Abstract: An aseptic cell culture system including a cell culture device is disclosed. The cell culture device includes an interior chamber having a first opening extending through a wall defining a part of the interior chamber, and a first aseptic port. The first aseptic port includes a first coupling end and a second coupling end, the first coupling end extending through the first opening and integrated with the wall. Further, the first aseptic port includes a fluid pathway in fluid communication with the interior chamber and extends between the first coupling end and the second coupling end. The second coupling end includes a connecting component configured to allow one or more of a fluid line and a filter to be connected to the first aseptic port.