Patents by Inventor David Wenn
David Wenn has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8986927Abstract: A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.Type: GrantFiled: September 3, 2009Date of Patent: March 24, 2015Assignee: The Secretary of State for DefenceInventors: Martin Alan Lee, Hilary Bird, Dario Lyall Leslie, David James Squirrell, John Shaw, David Wenn, Julie Deacon
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Patent number: 7659096Abstract: A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.Type: GrantFiled: July 30, 2007Date of Patent: February 9, 2010Assignee: The Secretary of State for Defence in Her Britannic Majesty's Government of the United Kingdom of Great Britain and Northern IrelandInventors: Martin Alan Lee, Hilary Bird, Dario Lyall Leslie, David James Squirrell, John Shaw, David Wenn, Julie Deacon
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Publication number: 20090325278Abstract: A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.Type: ApplicationFiled: September 3, 2009Publication date: December 31, 2009Applicant: The Secretary of State for DefenceInventors: Martin Alan Lee, Hilary Bird, Dario Lyall Leslie, David James Squirrell, John Shaw, David Wenn, Julie Deacon
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Publication number: 20080176232Abstract: A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.Type: ApplicationFiled: July 30, 2007Publication date: July 24, 2008Applicant: The Secretary of State for DefenceInventors: Martin Alan Lee, Hilary Bird, Dario Lyall Leslie, David James Squirrell, John Shaw, David Wenn, Julie Deacon
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Patent number: 7264950Abstract: A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.Type: GrantFiled: September 29, 2000Date of Patent: September 4, 2007Assignee: The Secretary of State for Defence in Her Britannic Majesty's Government of the United Kingdom of Great Britain and Northern IrelandInventors: Martin Alan Lee, Hilary Bird, Dario Lyall Leslie, David James Squirrell, John Shaw, David Wenn, Julie Deacon
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Patent number: 6564655Abstract: Apparatus for sampling a liquid, said apparatus comprising: i) a sampling device capable of automatically taking a known volume of liquid from a source; ii) fixing means connected to said sampling device and removably attachable to a solid phase extraction (SPE) unit, the fixing means being arranged such that liquid taken from a sample by the sampling device is passed directly through the solid phase extraction unit; iii) a reading device able to read labels on an SPE unit attached to said fixing means; iv) a controller arranged to log codes based upon a signal from the reading device, and thereafter issue instructions to an operator and/or operate the sampling device to take an appropriate volume of liquid in response to a signal from said reading device. Methods of sampling using the apparatus are also claimed. These are useful in, for example analysis methods.Type: GrantFiled: July 5, 2002Date of Patent: May 20, 2003Assignee: Central Research Laboratories LimitedInventors: Malcolm Trayton Austen, John Robert Dodgson, Monica Backes, David Wenn, Richard Lynn Hedgeley, Mark Seymour