Patents by Inventor David Kovalic

David Kovalic has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20070089195
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: October 10, 2006
    Publication date: April 19, 2007
    Inventors: Shihshieh Huang, Thomas Malvar, Michael Luethy, Philip Miller, Larry Gilbertson, Edwards Allen, Sara Heisel, David Kovalic, James Roberts, Nancy Houmard, Alessandra Frizzi, Yuanji Zhang, Liang Guo, Linda Lutfiyya
  • Publication number: 20070089194
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: October 10, 2006
    Publication date: April 19, 2007
    Inventors: Shihshieh Huang, Thomas Malvar, Michael Luethy, Philip Miller, Larry Gilbertson, Edwards Allen, Sara Heisel, David Kovalic, James Roberts, Nancy Houmard, Alessandra Frizzi, Yuanji Zhang, Liang Guo, Linda Lutfiyya
  • Publication number: 20070089187
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: October 10, 2006
    Publication date: April 19, 2007
    Inventors: Shihshieh Huang, Thomas Malvar, Michael Luethy, Philip Miller, Larry Gilbertson, Edwards Allen, Sara Heisel, David Kovalic, James Roberts, Nancy Houmard, Alessandra Frizzi, Yuanji Zhang, Liang Guo, Linda Lutfiyya
  • Publication number: 20070089190
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: October 10, 2006
    Publication date: April 19, 2007
    Inventors: Shihshieh Huang, Thomas Malvar, Michael Luethy, Philip Miller, Larry Gilbertson, Edwards Allen, Sara Heisel, David Kovalic, James Roberts, Nancy Houmard, Alessandra Frizzi, Yuanji Zhang, Liang Guo, Linda Lutfiyya
  • Publication number: 20070089193
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: October 10, 2006
    Publication date: April 19, 2007
    Inventors: Shihshieh Huang, Thomas Malvar, Michael Luethy, Philip Miller, Larry Gilbertson, Edwards Allen, Sara Heisel, David Kovalic, James Roberts, Nancy Houmard, Alessandra Frizzi, Yuanji Zhang, Liang Guo, Linda Lutfiyya
  • Publication number: 20070089191
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: October 10, 2006
    Publication date: April 19, 2007
    Inventors: Shihshieh Huang, Thomas Malvar, Michael Luethy, Philip Miller, Larry Gilbetson, Edwards Allen, Sara Heisel, David Kovalic, James Roberts, Nancy Houmard, Alessandra Frizzi, Yuanji Zhang, Liang Guo, Linda Lutfiyya
  • Publication number: 20070089196
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: October 10, 2006
    Publication date: April 19, 2007
    Inventors: Shihshieh Huang, Thomas Malvar, Michael Luethy, Philip Miller, Larry Gilbertson, Edwards Allen, Sara Heisel, David Kovalic, James Roberts, Nancy Houmard, Alessandra Frizzi, Yuanji Zhang, Liang Guo, Linda Lutfiyya
  • Publication number: 20070089184
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: October 10, 2006
    Publication date: April 19, 2007
    Inventors: Shihshieh Huang, Thomas Malvar, Michael Luethy, Philip Miller, Larry Gilbertson, Edwards Allen, Sara Heisel, David Kovalic, James Roberts, Nancy Houmard, Alessandra Frizzi, Yuanji Zhang, Liang Guo, Linda Lutfiyya
  • Publication number: 20070089188
    Abstract: The present invention provides molecular consrtucts and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: October 10, 2006
    Publication date: April 19, 2007
    Inventors: Shihshieh Huang, Thomas Malvar, Michael Luethy, Philip Miller, Larry Gilbertson, Edwards Allen, Sara Heisel, David Kovalic, James Roberts, Nancy Houmard, Alessandra Frizzi, Yuanji Zhang, Liang Guo, Linda Lutfiyya
  • Publication number: 20070089192
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: October 10, 2006
    Publication date: April 19, 2007
    Inventors: Shihshieh Huang, Thomas Malvar, Michael Luethy, Philip Miller, Larry Gilbertson, Edwards Allen, Sara Heisel, David Kovalic, James Roberts, Nancy Houmard, Alessandra Frizzi, Yuanji Zhang, Liang Guo, Linda Lutfiyya
  • Publication number: 20070089185
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: October 10, 2006
    Publication date: April 19, 2007
    Inventors: Shihshieh Huang, Thomas Malvar, Michael Luethy, Philip Miller, Larry Gilbertson, Edwards Allen, Sara Heisel, David Kovalic, James Roberts, Nancy Houmard, Alessandra Frizzi, Yuanji Zhang, Liang Guo, Linda Lutfiyya
  • Publication number: 20070089186
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: October 10, 2006
    Publication date: April 19, 2007
