Abstract: The enzymes of the ACC synthase family are used in producing ethylene. Nucleotide and polypeptide sequences of ACC synthases are provided along with knockout plant cells having inhibition in expression and/or activity in an ACC synthase and knockout plants displaying a staygreen phenotype, a male sterility phenotype, or an inhibition in ethylene production. Methods for modulating staygreen potential in plants, methods for modulating sterility in plants, and methods for inhibiting ethylene production in plants are also provided.

Abstract: The enzymes of the ACC synthase family are used in producing ethylene. Nucleotide and polypeptide sequences of ACC synthases are provided along with knockout plant cells having inhibition in expression and/or activity in an ACC synthase and knockout plants displaying a staygreen phenotype, a male sterility phenotype, or an inhibition in ethylene production. Methods for modulating staygreen potential in plants, methods for modulating sterility in plants, and methods for inhibiting ethylene production in plants are also provided.

Abstract: The present invention is directed to plant genetic engineering. In particular, it is directed to producing green leaves in maize through inhibition of ethylene. The genes involved in producing this phenotype include 1-Aminocyclopropane-1-Carboxylate (“ACC”) synthase, ACC oxidase, ACC deaminase, ethylene response sensor (“ERS”), ethylene resistant (“ETR”), and ethylene insensitive (“EIN”).

Abstract: The enzymes of the ACC synthase family are used in producing ethylene. Nucleotide and polypeptide sequences of ACC synthases are provided along with knockout plant cells having inhibition in expression and/or activity in an ACC synthase and knockout plants displaying a staygreen phenotype, a male sterility phenotype, or an inhibition in ethylene production. Methods for modulating staygreen potential in plants, methods for modulating sterility in plants, and methods for inhibiting ethylene production in plants are also provided.

Abstract: The enzymes of the ACC synthase family are used in producing ethylene. Nucleotide and polypeptide sequences of ACC synthases are provided along with knockout plant cells having inhibition in expression and/or activity in an ACC synthase and knockout plants displaying a staygreen phenotype, a male sterility phenotype, or an inhibition in ethylene production. Methods for modulating staygreen potential in plants, methods for modulating sterility in plants, and methods for inhibiting ethylene production in plants are also provided.

Abstract: A gene expression system for controllable expression of ethylene response in a plant cell includes an activation cassette comprising a DNA-binding domain that recognizes a response element; an ecdysone receptor ligand binding domain; and an activation domain; and a target cassette comprising an inducible promoter, which comprises, in operative association, the response element and a minimal promoter responsive to the activation domain. The inducible promoter controls the expression of a nucleic acid sequence that encodes a selected protein that modifies sensitivity to ethylene in the plant. Interaction among the components of the activation cassette and target cassette, when in a plant cell, in the presence of an inducing composition, modulates expression of the selected protein and selectively modulates ethylene sensitivity in the plant cell. This modulation in the expression of the protein is controlled by the timing, the concentration and the duration of the application of the inducing composition.

Abstract: The enzymes of the ACC synthase family are used in producing ethylene. Nucleotide and polypeptide sequences of ACC synthases, namely ACS2, ACS6, and ACS7 from Zea mays, are provided. Knockout plant cells having inhibition in expression and/or activity in an ACC synthase such as ACS2, ACS6, or ACS7 and knockout plants displaying a staygreen phenotype, a male sterility phenotype, or an inhibition in ethylene production are also provided, as are seeds obtained from such plants. Methods for modulating staygreen potential in plants, methods for modulating sterility in plants, and methods for inhibiting ethylene production in plants are also provided.

Abstract: The enzymes of the ACC synthase family are used in producing ethylene. Nucleotide and polypeptide sequences of ACC synthases, namely ACS2, ACS6, and ACS7 from Zea mays, are provided. Knockout plant cells having inhibition in expression and/or activity in an ACC synthase such as ACS2, ACS6, or ACS7 and knockout plants displaying a staygreen phenotype, a male sterility phenotype, or an inhibition in ethylene production are also provided, as are seeds obtained from such plants. Methods for modulating staygreen potential in plants, methods for modulating sterility in plants, and methods for inhibiting ethylene production in plants are also provided.

Abstract: The present invention is directed to plant genetic engineering. In particular, it is directed to producing green leaves and increasing productivity through inhibition of ethylene. The compositions and methods of the invention involve dominant negative ethylene receptors that interfere with ethylene signaling.

Abstract: The present invention provides new methods of delaying senescence in a plant by inhibiting ACC oxidase, or EIN2 activity in the plant. In particular, it is directed to producing green leaves in maize through inhibition of ethylene. The genes involved in producing this phenotype include ACC deaminase, ethylene response sensor (“ERS”), ethylene resistant (“ETR”), and ethylene insensitive (“EIN”). The delay in senescence can be achieved through the production of ACC deaminase, mutated ETR1 and ERS2 proteins, as well as overexpression of wild-type ETR1 and ERS2 proteins.

