Patents by Inventor David Y. Zhang
David Y. Zhang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230399687Abstract: The present invention discloses methods of quantitative multiplex amplicon sequencing system for labeling the original DNA sample with an oligonucleotide barcode sequence by polymerase chain reaction, amplifying the genomic region(s) for high-throughput sequencing and quantifying the sequence in DNA sample. The methods allow analyzing a DNA sample comprising between 1 and 10,000 Target Regions for quantifying potential sequence variants and wildtype molecules.Type: ApplicationFiled: November 1, 2021Publication date: December 14, 2023Applicants: NUPROBE USA, INC., WILLIAM MARSH RICE UNIVERSITYInventors: David Y. ZHANG, Peng DAI, Pengying HAO, Alessandro PINTO
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Publication number: 20140038238Abstract: The present invention relates to assays and kits for carrying out said assays for the rapid, automated detection of infectious pathogenic agents and normal and abnormal genes. The present invention further relates to methods for general amplification of total mRNAs and for analyzing differential mRNA expression using the amplification methods disclosed herein.Type: ApplicationFiled: June 13, 2003Publication date: February 6, 2014Inventor: David Y. Zhang
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Patent number: 8632999Abstract: The present invention relates to assays and kits for carrying out said assays for the rapid, automated detection of infectious pathogenic agents and normal and abnormal genes. The present invention further relates to methods for general amplification of total mRNAs and for analyzing differential mRNA expression using the amplification methods disclosed herein.Type: GrantFiled: June 13, 2003Date of Patent: January 21, 2014Inventor: David Y. Zhang
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Patent number: 7361489Abstract: The present invention relates to assays and kits for carrying out said assays for the rapid, automated detection of infectious pathogenic agents and normal and abnormal genes. The present invention further relates to methods for general amplification of genomic DNA and total mRNAs and for analyzing differential mRNA expression using the amplification methods disclosed herein.Type: GrantFiled: November 21, 2003Date of Patent: April 22, 2008Assignee: Mount Sinai School of Medicine of New York UniversityInventors: David Y. Zhang, Wandi Zhang, Jizu Yi
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Patent number: 6855523Abstract: An improved method allowing for rapid sensitive and standardized detection of a target nucleic acid from a pathogenic microorganism or virus or normal or abnormal gene in a sample is provided. The method involves hybridizing a target nucleic acid to several non-overlapping oligonucleotide probes that hybridize to adjacent regions in the target nucleic acid, the probes being referred to capture/amplification probes and amplification probes, respectively, in the presence of paramagnetic beads coated with a ligand binding moiety. Through the binding of a ligand attached to one end of the capture/amplification probe and the specific hybridization of portions of the probes to adjacent sequences in the target nucleic acid, a complex comprising the target nucleic acid, the probes and the paramagnetic beads is formed. The probes may then ligated together to form a contiguous ligated amplification sequence bound to the beads, which complex may be denatured to remove the target nucleic acid and unligated probes.Type: GrantFiled: December 4, 2002Date of Patent: February 15, 2005Assignee: Mount Sinai School of Medicine of New York UniversityInventors: David Y. Zhang, Margaret Brandwein, Terence C. H. Hsuih
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Publication number: 20040137484Abstract: The present invention relates to assays and kits for carrying out said assays for the rapid, automated detection of infectious pathogenic agents and normal and abnormal genes. The present invention further relates to methods for general amplification of genomic DNA and total mRNAs and for analyzing differential mRNA expression using the amplification methods disclosed herein.Type: ApplicationFiled: November 21, 2003Publication date: July 15, 2004Inventors: David Y. Zhang, Wandi Zhang, Jizu Yi
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Publication number: 20030236205Abstract: The present invention relates to a hybridization signal amplification method (HSAM) that can be used to form nanostructures for use in drug delivery and diagnostics and may comprise molecules aimed at a specific target cell of interest. The nanostuctures may be used to treat infectious diseases and physiological disorders such as proliferative, genetic, neurological or metabolic disorders. The nanostructures of the invention comprise nucleic acid molecules having affinity pairs incorporated into their structure. These affinity pairs are formed from ligand and ligand binding moieties that bind to nucleic acid molecules. This bound entity is a complex, web-like structure that serves as a matrix or framework for delivery of therapeutic or diagnostic agents. Since the nanostructures of the invention are comprised of biocompatible and biodegradable materials, such as nucleic acid molecules and proteins, they provide a safe and easily degradable delivery system.Type: ApplicationFiled: June 21, 2002Publication date: December 25, 2003Inventors: David Y. Zhang, Wandi Zhang
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Publication number: 20030190604Abstract: An improved method allowing for rapid sensitive and standardized detection of a target nucleic acid from a pathogenic microorganism or virus or normal or abnormal gene in a sample is provided. The method involves hybridizing a target nucleic acid to several non-overlapping oligonucleotide probes that hybridize to adjacent regions in the target nucleic acid, the probes being referred to capture/amplification probes and amplification probes, respectively, in the presence of paramagnetic beads coated with a ligand binding moiety. Through the binding of a ligand attached to one end of the capture/amplification probe and the specific hybridization of portions of the probes to adjacent sequences in the target nucleic acid, a complex comprising the target nucleic acid, the probes and the paramagnetic beads is formed. The probes may then ligated together to form a contiguous ligated amplification sequence bound to the beads, which complex may be denatured to remove the target nucleic acid and unligated probes.Type: ApplicationFiled: December 4, 2002Publication date: October 9, 2003Inventors: David Y. Zhang, Margaret Brandwein, Terence C.H. Hsuih
