Patents by Inventor David M. Lough
David M. Lough has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20090023150Abstract: Fast and highly accurate mass spectrometry-based processes for detecting a particular nucleic acid sequence in a biological sample are provided. Depending on the sequence to be detected, the processes can be used, for example, to diagnose a genetic disease or chromosomal abnormality; a predisposition to a disease or condition, infection by a pathogenic organism, or for determining identity or heredity.Type: ApplicationFiled: June 27, 2008Publication date: January 22, 2009Applicant: SEQUENOM, INC.Inventors: Hubert Koster, Daniel P. Little, Andreas Braun, David M. Lough, Guobing Xiang, Dirk van den Boom, Christian Jurinke, Andreas Ruppert
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Patent number: 6812455Abstract: Matrix assisted laser desorption/ionization (MALDI) is performed in a manner that thermalizes large analyte ions in a plume of desorbed material for spectroscopic analysis. The thermalized ions have a low or zero mean velocity and are presented at a well-defined instant in time, reducing artifacts and sharpening the spectral peaks. In one embodiment the light is delivered to a matrix or sample holder having a cover, baffle or compartment. The baffle or compartment impedes or contains a plume of desorbed material and the analyte undergoes collisions to lower its mean velocity and directionality. Thus “thermalized” the analyte ions are passed to a mass analysis instrument.Type: GrantFiled: April 3, 2002Date of Patent: November 2, 2004Assignee: Sequenom, Inc.Inventors: Franz Hillenkamp, David M. Lough, G. Scott Higgins, Dirk Reuter
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Publication number: 20030129589Abstract: Fast and highly accurate mass spectrometry-based processes for detecting a particular nucleic acid sequence in a biological sample are provided. Depending on the sequence to be detected, the processes can be used, for example, to diagnose a genetic disease or chromosomal abnormality; a predisposition to a disease or condition, infection by a pathogenic organism, or for determining identity or heredity.Type: ApplicationFiled: June 28, 1999Publication date: July 10, 2003Inventors: HUBERT KOSTER, DAVID M. LOUGH, GOUBING XIANG
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Publication number: 20020109085Abstract: Matrix assisted laser desorption/ionization (MALDI) is performed in a manner that thermalizes large analyte ions in a plume of desorbed material for spectroscopic analysis. The thermalized ions have a low or zero mean velocity and are presented at a well-defined instant in time, reducing artifacts and sharpening the spectral peaks. In one embodiment the light is delivered to a matrix or sample holder having a cover, baffle or compartment. The baffle or compartment impedes or contains a plume of desorbed material and the analyte undergoes collisions to lower its mean velocity and directionality. Thus “thermalized” the analyte ions are passed to a mass analysis instrument.Type: ApplicationFiled: April 3, 2002Publication date: August 15, 2002Inventors: Franz Hillenkamp, David M. Lough, G. Scott Higgins, Dirk Reuter
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Patent number: 6423966Abstract: Matrix assisted laser desorption/ionization is performed,in a manner to thermalize large analyte ions in a plume of desorbed material for spectroscopic analysis. The thermalized ions have a low or zero mean velocity and are presented at a well-defined instant in time, reducing artifacts and sharpening the spectral peaks. In one embodiment the light is delivered to a matrix or sample holder having a cover, baffle or compartment. The baffle or compartment impedes or contains a plume of desorbed material and the analyte undergoes collisions to lower its mean velocity and directionality. Thus “thermalized” the analyte ions are passed to a mass analysis instrument. In a preferred embodiment an optical fiber butts up against a thin transparent plate on which the specimen resides, with the matrix side in a vacuum acceleration chamber. A mechanical stage moves the specimen in both the x- and y-directions to select a point on the specimen which is to receive the radiation.Type: GrantFiled: January 14, 2000Date of Patent: July 23, 2002Assignee: Sequenom, Inc.Inventors: Franz Hillenkamp, David M. Lough, G. Scott Higgins, Dirk Reuter
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Publication number: 20020042112Abstract: Fast and highly accurate mass spectrometry-based processes for detecting a particular nucleic acid sequence in a biological sample are provided. Depending on the sequence to be detected, the processes can be used, for example, to diagnose a genetic disease or chromosomal abnormality; a predisposition to a disease or condition, infection by a pathogenic organism, or for determining identity or heredity.Type: ApplicationFiled: October 26, 1998Publication date: April 11, 2002Inventors: HUBERT KOSTER, DANIEL P. LITTLE, ANDREAS BRAUN, DAVID M. LOUGH, GUOBING XIANG, DIRK VAN DEN BOOM, CHRISTIAN JURINKE, ANDREAS RUPPERT