Abstract: The enzymes of the ACC synthase family are used in producing ethylene. Nucleotide and polypeptide sequences of ACC synthases are provided along with knockout plant cells having inhibition in expression and/or activity in an ACC synthase and knockout plants displaying a staygreen phenotype, a male sterility phenotype, or an inhibition in ethylene production. Methods for modulating staygreen potential in plants, methods for modulating sterility in plants, and methods for inhibiting ethylene production in plants are also provided.

Abstract: The enzymes of the ACC synthase family are used in producing ethylene. Nucleotide and polypeptide sequences of ACC synthases are provided along with knockout plant cells having inhibition in expression and/or activity in an ACC synthase and knockout plants displaying a staygreen phenotype, a male sterility phenotype, or an inhibition in ethylene production. Methods for modulating staygreen potential in plants, methods for modulating sterility in plants, and methods for inhibiting ethylene production in plants are also provided.

Abstract: The enzymes of the ACC synthase family are used in producing ethylene. Nucleotide and polypeptide sequences of ACC synthases are provided along with knockout plant cells having inhibition in expression and/or activity in an ACC synthase and knockout plants displaying a staygreen phenotype, a male sterility phenotype, or an inhibition in ethylene production. Methods for modulating staygreen potential in plants, methods for modulating sterility in plants, and methods for inhibiting ethylene production in plants are also provided.

Abstract: The present invention provides new methods of delaying senescence in a plant by inhibiting ACC oxidase, or EIN2 activity in the plant. In particular, it is directed to producing green leaves in maize through inhibition of ethylene. The genes involved in producing this phenotype include ACC deaminase, ethylene response sensor (“ERS”), ethylene resistant (“ETR”), and ethylene insensitive (“EIN”). The delay in senescence can be achieved through the production of ACC deaminase, mutated ETR1 and ERS2 proteins, as well as overexpression of wild-type ETR1 and ERS2 proteins.

Abstract: The enzymes of the ACC synthase family are used in producing ethylene. Nucleotide and polypeptide sequences of ACC synthases are provided along with knockout plant cells having inhibition in expression and/or activity in an ACC synthase and knockout plants displaying a staygreen phenotype, a male sterility phenotype, or an inhibition in ethylene production. Methods for modulating staygreen potential in plants, methods for modulating sterility in plants, and methods for inhibiting ethylene production in plants are also provided.

Abstract: The enzymes of the ACC synthase family are used in producing ethylene. Nucleotide and polypeptide sequences of ACC synthases are provided along with knockout plant cells having inhibition in expression and/or activity in an ACC synthase and knockout plants displaying a staygreen phenotype, a male sterility phenotype, or an inhibition in ethylene production. Methods for modulating staygreen potential in plants, methods for modulating sterility in plants, and methods for inhibiting ethylene production in plants are also provided.

Abstract: A gene expression system for controllable expression of ethylene response in a plant cell includes an activation cassette comprising a DNA-binding domain that recognizes a response element; an ecdysone receptor ligand binding domain; and an activation domain; and a target cassette comprising an inducible promoter, which comprises, in operative association, the response element and a minimal promoter responsive to the activation domain. The inducible promoter controls the expression of a nucleic acid sequence that encodes a selected protein that modifies sensitivity to ethylene in the plant. Interaction among the components of the activation cassette and target cassette, when in a plant cell, in the presence of an inducing composition, modulates expression of the selected protein and selectively modulates ethylene sensitivity in the plant cell. This modulation in the expression of the protein is controlled by the timing, the concentration and the duration of the application of the inducing composition.

Abstract: The present invention provides new methods of delaying senescence in a plant by inhibiting ACC oxidase, or EIN2 activity the plant. In particular, it is directed to producing green leaves in maize through inhibition of ethylene. The genes involved in producing this phenotype include ACC deaminase, ethylene response sensor (“ERS”), ethylene resistan (“ETR”), and ethylene insensitive (“EIN”). The delay in senescence can be achieved through the production of ACC deaminase, mutated ETR1 and ERS2 proteins, as well as overexpression of wild-type ETR1 and ERS2 proteins.

Abstract: The present invention is directed to plant genetic engineering. In particular, it is directed to producing green leaves and increasing productivity through inhibition of ethylene. The compositions and methods of the invention involve dominant negative ethylene receptors that interfere with ethylene signaling.

Abstract: The present invention is directed to plant genetic engineering. In particular, it is directed to producing green leaves in maize through inhibition of ethylene. The genes involved in producing this phenotype include 1-Aminocyclopropane-1-Carboxylate (“ACC”) synthase, ACC oxidase, ACC deaminase, ethylene response sensor (“ERS”), ethylene resistant (“ETR”), and ethylene insensitive (“EIN”).