    Inventors: Shihshieh Huang, Thomas Malvar, Michael Luethy, Philip Miller, Larry Gilbertson, Edwards Allen, Sara Heisel, David Kovalic, James Roberts, Nancy Houmard, Alessandra Frizzi, Yuanji Zhang, Liang Guo, Linda Lutfiyya
  • Publication number: 20070083951
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: October 10, 2006
    Publication date: April 12, 2007
    Inventors: Shihshieh Huang, Thomas Malvar, Michael Luethy, Philip Miller, Larry Gilbertson, Edwards Allen, Sara Heisel, David Kovalic, James Roberts, Nancy Houmard, Alessandra Frizzi, Yuanji Zhang, Liang Guo, Linda Lutfiyya
  • Publication number: 20070083949
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: October 10, 2006
    Publication date: April 12, 2007
    Inventors: Shihshieh Huang, Thomas Malvar, Michael Luethy, Philip Miller, Larry Gilbertson, Edwards Allen, Sara Heisel, David Kovalic, James Roberts, Nancy Houmard, Alessandra Frizzi, Yuanji Zhang, Liang Guo, Linda Lutfiyya
  • Publication number: 20070083947
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: October 10, 2006
    Publication date: April 12, 2007
    Inventors: Shihshieh Huang, Thomas Malvar, Michael Luethy, Philip Miller, Larry Gilbertson, Edwards Allen, Sara Heisel, David Kovalic, James Roberts, Nancy Houmard, Alessandra Frizz, Yuanji Zhang, Liang Guo, Linda Lutfiyya
  • Publication number: 20070083945
    Abstract: The present invention is in the field of plant genetics. More specifically the invention relates to nucleic acid molecules and nucleic acid molecules that contain markers, in particular, single nucleotide polymorphism (SNP) and repetitive element markers. In addition, the present invention provides nucleic acid molecules having regulatory elements or encoding proteins or fragments thereof. The invention also relates to proteins and fragments of proteins so encoded and antibodies capable of binding the proteins. The invention also relates to methods of using the nucleic acid molecules, markers, repetitive elements and fragments of repetitive elements, regulatory elements, proteins and fragments of proteins.
    Type: Application
    Filed: August 2, 2006
    Publication date: April 12, 2007
    Inventors: Joseph Byrum, Conrad Halling, David Kovalic
  • Publication number: 20070083952
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: October 10, 2006
    Publication date: April 12, 2007
    Inventors: Shihshieh Huang, Thomas Malvar, Michael Luethy, Philip Miller, Larry Gilbertson, Edwards Allen, Sara Heisel, David Kovalic, James Roberts, Nancy Houmard, Alessandra Frizz, Yuanji Zhang, Liang Guo, Linda Lutfiyya
  • Publication number: 20070083950
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: October 10, 2006
    Publication date: April 12, 2007
    Inventors: Shihshieh Huang, Thomas Malvar, Michael Luethy, Philip Miller, Larry Gilbertson, Edwards Allen, Sara Heisel, David Kovalic, James Roberts, Nancy Houmard, Alessandra Frizzi, Yuanji Zhang, Liang Guo, Linda Lutfiyya
  • Publication number: 20070067865
    Abstract: The present invention is in the field of plant biochemistry. More specifically the invention relates to nucleic acid sequences from plant cells, in particular, nucleic acid sequences from maize, teosinte, soybean, Arabidopsis, cotton, Sorghum, rice and wheat. The invention encompasses nucleic acid molecules that encode proteins and fragments of proteins. In addition, the invention also encompasses proteins and fragments of proteins so encoded and antibodies capable of binding these proteins or fragments. The invention also relates to methods of using the nucleic acid molecules, proteins and fragments of proteins, and antibodies, for example for genome mapping, gene identification and analysis, plant breeding, preparation of constructs for use in plant gene expression, and transgenic plants.
    Type: Application
    Filed: August 14, 2006
    Publication date: March 22, 2007
    Inventors: David Kovalic, Jingdong Liu
  • Publication number: 20070061916
    Abstract: Recombinant polynucleotides and recombinant polypeptides useful for improvement of plants are provided. The disclosed recombinant polynucleotides and recombinant polypeptides find use in production of transgenic plants to produce plants having improved properties.
    Type: Application
    Filed: July 14, 2006
    Publication date: March 15, 2007
    Inventors: David Kovalic, Yihua Zhou, Yongwei Cao