Abstract: Methods of screening rinse-off personal care compositions can include the use of explant skin in combination with measurements for moisture and/or cell proliferation.

Abstract: Human skin tissue sample methods and models for identifying and screening test agents as effective for providing skin tone benefits, methods for validating hypotheses for mechanisms driving skin pigmentation as well as methods for driving skin pigment levels in ex-vivo skin tissue. The method includes contacting a cultured human skin tissue sample with a test agent, generating a transcriptional profile from the sample, and comparing the results to a control to determine if the test agent is effective for providing a skin tone benefit.

Abstract: Methods of screening rinse-off personal care compositions can include the use of explant skin in combination with measurements for moisture and/or cell proliferation.

Abstract: Methods and systems for constructing a gene expression signature representative of a number of biological conditions, systems and methods for determining functional relationships between a skin-active agent and skin conditions of interest, and methods and systems for identifying skin-active agents with broad spectrum activity are provided.

Abstract: Methods and systems for constructing a gene expression signature representative of a number of biological conditions, systems and methods for determining functional relationships between a skin-active agent and skin conditions of interest, and methods and systems for identifying skin-active agents with broad spectrum activity are provided.

Abstract: Provided are methods and systems for determining functional relationships between ex vivo skin models and an inflammatory skin condition. Also provided are methods and systems for identifying modulators of inflammation of skin, as well as the use of modulators identified by such methods or systems for the preparation of cosmetic compositions, personal care products, or both.

Abstract: A cosmetic skin care composition for improving the appearance of aging skin. The composition is topically applied to a target area of skin where treatment is desired. The composition includes an effective amount of artichoke leaf extract and carob fruit extract in combination, and is applied for a period of time sufficient to improve the appearance of the aging skin.

Abstract: A cosmetic skin care composition for improving the appearance of aging skin. The composition is topically applied to a target area of skin where treatment is desired. The composition includes an effective amount of artichoke leaf extract and carob fruit extract in combination, and is applied for a period of time sufficient to improve the appearance of the aging skin.

Abstract: A stable skin care composition for improving the appearance of aging skin is disclosed. The stable skin care composition that includes an effective amount of artichoke leaf extract and carob fruit extract that, in combination, can provide a synergistic increase in the up-regulation of key epidermal-associated genes related to skin aging, and thereby provide an improvement in the appearance of aging skin. The stable skin care composition excludes complexing agents and includes sunscreen agents to help photostablize the composition.

Abstract: A screening method for identifying an agent as effective for providing an anti-aging skin benefit is provided. The screening method includes culturing first and second human skin samples from about 3 days to about 19 days, wherein the first and second human skin samples are derived from a human skin surgical waste tissue. The first human skin sample is contacted with at least one test agent. The second human skin sample is contacted with a positive control. Transcriptional profiles are generated from the first and second human skin samples, and the at least one test agent is identified as effective for providing an anti-aging skin benefit when the transcriptional profiles of at least two genes show a similar directional change in comparison to one or more untreated control tissue samples.

Abstract: A stable skin care composition for improving the appearance of aging skin is disclosed. The stable skin care composition that includes an effective amount of artichoke leaf extract and carob fruit extract that, in combination, can provide a synergistic increase in the up-regulation of key epidermal-associated genes related to skin aging, and thereby provide an improvement in the appearance of aging skin. The stable skin care composition is substantially free of complexing agents and includes sunscreen agents to help photostablize the composition.

Abstract: A method of improving the appearance of aging skin that includes applying an effective amount of artichoke leaf extract and olive oil extract in combination to a target area of skin that exhibits a sign of aging skin, and compositions that include an effective amount of artichoke leaf extract and olive oil extract in combination. The composition is applied for a period of time sufficient to improve the appearance of the aging skin.

Abstract: Gene panels, microarrays and biomarker panels relating to genes and gene products associated with age-related oxidative damage to skin, and transcriptional profiling-based methods for identification and evaluation of cosmetic agents for prevention, reversal, or reduction of oxidative damage to skin. Cosmetic agents and compositions comprising the cosmetic agents, capable of inducing nrf2-mediated activation of the antioxidant response element to increase expression of Phase 2 enzymes, methods for restoring optimal redox status to skin employing the agents, and methods for identifying and evaluating cosmetic agents acting via the nrf2-mediated mechanism.

Abstract: A method of improving the appearance of aging skin that includes applying an effective amount of artichoke leaf extract and olive oil extract in combination to a target area of skin that exhibits a sign of aging skin, and compositions that include an effective amount of artichoke leaf extract and olive oil extract in combination. The composition is applied for a period of time sufficient to improve the appearance of the aging skin.

Abstract: A method of improving the appearance of aging skin that includes applying an effective amount of artichoke leaf extract and olive oil extract in combination to a target area of skin that exhibits a sign of aging skin, and compositions that include an effective amount of artichoke leaf extract and olive oil extract in combination. The composition is applied for a period of time sufficient to improve the appearance of the aging skin.

Abstract: Human skin tissue sample methods and models for identifying and screening test agents as effective for providing skin tone benefits, methods for validating hypotheses for mechanisms driving skin pigmentation as well as methods for driving skin pigment levels in ex-vivo skin tissue. The method includes contacting a cultured human skin tissue sample with a test agent, generating a transcriptional profile from the sample, and comparing the results to a control to determine if the test agent is effective for providing a skin tone benefit.

Abstract: Human skin tissue sample methods and models for validating hypotheses for mechanisms driving skin pigmentation as well as methods for driving skin pigment levels in ex-vivo skin tissue. The method includes providing a first cultured human skin tissue sample and testing the effectiveness of the test agent for driving skin pigmentation, and comparing the transcriptional profile generated from the treated human skin tissue sample to the transcriptional profile of a control to validate the hypothesis that the test agent drives skin pigmentation.

Abstract: A method of improving the appearance of a hyperpigmented spot may comprise the step of applying a composition comprising an effective amount of banyan tree serum fraction, lotus serum fraction, and clover serum fraction to a hyperpigmented spot on a skin surface, wherein the composition is applied for a period of time sufficient to improve the appearance of the hyperpigmented spot. The method may include the step of identifying a hyperpigmented spot on a facial skin surface. Other methods as disclosed include a method for improving the appearance of post-inflammatory hyperpigmentation.

Abstract: A screening method for identifying an agent as effective for providing an anti-aging skin benefit is provided. The screening method includes culturing first and second human skin samples from about 3 days to about 19 days, wherein the first and second human skin samples are derived from a human skin surgical waste tissue. The first human skin sample is contacted with at least one test agent. The second human skin sample is contacted with a positive control. Transcriptional profiles are generated from the first and second human skin samples, and the at least one test agent is identified as effective for providing an anti-aging skin benefit when the transcriptional profiles of at least two genes show a similar directional change in comparison to one or more untreated control tissue samples.

Abstract: A method of improving the appearance of aging skin may comprise the step of applying a composition comprising an effective amount of banyan tree serum fraction, lotus serum fraction, and clover serum fraction to a sign of aging on a skin surface, wherein the composition is applied for a period of time sufficient to improve the appearance of the aging skin. The method may include the step of identifying a sign of aging on a facial skin surface.