Abstract: Provided are methods and compositions for isolating and detecting rare cells from a biological sample containing other types of cells, particularly including debulking that uses a microfabricated filter for filtering samples. The enriched rare cells can be used in a downstream process such as identification, characterization or growth in culture, or in other ways. Also included is a method of determining tumor aggressiveness or the number or proportion of cancer cells in the enriched sample by detecting telomerase activity, nucleic acid or expression after enrichment of rare cells. Also provided is an efficient, rapid method to specifically remove red and white blood cells from a biological sample containing at least one of the cell types, leading to enrichment of rare target cells including circulating tumor (CTC), stromal, mesenchymal, endothelial, fetal, stem, or non-hematopoietic cells et cetera from a blood sample.

Abstract: The present invention provides methods and compositions for isolating and detecting rare cells from a biological sample containing other types of cells. In particular, the present invention includes a debulking step that uses a microfabricated filters for filtering fluid samples and the enriched rare cells can be used in a downstream process such as identifies, characterizes or even grown in culture or used in other ways. The invention also include a method of determining the aggressiveness of the tumor or of the number or proportion of cancer cells in the enriched sample by detecting the presence or amount of telomerase activity or telomerase nucleic acid or telomerase expression after enrichment of rare cells. This invention further provides an efficient and rapid method to specifically remove red blood cells as well as white blood cells from a biological sample.

Abstract: Provided are methods and compositions for isolating and detecting rare cells from a biological sample containing other types of cells, particularly including debulking that uses a microfabricated filter for filtering samples. The enriched rare cells can be used in a downstream process such as identification, characterization or growth in culture, or in other ways. Also included is a method of determining tumor aggressiveness or the number or proportion of cancer cells in the enriched sample by detecting telomerase activity, nucleic acid or expression after enrichment of rare cells. Also provided is an efficient, rapid method to specifically remove red and white blood cells from a biological sample containing at least one of the cell types, leading to enrichment of rare target cells including circulating tumor (CTC), stromal, mesenchymal, endothelial, fetal, stem, or non-hematopoietic cells et cetera from a blood sample.

Abstract: The present invention provides methods and compositions for isolating and detecting rare cells from a biological sample containing other types of cells. In particular, the present invention includes a debulking step that uses a microfabricated filters for filtering fluid samples and the enriched rare cells can be used in a downstream process such as identifies, characterizes or even grown in culture or used in other ways. The invention also include a method of determining the aggressiveness of the tumor or of the number or proportion of cancer cells in the enriched sample by detecting the presence or amount of telomerase activity or telomerase nucleic acid or telomerase expression after enrichment of rare cells.

Abstract: The present invention provides methods and compositions for isolating and detecting rare cells from a biological sample containing other types of cells. In particular, the present invention includes a debulking step that uses a microfabricated filters for filtering fluid samples and the enriched rare cells can be used in a downstream process such as identifies, characterizes or even grown in culture or used in other ways. The invention also include a method of determining the aggressiveness of the tumor or of the number or proportion of cancer cells in the enriched sample by detecting the presence or amount of telomerase activity or telomerase nucleic acid or telomerase expression after enrichment of rare cells.

Abstract: An apparatus for molding a plastic closure includes a male mold assembly including a forming pin and an outer pin sleeve which cooperate with a female mold for defining a mold cavity. To facilitate mechanical stripping of a molded closure from the forming pin, pressurized air is introduced into the molded closure through at least one air passageway which extends axially between the forming pin and pin sleeve. By outwardly expanding and forming the plastic closure during ejection from the forming pin, forces to which the closure is subjected during ejection are significantly reduced, thereby desirably reducing the amount of cooling of the closure that is required during the molding cycle prior to ejection. Significantly increased operating speeds for the compression molding apparatus can thus be achieved.

Abstract: An apparatus for molding a plastic closure includes a male mold assembly including a forming pin and an outer pin sleeve which cooperate with a female mold for defining a mold cavity. To facilitate mechanical stripping of a molded closure from the forming pin, pressurized air is introduced into the molded closure through at least one air passageway which extends axially between the forming pin and pin sleeve. By outwardly expanding and forming the plastic closure during ejection from the forming pin, forces to which the closure is subjected during ejection are significantly reduced, thereby desirably reducing the amount of cooling of the closure that is required during the molding cycle prior to ejection. Significantly increased operating speeds for the compression molding apparatus can thus be achieved.

Abstract: An apparatus for molding a plastic closure includes a male mold assembly including a forming pin and an outer pin sleeve which cooperate with a female mold for defining a mold cavity. To facilitate mechanical stripping of a molded closure from the forming pin, pressurized air is introduced into the molded closure through at least one air passageway which extends axially between the forming pin and pin sleeve. By outwardly expanding and forming the plastic closure during ejection from the forming pin, forces to which the closure is subjected during ejection are significantly reduced, thereby desirably reducing the amount of cooling of the closure that is required during the molding cycle prior to ejection. Significantly increased operating speeds for the compression molding apparatus can thus be achieved.

Abstract: An apparatus for molding a plastic closure includes a male mold assembly including a forming pin and an outer pin sleeve which cooperate with a female mold for defining a mold cavity. To facilitate mechanical stripping of a molded closure from the forming pin, pressurized air is introduced into the molded closure through at least one air passageway which extends axially between the forming pin and pin sleeve. By outwardly expanding and forming the plastic closure during ejection from the forming pin, forces to which the closure is subjected during ejection are significantly reduced, thereby desirably reducing the amount of cooling of the closure that is required during the molding cycle prior to ejection. Significantly increased operating speeds for the compression molding apparatus can thus be achieved.

Abstract: A closure includes a promotion piece holding container within a cap to be installed onto a container such as a bottle. The promotion piece receiving container includes a top flange captured by a lip of a seal installed within the cap. The promotion piece receiving container includes a frangible band extending circumferentially around a substantial circumference of the container. The container can include a bottom flange arranged for convenience gripping and removing of the container from the cap, and a pull tab attached to the frangible band to assist in removing the frangible band to open the container for removal of the promotion piece.

Abstract: A closure includes a promotion piece holding container within a cap to be installed onto a container such as a bottle. The promotion piece receiving container includes a top flange captured by a lip of a seal installed within the cap. The promotion piece receiving container includes a frangible band extending circumferentially around a substantial circumference of the container. The container can include a bottom flange arranged for convenience gripping and removing of the container from the cap, and a pull tab attached to the frangible band to assist in removing the frangible band to open the container for removal of the promotion piece.

Abstract: An aseptic/sterile fluid connection between two containers is formed by radio frequency sealing together portions of transfer regions of two containers. Each transfer region has a layer of a relatively high melt temperature material such as a rubber or elastomer positioned between two outer container walls made of a flexible thermoplastic, for example polyvinyl chloride. The transfer regions are placed in registry, and the radio frequency welding fuses together portions of adjacent container outer walls and forms a fluid opening therethrough.

Abstract: An ultrasonic imaging agent is produced by a continuous sonication processing of an aqueous solution of heat-denaturable biocompatible protein. The solution is carefully preheated to a temperature of incipient protein denaturation without forming insolubilized protein. A gaseous fluid, preferably air, is added to the solution. In the sonication, the air-containing solution is foamed, increasing the formation and concentration of microbubbles, and the solution is further heated to insolubilize a portion of the protein, thereby encapsulating the microbubbles and forming particulate microspheres.