Abstract: First and second sets of receptacles containing reagents for first and second amplification reactions, respectively, are placed in first and second receptacle holders, each associated with a thermal element. The receptacles are subjected to different first and second incubation processes resulting in the first and second amplification reactions in each of the first and second sets of receptacles that contain a first or a second target nucleic acid, respectively. The presence or absence of the first or second target nucleic acid, if any, is determined for each receptacle of the first and second sets of receptacles.

Abstract: System, apparatus, and method for cycling the temperature of at least one receptacle holder that is adapted for use in an automated instrument capable of performing nucleic acid-based amplification tests. Also provided are methods for conducting automated, random-access incubation processes using the same.

Abstract: A thermally conductive receptacle holder includes a plurality of wells that each receive a receptacle. A cross-brace is mounted to a support to exert a force onto an outer surface of a compressive housing such that the compressive housing exerts a force onto the receptacle holder. An optical fiber associated with each well provides optical communication between the receptacle well and an excitation signal source and/or an emission signal detector.

Abstract: A receptacle transport mechanism comprises a body, a plunger configured to be movable with respect to the body, and one or more limbs movably attached to the body and positioned in operative engagement with the plunger. The plunger is movable with respect to the body between a first position whereby engagement by the plunger with the one or more limbs positions a lower portion of each limb proximal to the body and a second position whereby engagement by the plunger with the one or more limbs extends the lower portion of each limb outwardly relative to the body.

Abstract: First and second sets of receptacles containing reagents for first and second amplification reactions, respectively, are placed in first and second receptacle holders, each associated with a thermal element. The receptacles are subjected to different first and second incubation processes resulting in the first and second amplification reactions in each of the first and second sets of receptacles that contain a first or a second target nucleic acid, respectively. The presence or absence of the first or second target nucleic acid, if any, is determined for each receptacle of the first and second sets of receptacles.

Abstract: System, apparatus, and method for cycling the temperature of at least one receptacle holder that is adapted for use in an automated instrument capable of performing nucleic acid-based amplification tests. Also provided are methods for conducting automated, random-access incubation processes using the same.