Abstract: The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.

Abstract: The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.

Abstract: Apparatus, methods, and systems for determining point locations with respect to an origin point. Aspects provide for a cost-effective and easy to use way in which a limited number of persons may map out point locations over a relatively large area with a high degree of accuracy.

Abstract: Methods and apparatuses are provided that can be utilized for accurate pre-aiming and installation of devices. The devices are pre-set to an aiming orientation relative to a universal reference plane. The reference plane is then correlated to a feature of a pole, tower, or other structure that will be used to elevate or suspend the devices. A position sensing subsystem is utilized to inform a worker when each device is correctly angularly oriented to the reference plane. The worker simply moves the mounting structure for the device to the correct three-dimensional angular orientation, uses the position sensor to confirm the correct orientation to within a highly accurate margin of error, and either locks the device in that orientation or marks the orientation. The pole, tower, or other elevating structure is then preliminarily erected at its pre-designed location and pre-designed rotational orientation with the pre-aimed devices.

Abstract: Disclosed herein are apparatus, methods, and systems for grounding outdoor light poles, as well as other structures, which may be exposed to lightning or other adverse electrical effects and may require a low impedance path to ground. Inventive aspects include a combination of apparatus integral to the pole or other structure and installation considerations whereby the ease of installation, reduction of onsite installation error, and reduction of impedance may be tailored to each installation. An apparatus can include a pre-installed earth grounding electrode at the lower end of the pole or structure to be inserted into the earth. A method can include installing an earth grounding electrode to/on/in a lower end of a pole or structure prior to insertion into the earth.

Abstract: Disclosed herein are apparatus, methods, and systems for grounding outdoor light poles, as well as other structures, which may be exposed to lightning or other adverse electrical effects and may require a low impedance path to ground. Inventive aspects include a combination of apparatus integral to the pole or other structure and installation considerations whereby the ease of installation, reduction of onsite installation error, and reduction of impedance may be tailored to each installation. An apparatus can include a pre-installed earth grounding electrode at the lower end of the pole or structure to be inserted into the earth. A method can include installing an earth grounding electrode to/on/in a lower end of a pole or structure prior to insertion into the earth.

Abstract: A method of laying out a grid of points across an area comprises designating a starting reference point; projecting with the laser beam a first reference line intersecting the first reference point and extending in a first direction relative to the area; designating the set of first line reference points along the first reference line; projecting with a laser beam a second reference line from each of the set of first reference line points in a direction at least partially across the area; designating a set of second line reference points at or near the area along each of the second reference lines, so that a grid of points across the area correlated to at least one laser beam projection is created. In this manner, the laser provides a quick and accurate straight line for referencing points in the grid. The method could be used to just lay out a grid of lines.

Abstract: A method of laying out a grid of points across an area comprises designating a starting reference point; projecting with the laser beam a first reference line intersecting the first reference point and extending in a first direction relative to the area; designating the set of first line reference points along the first reference line; projecting with a laser beam a second reference line from each of the set of first reference line points in a direction at least partially across the area; designating a set of second line reference points at or near the area along each of the second reference lines, so that a grid of points across the area correlated to at least one laser beam projection is created. In this manner, the laser provides a quick and accurate straight line for referencing points in the grid. The method could be used to just lay out a grid of lines.

Abstract: Disclosed herein are apparatus, methods, and systems for determining point locations with respect to an origin point. Aspects described herein provide for a cost-effective and easy to use way in which a limited number of persons may map out point locations over a relatively large area with a high degree of accuracy.

Abstract: A method of laying out a grid of points across an area comprises designating a starting reference point; projecting with the laser beam a first reference line intersecting the first reference point and extending in a first direction relative to the area; designating the set of first line reference points along the first reference line; projecting with a laser beam a second reference line from each of the set of first reference line points in a direction at least partially across the area; designating a set of second line reference points at or near the area along each of the second reference lines, so that a grid of points across the area correlated to at least one laser beam projection is created. In this manner, the laser provides a quick and accurate straight line for referencing points in the grid. The method could be used to just lay out a grid of lines.

