Patents by Inventor Denis Drapeau
Denis Drapeau has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9012180Abstract: An improved system for large scale production of polypeptides in cell culture is provided. In accordance with the present invention, cells expressing a polypeptide of interest are grown in media that contain copper, glutamate or both. The use of such a system allows production of polypeptides in which misfolding and/or aggregation are reduced, and in which total glycosylation is increased. Polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, agricultural or other commercial compositions.Type: GrantFiled: February 29, 2008Date of Patent: April 21, 2015Assignee: Wyeth LLCInventors: Denis Drapeau, Jessica Snow, Gregory Hiller, Yen Tung Luan
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Publication number: 20140322758Abstract: The present invention provides, among other things methods of increasing cell density, viability and/or titer in a cell culture including steps of adding a composition comprising iron to the cell culture.Type: ApplicationFiled: October 9, 2012Publication date: October 30, 2014Inventors: Wenge Wang, Yen-Tung Luan, Denis Drapeau
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Publication number: 20130084593Abstract: This invention relates to methods for rationally designing cell culture media for use in cell cultures, e.g., cell cultures employed in polypeptide production; cell culture media designed with the disclosed methods; methods of producing a polypeptide of interest, e.g., an antibody, using such media; polypeptides produced using the methods and media disclosed herein; and pharmaceuticals compositions containing such polypeptides. The rationally designed media contain a concentration of an amino acid that is calculated for use in cell mass, a concentration of the amino acid that is calculated for use in cell maintenance, and a concentration of the amino acid that is calculated for incorporation into the polypeptide of interest.Type: ApplicationFiled: July 30, 2012Publication date: April 4, 2013Applicant: Wyeth LLCInventors: Yen-Tung Luan, Wenge Wang, Ryan Nolan, Denis Drapeau
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Patent number: 8232075Abstract: This invention relates to methods for rationally designing cell culture media for use in cell cultures, e.g., cell cultures employed in polypeptide production; cell culture media designed with the disclosed methods; methods of producing a polypeptide of interest, e.g., an antibody, using such media; polypeptides produced using the methods and media disclosed herein; and pharmaceuticals compositions containing such polypeptides. The rationally designed media contain a concentration of an amino acid that is calculated for use in cell mass, a concentration of the amino acid that is calculated for use in cell maintenance, and a concentration of the amino acid that is calculated for incorporation into the polypeptide of interest.Type: GrantFiled: November 8, 2007Date of Patent: July 31, 2012Assignee: Wyeth LLCInventors: Yen-Tung Luan, Wenge Wang, Ryan Nolan, Denis Drapeau
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Patent number: 8039231Abstract: Methods and processes for improved recombinant protein production are provided. The methods are useful for production of growth factors, particularly those of the TGF-? superfamily, including bone morphogenetic proteins (BMPs), such as BMP-2. Suitable host cells are cultured in media where iron is present at a concentration of at least 2.25 ?M and if pyridoxal is present, it makes up less than 55% of the molar concentration of vitamin B6 in the media.Type: GrantFiled: April 16, 2009Date of Patent: October 18, 2011Assignee: Wyeth LLCInventors: Yen-Tung Luan, Wenge Wang, Gregg Nyberg, Jose Manuel Gomes, Denis Drapeau, Terry Cardoza
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Publication number: 20090317867Abstract: Methods and processes for improved recombinant protein production are provided. The methods are useful for production of growth factors, particularly those of the TGF-? superfamily, including bone morphogenetic proteins (BMPs), such as BMP-2. Suitable host cells are cultured in media where iron is present at a concentration of at least 2.25 ?M and if pyridoxal is present, it makes up less than 55% of the molar concentration of vitamin B6 in the media.Type: ApplicationFiled: April 16, 2009Publication date: December 24, 2009Applicant: Wyeth, Madison, New JerseyInventors: Yen-Tung Luan, Wenge Wang, Gregg Nyberg, Jose Manuel Gomes, Denis Drapeau, Terry Cardoza
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Publication number: 20090068705Abstract: An improved system for large scale production of polypeptides in cell culture is provided. In accordance with the present invention, cells expressing a polypeptide of interest are grown in media that contain copper, glutamate or both. The use of such a system allows production of polypeptides in which misfolding and/or aggregation are reduced, and in which total glycosylation is increased. Polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, agricultural or other commercial compositions.Type: ApplicationFiled: February 29, 2008Publication date: March 12, 2009Applicant: WyethInventors: Denis Drapeau, Jessica Snow, Gregory Hiller, Yen Tung Luan
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Patent number: 7429491Abstract: Methods of improving protein production in animal cell cultures are provided. Cell culture methods are presented wherein glucose is fed in a restricted manner to cell culture; this restricted feeding of glucose to the cell culture results in lactate production being controlled to a low level. The restricted feeding of glucose in a fed-batch process is not accomplished through a constant-rate feeding of glucose, and the restricted feeding need not depend on sampling. Instead, restricted feeding of glucose to the culture is accomplished through feeding of glucose to the culture at a rate that is a function of an expected or a premodeled rate of glucose consumption by the animal cells when exposed to medium containing a high level of glucose. Because lactate production is controlled to low levels, recombinant protein production is increased.Type: GrantFiled: May 17, 2004Date of Patent: September 30, 2008Assignee: WyethInventors: Yen-Tung Luan, Terry Cardoza Stanek, Denis Drapeau