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Publication number: 20030175706Abstract: The present invention relates to assays and kits for carrying out said assays for the rapid, automated detection of infectious pathogenic agents and normal and abnormal genes. The present invention further relates to methods for general amplification of genomic DNA and total mRNAs and for analyzing differential mRNA expression using the amplification methods disclosed herein.Type: ApplicationFiled: October 15, 2001Publication date: September 18, 2003Inventor: David Y. Zhang
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Patent number: 6593086Abstract: The present invention relates to assays and kits for carrying out said assays for the rapid, automated detection of infectious pathogenic agents and normal and abnormal genes. The present invention further relates to methods for general amplification of genomic DNA and total mRNAs and for analyzing differential mRNA expression using the amplification methods disclosed herein.Type: GrantFiled: December 1, 2000Date of Patent: July 15, 2003Assignee: Mount Sinai School of Medicine of New York UniversityInventor: David Y. Zhang
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Patent number: 6569647Abstract: An improved method allowing for rapid sensitive and standardized detection of a target nucleic acid from a pathogenic microorganism or virus or normal or abnormal gene in a sample is provided. The method involves hybridizing a target nucleic acid to several non-overlapping oligonucleotide probes that hybridize to adjacent regions in the target nucleic acid, the probes being referred to capture/amplification probes and amplification probes, respectively, in the presence of paramagnetic beads coated with a ligand binding moiety. Through the binding of a ligand attached to one end of the capture/amplification probe and the specific hybridization of portions of the probes to adjacent sequences in the target nucleic acid, a complex comprising the target nucleic acid, the probes and the paramagnetic beads is formed. The probes may then ligated together to form a contiguous ligated amplification sequence bound to the beads, which complex may be denatured to remove the target nucleic acid and unligated probes.Type: GrantFiled: April 23, 1999Date of Patent: May 27, 2003Assignee: Mount Sinai School of Medicine of New York UniversityInventors: David Y. Zhang, Margaret Brandwein, Terence C. H. Hsuih
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Publication number: 20020182598Abstract: The present invention relates to assays and kits for carrying out said assays for the rapid, automated detection of infectious pathogenic agents and normal and abnormal genes. The present invention further relates to methods for general amplification of genomic DNA and total mRNAs and for analyzing differential mRNA expression using the amplification methods disclosed herein.Type: ApplicationFiled: December 1, 2000Publication date: December 5, 2002Inventor: David Y. Zhang
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Patent number: 5942391Abstract: An improved method allowing for rapid sensitive and standardized detection of a target nucleic acid from a pathogenic microorganism or virus or normal or abnormal gene in a sample is provided. The method involves hybridizing a target nucleic acid to several non-overlapping oligonucleotide probes that hybridize to adjacent regions in the target nucleic acid, the probes being referred to capture/amplification probes and amplification probes, respectively, in the presence of paramagnetic beads coated with a ligand binding moiety. Through the binding of a ligand attached to one end of the capture/amplification probe and the specific hybridization of portions of the probes to adjacent sequences in the target nucleic acid, a complex comprising the target nucleic acid, the probes and the paramagnetic beads is formed. The probes may then ligated together to form a contiguous ligated amplification sequence bound to the beads, which complex may be denatured to remove the target nucleic acid and unligated probes.Type: GrantFiled: July 31, 1996Date of Patent: August 24, 1999Assignee: Mount Sinai School of MedicineInventors: David Y. Zhang, Margaret Brandwein, Terence C. H. Hsuih
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Patent number: 5876924Abstract: An improved method allowing for rapid sensitive and standardized detection of a target nucleic acid from a pathogenic microorganism or virus or normal or abnormal gene in a sample is provided. The method involves hybridizing a target nucleic acid to several non-overlapping oligonucleotide probes that hybridize to adjacent regions in the target nucleic acid, the probes being referred to capture/amplification probes and amplification probes, respectively, in the presence of paramagnetic beads coated with a ligand binding moiety. Through the binding of a ligand attached to one end of the capture/amplification probe and the specific hybridization of portions of the probes to adjacent sequences in the target nucleic acid, a complex comprising the target nucleic acid, the probes and the paramagnetic beads is formed. The probes may then ligated together to form a contiguous ligated amplification sequence bound to the beads, which complex may be denatured to remove the target nucleic acid and unligated probes.Type: GrantFiled: July 31, 1996Date of Patent: March 2, 1999Assignee: Mount Sinai School of MedicineInventors: David Y. Zhang, Margaret Brandwein
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Patent number: RE38442Abstract: An improved method allowing for rapid sensitive and standardized detection of a target nucleic acid from a pathogenic microorganism or virus or normal or abnormal gene in a sample is provided. The method involves hybridizing a target nucleic acid to several non-overlapping oligonucleotide probes that hybridize to adjacent regions in the target nucleic acid, the probes being referred to capture/amplification probes and amplifications probes, respectively, in the presence of paramagnetic beads coated with a ligand binding moiety. Through the binding of a ligand attached to one end of the capture/amplification probe and the specific hybridization of portions of the probes to adjacent sequences in the target nucleic acid, a complex comprising the target nucleic acid, the probes and the paramagnetic beads is formed. The probes may then ligated together to form a contiguous ligated amplification sequence bound to the beads, which complex may be denatured to remove the target nucleic acid and unligated probes.Type: GrantFiled: March 2, 2001Date of Patent: February 24, 2004Assignee: Mount Sinai School of MedicineInventors: David Y. Zhang, Margaret Brandwein