Abstract: We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Abstract: Various arrangements of carrier trays and carriers for data storage devices, such as disk drives, are disclosed. The carrier trays and carriers have air flow holes that are carefully arranged to promote good air flow across the data storage devices. In one example, the air holes in a tray are arranged so that, when a data storage device is received on the tray, air passing over a first surface of the base from the forward end can pass through the base, over a part of a second surface and back through the base to flow across the recess typically located under the data storage device.

Abstract: We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Abstract: New biochemical protocols for high throughput processing of mRNA samples into cDNA libraries with adaptor sequences compatible with automated sequencing systems are provided. The provided methods produces cDNA libraries which do not have 3? bias associated with current cDNA library production methods. New methods for the production of DNA libraries from DNA are also provided.

Abstract: The invention is directed to processes for constructing DNA Libraries in which ssDNA containing a chemical modification (CM) at or near the 5?- or 3?-terminus is prepared from a RNA or DNA source, a 1st universal oligonucleotide (Oligo A?) is ligated to the 3?-of the ssDNA, and a 2nd universal oligonucleotide (Oligo B) is ligated to the 5?-terminus of the ssDNA. Chemical modifications useful for the process are functional groups capable of binding a solid support with high affinity, or functional groups that can mediate a non-enzymatic ligation. In one embodiment of the invention, a CM at or near the 5?-terminus of the ssDNA mediates binding of the ssDNA to a solid support, allowing removal of residual unligated Oligo A? prior to ligation of Oligo B. In another embodiment of the invention, a CM at or near the 5?-terminus of ssDNA mediates non-enzymatic ligation of Oligo B to the 5?-terminus of ssDNA, under conditions in which no further ligation of Oligo A? can occur.

Abstract: We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Abstract: A method, system, and computer program product in a logical partitioned data processing system are disclosed for providing a host bridge that implements extended error handling (EEH). If all devices coupled to the host bridge implement EEH, the host bridge is initialized to operate in EEH mode. In EEH mode, the devices handle any error that occurs within the devices without reporting the error to the host bridge. All partitions that share the host bridge continue to operate without being terminated while the devices are handling the error. If at least one device does not implement EEH, the host bridge is initialized to operate in non-EEH mode. In non-EEH mode, a machine check is generated by the host bridge when an error occurs within one of the devices resulting in the termination of all partitions that share the host bridge in response to a receipt of the machine check.

Abstract: We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Abstract: We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a “laboratory-on-a-chip” system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.