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Publication number: 20080108553Abstract: This invention relates to methods for rationally designing cell culture media for use in cell cultures, e.g., cell cultures employed in polypeptide production; cell culture media designed with the disclosed methods; methods of producing a polypeptide of interest, e.g., an antibody, using such media; polypeptides produced using the methods and media disclosed herein; and pharmaceuticals compositions containing such polypeptides. The rationally designed media contain a concentration of an amino acid that is calculated for use in cell mass, a concentration of the amino acid that is calculated for use in cell maintenance, and a concentration of the amino acid that is calculated for incorporation into the polypeptide of interest.Type: ApplicationFiled: November 8, 2007Publication date: May 8, 2008Inventors: Yen-Tung Luan, Wenge Wang, Ryan Nolan, Denis Drapeau
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Patent number: 7335491Abstract: An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided.Type: GrantFiled: August 25, 2005Date of Patent: February 26, 2008Assignee: Wyeth Research Ireland LimitedInventors: Denis Drapeau, Yen-Tung Luan, James R. Mercer, Wenge Wang, Daniel R. Lasko
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Patent number: 7300773Abstract: An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided.Type: GrantFiled: August 25, 2005Date of Patent: November 27, 2007Assignee: Wyeth Research Ireland LimitedInventors: Denis Drapeau, Yen-Tung Luan, James R. Mercer, Wenge Wang, Daniel R. Lasko
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Patent number: 7294484Abstract: An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided.Type: GrantFiled: August 25, 2005Date of Patent: November 13, 2007Assignee: Wyeth Research Ireland LimitedInventors: Denis Drapeau, Yen-Tung Luan, James R. Mercer, Wenge Wang, Daniel R. Lasko
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Publication number: 20070110743Abstract: Methods of producing a protein in cell culture comprising an anti-senescence compound, such as the antioxidant carnosine, are provided. According to teachings of the present invention, cells grown in a cell culture medium comprising an anti-senescence compound exhibit increased viability and productivity. Furthermore, cell cultures grown in the presence of an anti-senescence compound exhibit decreased levels of high molecular weight aggregates in the cell culture medium.Type: ApplicationFiled: October 24, 2006Publication date: May 17, 2007Applicant: WyethInventors: Denis Drapeau, Yen-Tung Luan, Paul Thoday, Wenge Wang, Judy Chou
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Publication number: 20060160180Abstract: An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided.Type: ApplicationFiled: August 25, 2005Publication date: July 20, 2006Applicant: Wyeth Research Ireland LimitedInventors: Denis Drapeau, Yen-Tung Luan, James Mercer, Wenge Wang, Daniel Lasko
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Publication number: 20060121568Abstract: An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided.Type: ApplicationFiled: August 25, 2005Publication date: June 8, 2006Applicant: Wyeth Research Ireland LimitedInventors: Denis Drapeau, Yen-Tung Luan, James Mercer, Wenge Wang, Daniel Lasko
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Publication number: 20060121569Abstract: An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided.Type: ApplicationFiled: August 25, 2005Publication date: June 8, 2006Applicant: Wyeth Research Ireland LimitedInventors: Denis Drapeau, Yen-Tung Luan, James Mercer, Wenge Wang, Daniel Lasko
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Publication number: 20050070013Abstract: Methods of improving protein production in animal cell cultures are provided. Cell culture methods are presented wherein glucose is fed in a restricted manner to cell culture; this restricted feeding of glucose to the cell culture results in lactate production being controlled to a low level. The restricted feeding of glucose in a fed-batch process is not accomplished through a constant-rate feeding of glucose, and the restricted feeding need not depend on sampling. Instead, restricted feeding of glucose to the culture is accomplished through feeding of glucose to the culture at a rate that is a function of an expected or a premodeled rate of glucose consumption by the animal cells when exposed to medium containing a high level of glucose. Because lactate production is controlled to low levels, recombinant protein production is increased.Type: ApplicationFiled: May 17, 2004Publication date: March 31, 2005Inventors: Yen-Tung Luan, Terry Stanek, Denis Drapeau
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Patent number: 5830761Abstract: Cell culture media are provided containing high L-cystine concentration and low L-glutamic acid concentration. The media are useful for recombinant production of proteins using mammalian cell cultures.Type: GrantFiled: June 7, 1995Date of Patent: November 3, 1998Assignee: Genetics Institute, Inc.Inventors: Denis Drapeau, S. Robert Adamson, Yen-Tung Luan, Paul Thoday
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Patent number: D467178Type: GrantFiled: January 23, 2002Date of Patent: December 17, 2002Inventor: Denis Drapeau
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Patent number: H1532Abstract: Methods and nutrient media are disclosed useful for adapting mammalian cell lines to culture at increased cell densities.Type: GrantFiled: November 3, 1993Date of Patent: May 7, 1996Assignee: Genetics Institute, Inc.Inventors: S. Robert Adamson, Denis Drapeau, Yen-Tung Luan, Douglas A. Miller