Abstract: An electroporation apparatus has an electroporation probe terminals for linking with electrodes. A foam is injected at the treatment site to displace blood rather than mixing with it, increasing the contact time of a higher concentration of active agent with the tissue and thus resulting in greater efficacy. With foam solutions, a lower concentration of agent can be used to obtain the same therapeutic effect as in their liquid counterpart, reducing the prevalence of side effects associated with higher concentrations. A foam solution compared to an equivalent liquid solution enables more efficient cell electroporation particularly where bipolar pulses have been employed by mitigating an increase in tissue conductivity as would normally be observed with a comparable liquid solution. A more efficient cell permeabilisation would result in better results where electroporation is being delivered alone or as a tool to aid in the uptake of molecules into the cell.

Abstract: An electroporation apparatus has an electroporation probe terminals for linking with electrodes. A foam is injected at the treatment site to displace blood rather than mixing with it, increasing the contact time of a higher concentration of active agent with the tissue and thus resulting in greater efficacy. With foam solutions, a lower concentration of agent can be used to obtain the same therapeutic effect as in their liquid counterpart, reducing the prevalence of side effects associated with higher concentrations. A foam solution compared to an equivalent liquid solution enables more efficient cell electroporation particularly where bipolar pulses have been employed by mitigating an increase in tissue conductivity as would normally be observed with a comparable liquid solution. A more efficient cell permeabilization would result in better results where electroporation is being delivered alone or as a tool to aid in the uptake of molecules into the cell.

Abstract: An electroporation apparatus has an electroporation probe terminals for linking with electrodes. A foam is injected at the treatment site to displace blood rather than mixing with it, increasing the contact time of a higher concentration of active agent with the tissue and thus resulting in greater efficacy. With foam solutions, a lower concentration of agent can be used to obtain the same therapeutic effect as in their liquid counterpart, reducing the prevalence of side effects associated with higher concentrations. A foam solution compared to an equivalent liquid solution enables more efficient cell electroporation particularly where bipolar pulses have been employed by mitigating an increase in tissue conductivity as would normally be observed with a comparable liquid solution. A more efficient cell permeabilization would result in better results where electroporation is being delivered alone or as a tool to aid in the uptake of molecules into the cell.

Abstract: An electroporation probe has a housing supporting needles electrically linked to a coupler for an electrical drive. A cap has apertures in a distal face and aligned with the needles. The cap is movable longitudinally against a spring bias relative to the needles to allow insertion by the needles in use as the cap is pressed against patient tissue. There is a limit feature for abutting a limiter setting maximum extent of longitudinal movement relative to the housing. This allows the probe to be presented by the physician without the needles protruding and for them to be inserted up to the user-set maximum extent with maintenance of spacings due to the guiding action of the cap pressed against the tissue surface.

Abstract: The present invention relates to a method for the treatment of cancer in a subject which comprises the steps of: (i) administering electroporation to a tumour in the subject; and (II) administering a T or B-cell activiating agent to the subject, wherein step (i) and (II) may be performed in either order.

Abstract: The present invention relates to a method for the treatment of cancer in a subject which comprises the steps of: (i) administering electroporation to a tumour in the subject; and (II) administering a T or B-cell activiating agent to the subject, wherein step (i) and (II) may be performed in either order.

Abstract: The present invention relates to a method for the treatment of cancer in a subject which comprises the steps of: (i) administering electroporation to a tumour in the subject; and (ii) administering a T or B-cell activating agent to the subject, wherein step (i) and (ii) may be performed in either order.

Abstract: A thoracoscopic electroporation device for carrying out electroporation on tissue includes a suction head and a plurality of needle electrodes. The needle electrodes include a central needle providing a first electrode and a plurality of second needles which are spaced-apart around the periphery of the suction head. The electrodes are movable from a retracted configuration to a deployed configuration and in the deployed configuration the needles extend from the suction head. The suction head has a retracted delivery configuration and an expanded deployed configuration. The suction head includes a suitable flexible material for biasing the suction head into the expanded deployed configuration. The suction head has channels for the needles and the needles are movable through the channels from the retracted to the deployed configuration. In the deployed configuration, the central first needle extends beyond the peripheral needles.

Abstract: The present invention relates to a method for the treatment of cancer in a subject which comprises the steps of: (i) administering electroporation to a tumour in the subject; and (ii) administering a T or B-cell activating agent to the subject, wherein step (i) and (ii) may be performed in either order.

Abstract: An apparatus for use in carrying out a prophylactic or treatment procedure on tissue comprises a head piece 1 having a receiver 2 for receiving an instrument such as an endoscope 10 and chamber 3 into which tissue is drawn by vacuum. There are two electrodes 11 within the chamber 2 and two electrodes 25 located on opposite sides of the entrance 5 into the chamber. The voltage applied to the inner and outer electrodes 11, 25 can be varied independently an allows tissue underlying the device to be treated. This is particularly important for colorectal cancers.

Abstract: A thoracoscopic electroporation device for carrying out electroporation on tissue includes a suction head and a plurality of needle electrodes. The needle electrodes include a central needle providing a first electrode and a plurality of second needles which are spaced-apart around the periphery of the suction head. The electrodes are movable from a retracted configuration to a deployed configuration and in the deployed configuration the needles extend from the suction head. The suction head has a retracted delivery configuration and an expanded deployed configuration. The suction head includes a suitable flexible material for biasing the suction head into the expanded deployed configuration. The suction head has channels for the needles and the needles are movable through the channels from the retracted to the deployed configuration. In the deployed configuration, the central first needle extends beyond the peripheral needles.

Abstract: The present invention provides a non-viral vector which comprises a sequence encoding an RNA replicase and a nuclear localisation sequence. The vector may also comprise a nucleotide sequence of interest (NOI). The vector may be used to deliver an NOI to a target cell.

Abstract: Apparatus (20) for carrying out a prophylactic or treatment procedure on tissue comprises a device (21) having a chamber (22) and at least one active element such as an electrode (23) within the chamber (22). The chamber (22) has an opening (26) through which tissue is drawn into the chamber (22). Means for drawing the tissue into the chamber may comprise a vacuum lumen with vacuum orifices (25) in the chamber (22). Treatment such as electroporation may be applied to tissue in the chamber (22).

Abstract: An apparatus for use in carrying out a prophylactic or treatment procedure on tissue comprises a head piece 1 having a receiver 2 for receiving an instrument such as an endoscope 10 and chamber 3 into which tissue is drawn by vacuum. There are two electrodes 11 within the chamber 2 and two electrodes 25 located on opposite sides of the entrance 5 into the chamber. The voltage applied to the inner and outer electrodes 11, 25 can be varied independently an allows tissue underlying the device to be treated. This is particularly important for colorectal cancers.

Abstract: Apparatus (20) for carrying out a prophylactic or treatment procedure on tissue comprises a device (21) having a chamber (22) and at least one active element such as an electrode (23) within the chamber (22). The chamber (22) has an opening (26) through which tissue is drawn into the chamber (22). Means for drawing the tissue into the chamber may comprise a vacuum lumen with vacuum orifices (25) in the chamber (22). Treatment such as electroporation may be applied to tissue in the chamber (22).