Patents by Inventor David H. Gelfand
David H. Gelfand has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10150990Abstract: The present application provides polynucleotides comprising 5?-tails with sequence segments useful for the detection of target nucleic acid sequences, and methods for their use in detecting target nucleic acids. The polynucleotides are used to amplify a subsequence of a target nucleic acid in the presence of one or more ribonucleotides. The ribonucleotides are incorporated into amplification products at regular intervals complementary to the 5?-tail sequence segments. Cleavage of amplification products at the bond immediately 3? to incorporated ribonucleotides produces detectably distinct fragments indicative of the presence or absence of a target nucleic acid.Type: GrantFiled: April 9, 2009Date of Patent: December 11, 2018Assignees: Roche Molecular Systems, Inc., CEA/Institut de Genomique—Centre National de GenotypageInventors: David H. Gelfand, Ivo Glynne Gut, Keith A. Bauer, Florence Mauger
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Patent number: 9206476Abstract: The invention provides a method of determining the nucleotide sequence of a target nucleic acid using a reversibly terminating nucleotide that is modified at the 2? position.Type: GrantFiled: December 20, 2006Date of Patent: December 8, 2015Assignee: ROCHE MOLECULAR SYSTEMS, INCInventors: David H. Gelfand, Amar Gupta
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Patent number: 9102924Abstract: Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. The mutant polymerases overcome the inhibitory effects by an intercalating dye. Therefore, the mutant polymerases are useful in a variety of disclosed methods in combination with an intercalating dye. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.Type: GrantFiled: April 16, 2009Date of Patent: August 11, 2015Assignee: Roche Molecular Systems, Inc.Inventors: Keith A. Bauer, Ellen Fiss, David H. Gelfand, Edward S. Smith, Shawn Suko, Olga Budker, Nancy Schoenbrunner, Susanne Stoffel, Thomas Myers
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Patent number: 8962293Abstract: Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.Type: GrantFiled: October 17, 2007Date of Patent: February 24, 2015Assignee: Roche Molecular Systems, Inc.Inventors: Keith A. Bauer, Ellen Fiss, David H. Gelfand, Edward S. Smith, Shawn Suko, Thomas Myers
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Publication number: 20110294168Abstract: Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. The mutant polymerases overcome the inhibitory effects of a variety of polymerase and reverse transcriptase inhibitors. Therefore, the mutant polymerases are useful in a variety of disclosed methods in the presence of such inhibitors.Type: ApplicationFiled: April 15, 2011Publication date: December 1, 2011Applicant: Roche Molecular Systems, Inc.Inventors: Keith A. Bauer, Ellen Fiss, David H. Gelfand, Edward S. Smith, Shawn Suko, Thomas Myers, Joseph San Filippo, Rachel Shahinian
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Patent number: 7745125Abstract: The present invention provides reaction mixtures that include blocked oligonucleotides comprising 2?-terminator nucleotides. The blocked oligonucleotides are rendered extendible when the 2?-terminator nucleotides are removed from the oligonucleotides, e.g., via pyrophosphorolysis. The reaction mixtures can be used in various nucleic acid polymerization and/or amplification assays, among many other applications. In addition to reaction mixtures, the invention also provides related methods and reaction mixtures.Type: GrantFiled: October 18, 2006Date of Patent: June 29, 2010Assignee: Roche Molecular Systems, Inc.Inventors: David H. Gelfand, Keith A. Bauer, Amar P. Gupta, Veeraiah Bodepudi, John Niemiec
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Publication number: 20090280539Abstract: Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. The mutant polymerases overcome the inhibitory effects by an intercalating dye. Therefore, the mutant polymerases are useful in a variety of disclosed methods in combination with an intercalating dye. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.Type: ApplicationFiled: April 16, 2009Publication date: November 12, 2009Applicant: Roche Molecular Systems, Inc.Inventors: Keith A. Bauer, Ellen Fiss, David H. Gelfand, Edward S. Smith, Shawn Suko, Olga Budker, Nancy Schoenbrunner, Susanne Stoffel
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Publication number: 20090263813Abstract: The present application provides polynucleotides comprising 5?-tails with sequence segments useful for the detection of target nucleic acid sequences, and methods for their use in detecting target nucleic acids. The polynucleotides are used to amplify a subsequence of a target nucleic acid in the presence of one or more ribonucleotides. The ribonucleotides are incorporated into amplification products at regular intervals complementary to the 5?-tail sequence segments. Cleavage of amplification products at the bond immediately 3? to incorporated ribonucleotides produces detectably distinct fragments indicative of the presence or absence of a target nucleic acid.Type: ApplicationFiled: April 9, 2009Publication date: October 22, 2009Applicants: Roche Molecular Systems, Inc., Centre National de GenotypageInventors: DAVID H GELFAND, Ivo Glynne Gut, Keith A. Bauer, Florence Mauger
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Publication number: 20090148891Abstract: Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.Type: ApplicationFiled: October 17, 2007Publication date: June 11, 2009Applicant: Roche Molecular Systems, IncInventors: Keith A. Bauer, Ellen Fiss, David H. Gelfand, Edward S. Smith, Shawn Suko
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Publication number: 20080293071Abstract: The invention provides a method of determining the nucleotide sequence of a target nucleic acid using a reversibly terminating nucleotide that is modified at the 2? position.Type: ApplicationFiled: December 20, 2006Publication date: November 27, 2008Applicant: ROCHE MOLECULAR SYSTEMS, INCInventors: David H. Gelfand, Amar Gupta
