Patents by Inventor David A. Routenberg
David A. Routenberg has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240060115Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: November 3, 2023Publication date: February 22, 2024Applicant: Prognosys Biosciences, Inc.Inventors: Mark S. Chee, David A. Routenberg
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Patent number: 11821024Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: March 31, 2023Date of Patent: November 21, 2023Assignee: Prognosys Biosciences, Inc.Inventors: Mark S. Chee, David A. Routenberg
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Publication number: 20230349920Abstract: The invention relates to method and kits for highly specific isolation of surface marker displaying agents from the central nervous system by targeting at least two surface markers. The invention further relates to methods and kits for analyzing surface marker displaying agents and their contents.Type: ApplicationFiled: September 2, 2021Publication date: November 2, 2023Inventors: David ROUTENBERG, Sigal SHACHAR
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Publication number: 20230304074Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: March 31, 2023Publication date: September 28, 2023Applicant: Prognosys Biosciences, Inc.Inventors: Mark S. Chee, David A. Routenberg
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Patent number: 11753674Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: July 31, 2020Date of Patent: September 12, 2023Assignee: Prognosys Biosciences, Inc.Inventors: Mark S. Chee, David A. Routenberg
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Patent number: 11618918Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: June 2, 2022Date of Patent: April 4, 2023Assignee: Prognosys Biosciences, Inc.Inventors: Mark S. Chee, David A. Routenberg
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Patent number: 11525825Abstract: The present invention is directed to kits for improving assay specificity and performance in binding assays.Type: GrantFiled: September 9, 2019Date of Patent: December 13, 2022Assignee: MESO SCALE TECHNOLOGIES, LLC.Inventors: Anahit Aghvanyan, Eli N. Glezer, John Kenten, George Sigal, Martin Stengelin, David Routenberg
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Publication number: 20220357318Abstract: The present invention is directed to methods for improving assay specificity and performance in binding assays.Type: ApplicationFiled: July 21, 2022Publication date: November 10, 2022Inventors: Anahit AGHVANYAN, Eli N. GLEZER, John KENTEN, George SIGAL, Martin STENGELIN, David ROUTENBERG
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Publication number: 20220325325Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: June 2, 2022Publication date: October 13, 2022Applicant: Prognosys Biosciences, Inc.Inventors: Mark S. CHEE, David A. ROUTENBERG
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Publication number: 20220260581Abstract: The present invention provides methods for constructing peptide construct sets and methods of use of these peptide construct sets in assay systems for peptide analysis, and in particular for use in high throughput peptide analysis. The methods allow for analysis of large sets of peptide constructs in a cost-effective manner, employing molecular biological techniques that are both robust and easily parallelized. Thus, the methods allow for the construction of peptide construct sets encompassing, e.g., the human proteome.Type: ApplicationFiled: April 22, 2022Publication date: August 18, 2022Applicant: Prognosys Biosciences, Inc.Inventors: Igor A. KOZLOV, Mark S. CHEE, Petr CAPEK, David A. ROUTENBERG
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Patent number: 11359228Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: February 8, 2022Date of Patent: June 14, 2022Assignee: Prognosys Biosciences, Inc.Inventors: Mark S. Chee, David A. Routenberg
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Patent number: 11340232Abstract: The present invention provides methods for constructing peptide construct sets and methods of use of these peptide construct sets in assay systems for peptide analysis, and in particular for use in high throughput peptide analysis. The methods allow for analysis of large sets of peptide constructs in a cost-effective manner, employing molecular biological techniques that are both robust and easily parallelized. Thus, the methods allow for the construction of peptide construct sets encompassing, e.g., the human proteome.Type: GrantFiled: April 30, 2018Date of Patent: May 24, 2022Assignee: Prognosys Biosciences, Inc.Inventors: Igor A. Kozlov, Mark S. Chee, Petr Capek, David A. Routenberg
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Publication number: 20220154255Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: February 8, 2022Publication date: May 19, 2022Applicant: Prognosys Biosciences, Inc.Inventors: Mark S. CHEE, David A. ROUTENBERG
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Patent number: 11286515Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: June 25, 2021Date of Patent: March 29, 2022Assignee: Prognosys Biosciences, Inc.Inventors: Mark S. Chee, David A. Routenberg
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Publication number: 20220003766Abstract: The invention relates to methods and kits for detecting a virus, e.g., a respiratory virus such as a coronavirus, in a biological sample. The invention also relates to methods and kits for detecting and/or quantifying biomarkers, e.g., antibody biomarkers against a viral antigen; inflammatory and/or tissue damage response biomarkers; and/or extracellular vesicles in response to a viral infection.Type: ApplicationFiled: April 30, 2021Publication date: January 6, 2022Inventors: Jacob N. WOHLSTADTER, George SIGAL, Anu MATHEW, Anastasia AKSYUK, David ROUTENBERG, Martin STENGELIN, John KENTEN, Timothy J. BREAK, Seth B. HARKINS, Michael TSIONSKY
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Publication number: 20210389304Abstract: The invention relates to method and kits for highly specific isolation of extracellular vesicles (EVs) by targeting at least two EV surface markers. The invention further relates to methods and kits for analyzing EVs and their contents.Type: ApplicationFiled: May 17, 2019Publication date: December 16, 2021Inventor: David ROUTENBERG
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Publication number: 20210382043Abstract: The invention relates to method and kits for highly specific isolation of surface marker displaying agents by targeting at least two surface markers. The invention further relates to methods and kits for analyzing surface marker displaying agents and their contents.Type: ApplicationFiled: October 23, 2019Publication date: December 9, 2021Inventors: David ROUTENBERG, Alexander K. TUCKER-SCHWARTZ, Sigal SHACHAR
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Publication number: 20210317510Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: June 25, 2021Publication date: October 14, 2021Applicant: Prognosys Biosciences, Inc.Inventors: Mark S. CHEE, David A. ROUTENBERG
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Patent number: 11046996Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.Type: GrantFiled: February 19, 2021Date of Patent: June 29, 2021Assignee: Prognosys Biosciences, Inc.Inventors: Mark S. Chee, David A. Routenberg
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Publication number: 20210172007Abstract: The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the biological targets comprise proteins, and the methods and assay systems do not depend on imaging techniques for the spatial information of the targets. The present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents; and a decoding scheme providing a readout that is digital in nature.Type: ApplicationFiled: February 19, 2021Publication date: June 10, 2021Applicant: Prognosys Biosciences, Inc.Inventors: Mark S. CHEE, David A. ROUTENBERG