Abstract: Provided herein are methods of identifying genomic region(s) predictive of an outcome of treatment with a cell therapy and/or of a phenotype of function of the cells. In some embodiments, the methods include epigenetic and/or epigenomic analyses of the cells in connection with methods for preparing engineered cells for cell therapy and/or predicting response to a cell therapy, e.g., engineered cells for cell therapy. In some embodiments, the methods include steps to assess, characterize and analyze changes or modifications in an epigenetic property of gene region or regions, such as chromatin accessibility, nucleosome occupancy, histone modification, spatial chromosomal conformation, transcription factor occupancy and/or DNA methylation. In some embodiments, the epigenetic and/or epigenomic analysis includes determining the epigenetic properties of a cell, e.g., an engineered cell for cell therapy.

Abstract: A fuel pressure regulator comprising a housing, a diaphragm dividing the housing into a first chamber and second chamber, a passage from the exterior of the housing to the first chamber, an inlet and an outlet associated with the second chamber of the housing, and a valve seat associated with the outlet. A cage is mounted on and movable with the diaphragm and a spring within the first chamber yieldingly urges the cage toward the valve seat. The cage supports a pair of plates which retain a valve ball. The first plate has an opening through which a portion of the ball projects and a second plate retains the first plate and the ball within the cage. The first plate is movable transversely to provide for proper centering of the ball in the valve seat. A light spring extends between the cage and the ball to urge the ball against the first plate.