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Publication number: 20020005478Abstract: Matrix assisted laser desorption/ionization is performed in a manner to thermalize large analyte ions in a plume of desorbed material for spectroscopic analysis. The thermalized ions have a low or zero mean velocity and are presented at a well-defined instant in time, reducing artifacts and sharpening the spectral peaks. In one embodiment the light is delivered to a matrix or sample holder having a cover, baffle or compartment. The baffle or compartment impedes or contains a plume of desorbed material and the analyte undergoes collisions to lower its mean velocity and directionality. Thus “thermalized” the analyte ions are passed to a mass analysis instrument. In a preferred embodiment an optical fiber butts up against a thin transparent plate on which the specimen resides, with the matrix side in a vacuum acceleration chamber. A mechanical stage moves the specimen in both the x- and y- directions to select a point on the specimen which is to receive the radiation.Type: ApplicationFiled: January 14, 2000Publication date: January 17, 2002Inventors: Franz Hillenkamp, David M. Lough, G. Scott Higgins, Dirk Reuter
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Patent number: 6268131Abstract: A mass spectrometric method for sequencing nucleic acids using RNA polymerases, including DNA-dependent and RNA-dependent RNA polymerases, is provided. The methods use a modified Sanger sequencing strategy in which RNA polymerase is used to generate a set of nested RNA transcripts obtained by base-specific chain termination. These are analyzed by mass spectrometry. A method of identifying transcriptional terminator sequences or attenuator sequences is also provided.Type: GrantFiled: December 15, 1997Date of Patent: July 31, 2001Assignee: Sequenom, Inc.Inventors: Changwon Kang, Young-Soo Kwon, Young Tae Kim, Hubert Köster, Daniel P. Little, Maryanne J. Little, Guobing Xiang, David M. Lough, Charles Cantor
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Patent number: 6133436Abstract: Novel compositions comprised of at least one bead conjugated to a solid support and further conjugated to at least one nucleic acid and preferred methods for making the novel compositions are described. As compared to "flat" surfaces, beads linked to a solid support provide an increased surface area for immobilization of nucleic acids. Furthermore, by selecting a bead with the desired functionality, a practitioner can select a functionalization chemistry for immobilizing nucleic acids, which is different from the chemistry of the solid support.Type: GrantFiled: September 19, 1997Date of Patent: October 17, 2000Assignee: Sequenom, Inc.Inventors: Hubert Koster, David M. Lough
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Patent number: 5900481Abstract: Novel compositions comprised of at least one bead conjugated to a solid support and further conjugated to at least one nucleic acid and preferred methods for making the novel compositions are described. As compared to "flat" surfaces, beads linked to a solid support provide an increased surface area for immobilization of nucleic acids. Furthermore, by selecting a bead with the desired functionality, a practitioner can select a functionalization chemistry for immobilizing nucleic acids, which is different from the chemistry of the solid support.Type: GrantFiled: November 6, 1996Date of Patent: May 4, 1999Assignee: Sequenom, Inc.Inventors: David M. Lough, Hubert Koster
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Patent number: RE41005Abstract: Novel compositions comprised of at least one bead conjugated to a solid support and further conjugated to at least one nucleic acid and preferred methods for making the novel compositions are described. As compared to “flat” surfaces, beads linked to a solid support provide an increased surface area for immobilization of nucleic acids. Furthermore, by selecting a bead with the desired functionality, a practitioner can select a functionalization chemistry for immobilizing nucleic acids, which is different from the chemistry of the solid support.Type: GrantFiled: October 17, 2002Date of Patent: November 24, 2009Assignee: Sequenom, Inc.Inventors: Hubert Koster, David M. Lough
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Patent number: RE44693Abstract: Novel compositions comprised of at least one bead conjugated to a solid support and further conjugated to at least one nucleic acid and preferred methods for making the novel compositions are described. As compared to “flat” surfaces, beads linked to a solid support provide an increased surface area for immobilization of nucleic acids. Furthermore, by selecting a bead with the desired functionality, a practitioner can select a functionalization chemistry for immobilizing nucleic acids, which is different from the chemistry of the solid support.Type: GrantFiled: July 22, 2009Date of Patent: January 7, 2014Assignee: Sequenom, Inc.Inventors: Hubert Köster, David M. Lough