Abstract: An apparatus for the preparation of multiple pollen samples for cryogenic preservation comprising a plurality of chambers for the storage of pollen samples. A vacuum pump reduces the pressure within the interior of the chambers through a vacuum manifold that operatively connects with the pollen samples in the interior of the chambers. An air source vents the interior of the chambers to atmospheric pressure through an air manifold operatively connected with the pollen samples in the interior of the chambers. A vacuum controller controls the pressure within the interior of the chambers through operative connections to the vacuum manifold, the air manifold, the air source, and the vacuum pump. The vacuum controller pulses the pressure with the interior of the chambers between a first and a second reduced pressure level. A sensor operatively connected to the chambers senses the moisture content of the pollen samples within the interior of the chambers at the reduced pressure levels.