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Patent number: 7445900Abstract: A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5? to 3? nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification, assay.Type: GrantFiled: August 11, 2005Date of Patent: November 4, 2008Assignee: Roche Molecular Systems, Inc.Inventors: David H. Gelfand, Pamela M. Holland, Randall K. Saiki, Robert M. Watson
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Publication number: 20080171315Abstract: A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5? to 3? nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification, assay.Type: ApplicationFiled: August 11, 2005Publication date: July 17, 2008Inventors: David H. Gelfand, Pamela M. Holland, Randall K. Saiki, Robert M. Watson
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Patent number: 7148049Abstract: The present invention provides thermostable or thermoactive DNA polymerases with attenuated 3?-5? exonuclease activity, methods for their synthesis, methods for their use, kits comprising the polymerases, nucleic acids encoding the polymerases and cells comprising such a nucleic acid. The DNA polymerases of the invention are useful in many recombinant DNA techniques, such as nucleic acid amplification by the polymerase chain reaction. The DNA polymerases of the invention allow higher fidelity replication and amplification of a template DNA sequence, allow less degradation of primers and/or more efficient use of deoxynucleotide triphosphates and are in general more efficient and less costly to make and use.Type: GrantFiled: March 26, 2003Date of Patent: December 12, 2006Assignee: Roche Molecular Systems, Inc.Inventors: Nancy J. Schoenbrunner, Thomas W. Myers, David H. Gelfand
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Patent number: 7141377Abstract: A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5? to 3? nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.Type: GrantFiled: January 27, 2005Date of Patent: November 28, 2006Assignee: Roche Molecular Systems, Inc.Inventors: David H. Gelfand, Pamela M. Holland, Randall K. Saiki, Robert M. Watson
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Publication number: 20040005599Abstract: The present invention provides thermostable or thermoactive DNA polymerases with attenuated 3′-5′ exonuclease activity, methods for their synthesis, methods for their use, kits comprising the polymerases, nucleic acids encoding the polymerases and cells comprising such a nucleic acid. The DNA polymerases of the invention are useful in many recombinant DNA techniques, such as nucleic acid amplification by the polymerase chain reaction. The DNA polymerases of the invention allow higher fidelity replication and amplification of a template DNA sequence, allow less degradation of primers and/or more efficient use of deoxynucleotide triphosphates and are in general more efficient and less costly to make and use.Type: ApplicationFiled: March 26, 2003Publication date: January 8, 2004Inventors: Nancy J. Schoenbrunner, Thomas W. Myers, David H. Gelfand
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Patent number: 6514736Abstract: The present invention is directed to a process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof using a thermostable enzyme. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.Type: GrantFiled: November 1, 2000Date of Patent: February 4, 2003Assignee: Roche Molecular Systems, IncInventors: Henry A. Erlich, Glenn Horn, Randall K. Saiki, Kary B. Mullis, David H. Gelfand
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Patent number: 6214979Abstract: A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.Type: GrantFiled: September 19, 1997Date of Patent: April 10, 2001Assignee: Roche Molecular SystemsInventors: David H. Gelfand, Pamela M. Holland, Randall K. Saiki, Robert M. Watson
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Patent number: 6197563Abstract: The present invention is directed to a process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof using a thermostable enzyme. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.Type: GrantFiled: November 18, 1994Date of Patent: March 6, 2001Assignee: Roche Molecular Systems, Inc.Inventors: Henry A. Erlich, Glenn Horn, Randall K. Saiki, Kary B. Mullis, David H. Gelfand
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Patent number: 6127155Abstract: A purified thermostable nucleic acid polymerase is obtained that has unique characteristics. Preferably the nucleic acid polymerase is DNA polymerase isolated from a Thermus aquaticus species and has a molecular weight of about 86,000-95,000 daltons. The thermostable nucleic acid polymerase may be native or recombinant and may be used in a temperature-cycling chain reaction wherein at least one nucleic acid sequence is amplified in quantity from an existing sequence with the aid of selected primers and nucleotide triphosphates. The nucleic acid polymerase is preferably stored in a buffer containing non-ionic detergents that lends stability to the nucleic acid polymerase. A preferred buffer contains glycerol, polyoxyethylated sorbitan monolaurate, ethoxylated nonyl phenol and gelatin.Type: GrantFiled: April 24, 1992Date of Patent: October 3, 2000Assignee: Roche Molecular Systems, Inc.Inventors: David H. Gelfand, Susanne Stoffel, Randall K. Saiki
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Patent number: 6040166Abstract: The present invention is directed to a process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof using a thermostable enzyme. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.Type: GrantFiled: September 27, 1994Date of Patent: March 21, 2000Assignee: Roche Molecular Systems, Inc.Inventors: Henry A. Erlich, Glenn Horn, Randall K. Saiki, Kary B. Mullis, David H. Gelfand