GrantFiled: September 6, 2018Date of Patent: September 27, 2022Assignee: Life Technologies CorporationInventors: Stephen P. Hendricks, Michael Phelan, Marian Peris, Cheng-Yao Chen, Daniel Mazur, Xinzhan Peng, Amy Castillo
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Publication number: 20220162573Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.Type: ApplicationFiled: December 20, 2021Publication date: May 26, 2022Inventors: Peter VANDER HORN, Theo NIKIFOROV, Guobin LUO, Mindy LANDES, Daniel MAZUR, Eileen TOZER, Tommie LINCECUM
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Patent number: 11319584Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof, such as modified Taq polymerases, are provided that allow for improved nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having improved thermostability, accuracy, processivity and/or read length as compared to a referenceTaq polymerase. In some aspects, the disclosure relates to modified polymerases or biologically active fragments thereof, useful for amplification methods, and in practically illustrative embodiments, emulsion PCR.Type: GrantFiled: August 14, 2020Date of Patent: May 3, 2022Assignee: Life Technologies CorporationInventors: Daniel Mazur, Eileen Tozer, Sihong Chen, Peter Vander Horn, Tommie Lincecum
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Patent number: 11208636Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.Type: GrantFiled: April 16, 2020Date of Patent: December 28, 2021Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Peter Vander Horn, Theo Nikiforov, Guobin Luo, Mindy Landes, Daniel Mazur, Eileen Tozer, Tommie Lincecum
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Publication number: 20210371834Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.Type: ApplicationFiled: August 13, 2021Publication date: December 2, 2021Inventors: Peter VANDER HORN, Daniel MAZUR, Theo NIKIFOROV, Mindy LANDES, Eileen TOZER
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Publication number: 20210284976Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides recombinant polymerases that yield lower systematic error rates and/or improved accuracy, when used in sequencing by synthesis reactions as compared to a control polymerase. In one aspect, the disclosure relates to recombinant polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In another aspect, the recombinant polymerases are useful for the amplification of nucleic acid templates during PCR, emPCR, isothermal amplification, recombinase polymerase amplification, rolling circle amplification, strand displacement amplification and proximity ligation amplification.Type: ApplicationFiled: May 7, 2021Publication date: September 16, 2021Inventors: Daniel MAZUR, Peter B. VANDER HORN, Eileen TOZER, Sihong CHEN, Guobin LUO, Joshua SHIRLEY, Kevin HEINEMANN
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Patent number: 11091746Abstract: The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.Type: GrantFiled: June 5, 2020Date of Patent: August 17, 2021Assignee: Life Technologies CorporationInventors: Peter Vander Horn, Daniel Mazur, Theo Nikiforov, Mindy Landes, Eileen Tozer