Patents by Inventor David Gorin
David Gorin has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10053725Abstract: Methods, reagents, compositions, and kits for in situ interaction determination (ISID) via interaction-dependent polymerase chain reaction (ID-PCR) are provided herein. ISID technology is useful for rapidly evaluating potential small molecule-target interactions from mixtures in a single solution. ISID is compatible with unpurified targets in biological samples and can be used to evaluate ligand-binding in DNA-encoded chemical libraries in cell lysates. ISID is also useful to screen ligand interactions of proteins or other molecules in their native state, including their native post-translational modifications and any interactions with accessory proteins and metabolites, in ways that better reflect their relevant biological environment. Because ISID is compatible with crude cell lysates, difficult-to-purify, poorly soluble, intrinsically unstable, and aggregation-prone targets may also be compatible with this method, without requiring truncation or other strategies used to promote heterologous expression.Type: GrantFiled: April 23, 2014Date of Patent: August 21, 2018Assignee: President and Fellows of Harvard CollegeInventors: David R. Liu, Lynn Marie McGregor, David Gorin, Christoph Erich Dumelin
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Patent number: 10011868Abstract: Methods, reagents, compositions, and kits for reactivity-dependent polymerase chain reaction (RD-PCR) and interaction-dependent polymerase chain reaction (ID-PCR) are provided herein. RD-PCR is a technique useful for determining whether a reactive moiety can form a covalent bond to a target reactive moiety, for example, in screening a library of candidate reactive moieties for reactivity with a target reactive moiety, and in identifying an enzyme substrate, for example, in protease substrate profiling. ID-PCR is a technique useful for determining whether a ligand can non-covalently bind to a target molecule, for example, in screening a library of candidate ligands for non-covalent interaction with a target molecule. RD-PCR and ID-PCR are also useful in detecting the presence of an analyte or an environmental condition.Type: GrantFiled: November 3, 2015Date of Patent: July 3, 2018Assignee: President and Fellows of Harvard CollegeInventors: David R. Liu, David Gorin, Adam Kamlet, Lynn Marie McGregor, Christoph Erich Dumelin
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Publication number: 20160186238Abstract: Methods, reagents, compositions, and kits for reactivity-dependent polymerase chain reaction (RD-PCR) and interaction-dependent polymerase chain reaction (ID-PCR) are provided herein. RD-PCR is a technique useful for determining whether a reactive moiety can form a covalent bond to a target reactive moiety, for example, in screening a library of candidate reactive moieties for reactivity with a target reactive moiety, and in identifying an enzyme substrate, for example, in protease substrate profiling. ID-PCR is a technique useful for determining whether a ligand can non-covalently bind to a target molecule, for example, in screening a library of candidate ligands for non-covalent interaction with a target molecule. RD-PCR and ID-PCR are also useful in detecting the presence of an analyte or an environmental condition.Type: ApplicationFiled: November 3, 2015Publication date: June 30, 2016Applicant: President and Fellows of Harvard CollegeInventors: David R. Liu, David Gorin, Adam Kamlet, Lynn Marie McGregor, Christoph Erich Dumelin
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Publication number: 20160083786Abstract: Methods, reagents, compositions, and kits for in situ interaction determination (ISID) via interaction-dependent polymerase chain reaction (ID-PCR) are provided herein. ISID technology is useful for rapidly evaluating potential small molecule-target interactions from mixtures in a single solution. ISID is compatible with unpurified targets in biological samples and can be used to evaluate ligand-binding in DNA-encoded chemical libraries in cell lysates. ISID is also useful to screen ligand interactions of proteins or other molecules in their native state, including their native post-translational modifications and any interactions with accessory proteins and metabolites, in ways that better reflect their relevant biological environment. Because ISID is compatible with crude cell lysates, difficult-to-purify, poorly soluble, intrinsically unstable, and aggregation-prone targets may also be compatible with this method, without requiring truncation or other strategies used to promote heterologous expression.Type: ApplicationFiled: April 23, 2014Publication date: March 24, 2016Applicant: President and Fellows of Harvard CollegeInventors: David R. Liu, Lynn Marie McGregor, David Gorin, Christoph Erich Dumelin
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Patent number: 9175340Abstract: Methods, reagents, compositions, and kits for reactivity-dependent polymerase chain reaction (RD-PCR) and interaction-dependent polymerase chain reaction (ID-PCR) are provided herein. RD-PCR is a technique useful for determining whether a reactive moiety can form a covalent bond to a target reactive moiety, for example, in screening a library of candidate reactive moieties for reactivity with a target reactive moiety, and in identifying an enzyme substrate, for example, in protease substrate profiling. ID-PCR is a technique useful for determining whether a ligand can non-covalently bind to a target molecule, for example, in screening a library of candidate ligands for non-covalent interaction with a target molecule. RD-PCR and ID-PCR are also useful in detecting the presence of an analyte or an environmental condition.Type: GrantFiled: October 13, 2010Date of Patent: November 3, 2015Assignee: President and Fellows of Harvard CollegeInventors: David R. Liu, David Gorin, Adam Kamlet, Lynn M. McGregor, Christoph E. Dumelin
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Publication number: 20130225426Abstract: Methods, reagents, compositions, and kits for reactivity-dependent polymerase chain reaction (RD-PCR) and interaction-dependent polymerase chain reaction (ID-PCR) are provided herein. RD-PCR is a technique useful for determining whether a reactive moiety can form a covalent bond to a target reactive moiety, for example, in screening a library of candidate reactive moieties for reactivity with a target reactive moiety, and in identifying an enzyme substrate, for example, in protease substrate profiling. ID-PCR is a technique useful for determining whether a ligand can non-covalently bind to a target molecule, for example, in screening a library of candidate ligands for non-covalent interaction with a target molecule. RD-PCR and ID-PCR are also useful in detecting the presence of an analyte or an environmental condition.Type: ApplicationFiled: October 13, 2010Publication date: August 29, 2013Applicant: President and Fellows of Harvard CollegeInventors: David R Liu, David Gorin, Adam Kamlet, Lynn M. McGregor, Christoph E. Dumelin
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Publication number: 20060185615Abstract: What is provided is a device and method for gentling equine, other animals, and in some cases a human of which is obtained through the actions of massage, grooming and pole-gentling methodologies and protocols in which it is thoroughly and effectively enabled by an apparatus comprised of three essential hand-tool components: the elongated extension-pole control handle, the cylindrical nodulated massage/gentler tool-head attachment shaft that emits auto-mechanical vibration stimulus, and the cylindrical cone-shaped bristle covered massage/gentler, groomer, exfoliator, tool-head attachment shaft which provides dander exfoliation, body and leg stimulus. Used regularly in succession or independently, animal gentling, animal health and core relationship foundations can be achieved and maintained.Type: ApplicationFiled: February 17, 2006Publication date: August 24, 2006Inventor: David Gorin