Abstract: Disclosed herein are apparatus, methods, and systems for grounding outdoor light poles, as well as other structures, which may be exposed to lightning or other adverse electrical effects and may require a low impedance path to ground. Inventive aspects include a combination of apparatus integral to the pole or other structure and installation considerations whereby the ease of installation, reduction of onsite installation error, and reduction of impedance may be tailored to each installation. An apparatus can include a pre-installed earth grounding electrode at the lower end of the pole or structure to be inserted into the earth. A method can include installing an earth grounding electrode to/on/in a lower end of a pole or structure prior to insertion into the earth.

Abstract: The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.

Abstract: A method of laying out a grid of points across an area comprises designating a starting reference point; projecting with the laser beam a first reference line intersecting the first reference point and extending in a first direction relative to the area; designating the set of first line reference points along the first reference line; projecting with a laser beam a second reference line from each of the set of first reference line points in a direction at least partially across the area; designating a set of second line reference points at or near the area along each of the second reference lines, so that a grid of points across the area correlated to at least one laser beam projection is created. In this manner, the laser provides a quick and accurate straight line for referencing points in the grid. The method could be used to just lay out a grid of lines.

Abstract: An apparatus and method of evaluating lighting for a given target area that will have an event which is televised. The method includes calculating television foot-candle and vertical foot-candle for various points on the target area as well as calculating the ratio between those two readings. These calculated readings can then be evaluated for the purpose of determining whether sufficient uniformity and intensity exists for given aiming locations on the target area to provide high quality television filming. The apparatus is preferably a computer program that is installed on a computer that would allow both simulations of not yet existing lighting systems to design lighting systems for optimum television coverage or evaluate existing lighting systems to change them for better performance for televising.

Abstract: A capillary electrophoresis separation matrix for single-stranded nucleic acids, along with methods for using and preparing the matrix, are disclosed. The separation matrix provides denaturing conditions and contains hydroxyethyl cellulose (HEC) in combination with urea, and preferably also includes formamide. The separation matrix may be used for DNA sizing and sequencing applications and provides a single-base resolution to approximately 500 base pairs. The separation matrix is inexpensive, easy to prepare, requires no polymerization steps, and is of low enough viscosity to be pumped easily into and out of capillary tubes for electrophoresis. The low viscosity allows for high throughput of samples and reuse of the capillary tubes for numerous separations.

Abstract: A capillary electrophoresis separation matrix for single-stranded nucleic acids, along with methods for using and preparing the matrix, are disclosed. The separation matrix provides denaturing conditions and contains hydroxyethyl cellulose (HEC) in combination with urea, and preferably also includes formamide. The separation matrix may be used for DNA sizing and sequencing applications and provides a single-base resolution to approximately 500 base pairs. The separation matrix is inexpensive, easy to prepare, requires no polymerization steps, and is of low enough viscosity to be pumped easily into and out of capillary tubes for electrophoresis. The low viscosity allows for high throughput of samples and reuse of the capillary tubes for numerous separations.

Abstract: Using storage phosphor recording media, two exposures are made of a sample containing two target substances tagged with different radiative emitters, for example 32-P and 35-S. The first exposure, image 1, is of the sample directly on the phosphor screen. The second exposure, image 2, is made with the appropriate absorption material between the sample and screen. Both images are captured. By using simultaneous equations isotope contributions of each label can be determined. Quantitative assessment of the contributions of the isotopes, and hence the target substances, are made by calculating isotope efficiencies both with and without the absorption filter.

Abstract: Toy figurines including toy dolls are disclosed that have skins or exterior surfaces repeatedly capable of reversibly changing color. The skin is coated with a sealant/primer base coat of flexible, hydrophobic film-forming polymeric material. Applied over the base coat is a color layer which is either continuous or of desired geometric configuration. The color layer contains acid-base indicating material, preferably microencapsulated, matrixed in a mixture of polymeric material. A top coat applied over the color layer protects the color layer against abrasion and is water permeable. The coated skin portions thus change color in response to changes in the pH of aqueous solutions applied to them.