Abstract: Disclosed herein are methods and compositions comprising a polymerase and a phosphorylated nucleoside, wherein the polymerase and the nucleoside are covalently linked by a cleavable linker at the terminal phosphate group. Further disclosed herein are enzymatic polynucleotide synthesis using polymerase and nucleotide conjugation strategies.

Abstract: Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleotide connected to a nucleic acid comprising a FRET label comprising at least three luminescent molecules. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.

Abstract: Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleic acid connected to a nucleotide and two or more luminescent labels. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.

Abstract: A system including a method and device for real-time synchronization of MIDI generated sounds with audio program material originating from a Digital Audio Workstation (DAW). Provided is a real-time feed-forward control system for the generation of sound arising upon the execution of MIDI commands time-synchronized with the playback of audio program material. The system uses one or more audio synchronization tracks to accomplish synchronization. An audio interface device converts the data streams created by the playback of audio program tracks and audio synchronization tracks into electrical audio signals. The proposed MIDI interface device uses the audio synchronization signals output by the audio interface device to strobe or clock out MIDI events waiting on a FIFO buffer inside the device. Finally, the re-synchronized MIDI events are transmitted to the sound generating device in perfect synchronization with the audio program material.

Abstract: A compressor system includes an oil reservoir capable of producing a mixed air/oil flow of fluid via a breather port, as well as a cyclonic separator having an inlet annulus and a tapered section extending from the inlet annulus. The inlet annulus structured to swirl the mixed air/oil flow prior to moving toward a tapered internal section of the separator. A center post is positioned in the tapered internal section and includes a number of discs arranged along a length of the center post. The discs can be separate discs that are stacked upon one another to form the center post. The discs can include a skirt portion and a neck having one or more openings that permit a flow of at least air to enter into a central bore of the center post from the tapered internal section before exiting the separator.

Abstract: A compressor system includes an oil reservoir capable of producing a mixed air/oil flow of fluid via a breather port, as well as a cyclonic separator having an inlet annulus and a tapered section extending from the inlet annulus. The inlet annulus structured to swirl the mixed air/oil flow prior to moving toward a tapered internal section of the separator. A center post is positioned in the tapered internal section and includes a number of discs arranged along a length of the center post. The discs can be separate discs that are stacked upon one another to form the center post. The discs can include a skirt portion and a neck having one or more openings that permit a flow of at least air to enter into a central bore of the center post from the tapered internal section before exiting the separator.

Abstract: A breast cup for a brassiere, a brassiere, and a method of making the breast cup. The breast cup includes a molded body having an inner surface, an outer surface and an apex. A layer of flock is adhered to the molded body at a location corresponding with the apex in order to provide increased modesty for the breast cup.

Abstract: A breast cup for a brassiere, a brassiere, and a method of making the breast cup. The breast cup includes a molded body having an inner surface, an outer surface and an apex. A layer of flock is adhered to the molded body at a location corresponding with the apex in order to provide increased modesty for the breast cup.

Abstract: A crystal growth apparatus is disclosed comprising a crucible, optionally contained within a crucible box, on a crucible support block, wherein the bottom of the crucible, the bottom plate of the crucible box, if used, and/or the crucible support block comprise at least one cavity configured to circulate at least one coolant therein. Also disclosed is a method of preparing a crystalline material using the disclosed crystal growth apparatus as well as the resulting crystalline material, having larger overall grain sizes.

Abstract: An apparatus and method for underwriting and/or rating an insurance policy, and for generating information for underwriting and/or rating an insurance policy, are provided. The system and method may include identifying a vehicle, determining a value of at least one vehicle history data variable or a group of variables, and underwriting and/or rating the policy based on the value of the at least one vehicle history data variable or a group of variables. The system and method may also include generating a score based on the value of the at least one vehicle history data variable or group of variables.

Abstract: An apparatus and method for underwriting and/or rating an insurance policy, and for generating information for underwriting and/or rating an insurance policy, are provided. The system and method may include identifying a vehicle, determining a value of at least one vehicle history data variable or a group of variables, and underwriting and/or rating the policy based on the value of the at least one vehicle history data variable or a group of variables. The system and method may also include generating a score based on the value of the at least one vehicle history data variable or group of variables.

Abstract: An apparatus and method for underwriting and/or rating an insurance policy, and for generating information for underwriting and/or rating an insurance policy, are provided. The system and method may include identifying a vehicle, determining a value of at least one vehicle history data variable or a group of variables, and underwriting and/or rating the policy based on the value of the at least one vehicle history data variable or a group of variables. The system and method may also include generating a score based on the value of the at least one vehicle history data variable or group of variables.

Abstract: An apparatus and method for underwriting and/or rating an insurance policy, and for generating information for underwriting and/or rating an insurance policy, are provided. The system and method may include identifying a vehicle, determining a value of at least one vehicle history data variable or a group of variables, and underwriting and/or rating the policy based on the value of the at least one vehicle history data variable or a group of variables. The system and method may also include generating a score based on the value of the at least one vehicle history data variable or group of variables.

Abstract: A system and method of identifying warning signs of fraud associated with vehicle insurance transactions is presented. The system and method operate by identifying a vehicle, retrieving vehicle history information about the vehicle and determining if the vehicle history information indicates potential fraud based on factors which show a relationship between said vehicle history information and potential fraud incidents. Inferences related to an insurance claim or quote are made based on the results of the determination.

Abstract: A design structure for monitoring of the performance of semiconductor circuits, such as circuit delay, across a chip. The design structure may include a clock source and a plurality of process monitors. The design structure may be used to construct a “schmoo plot” by varying a frequency of the clock source to determine the delay of process monitors at various locations across the chip.

Abstract: An apparatus and method for underwriting and/or rating an insurance policy, and for generating information for underwriting and/or rating an insurance policy, are provided. The system and method may include identifying a vehicle, determining a value of at least one vehicle history data variable or a group of variables, and underwriting and/or rating the policy based on the value of the at least one vehicle history data variable or a group of variables. The system and method may also include generating a score based on the value of the at least one vehicle history data variable or group of variables.

Abstract: An edge triggered system is provided having a data and scan input includes a latch device having a clock input and an AND gate, coupled to the latch device, structured and arranged to receive a first clock signal and an inverted clock signal to generate a clock to the clock input. A process for operating an edge triggered system having a data and scan input includes forwarding a first clock signal to an input of an AND gate. The method includes inverting a second clock signal forwarded to another input of the AND gate and generating a clock input for a latch device from the AND gate.

Abstract: A method of synchronous digital operation and scan based testing of an integrated circuit using a flip-flop. The flip-flop including a master latch having an input and a clock pin; a slave latch having an output, a first clock pin and a second clock pin, the slave latch connected to the master latch; a first AND gate having a first input, an inverted second input and an output, the output of the first AND gate connected to the first clock pin of the master latch; a second AND gate having a first input, an inverted second input and an output, the output of the second AND gate connected to the second input of the first AND gate and to the first clock pin of the slave latch.

Abstract: Built-in self-test (BIST) microcontroller integrated circuit adapted for logic verification. Microcontroller includes a plurality of hardware description language files representing a hierarchical description of the microcontroller, the plurality of hardware description language files including a library of circuit design elements, a plurality of library design circuit elements adapted to store a uniquely defined set of input and output signals to enable a logic BIST, and a plurality of latches adapted to store a plurality of values corresponding to a behavioral profile of a test clock.