Abstract: Apparatus for depositing a cell product, such as endothelial cell product, in a graft and inserting the graft in a vessel is disclosed. The apparatus preferably comprises a tunneler tube that has a hollow portion for supporting the graft therein, apertures to permit the flow of cell product, and a pointed end cap attached to a distal end of the tunneler tube. The apparatus also has a handle connected to the graft and releaseably connected to a proximal end of the tunneler tube that provides an inlet for cell product and outlet in fluid communication with the lumen of the graft. During deposition, the cell product thus flows through the handle, into the graft and exits through the apertures. During insertion, the tunneler tube is manipulated by the handle to enter a vessel, and is then released from the handle and removed to accommodate anastomoses.

Abstract: The invention is an endothelial cell procurement and deposition kit for collecting fat from a patient, processing said fat to produce an endothelial cell deposition product, and depositing said product on the surface of a graft, all under sterile conditions established and maintained within the components of said kit comprised of: fat collection means for collecting subcutaneous fat from a patient; digestion means connectable to said fat collection means to maintain sterility during reception of said fat and for retaining said fat under sterile conditions during rinsing and digestion to produce a digested product; endothelial cell isolation means connectable to said digestion means for maintaining sterile conditions during reception of said digested product and for separating and isolating microvessel endothelial cells from said digested product to produce an endothelial cell product; cell deposition means connectable to said isolation means for maintaining sterile conditions during reception of said endothel

Abstract: The invention is an endothelial cell procurement and deposition kit for collecting fat from a patient, processing said fat to produce an endothelial cell deposition product, and depositing said product on the surface of a graft, all under sterile conditions established and maintained within the components of said kit comprised of: fat collection means for collecting subcutaneous fat from a patient; digestion means connectable to said fat collection means to maintain sterility during reception of said fat and for retaining said fat under sterile conditions during rinsing and digestion to produce a digested product; endothelial cell isolation means connectable to said digestion means for maintaining sterile conditions during reception of said digested product and for separating and isolating microvessel endothelial cells from said digested product to produce an endothelial cell product; cell deposition means connectable to said isolation means for maintaining sterile conditions during reception of said endothel