Abstract: Devices, systems, kits, and methods used to collect drainage fluid from a patient are disclosed. The systems or kits include a multi-port manifold, and one or more drainage tubes to fluidly connect vacuum drainage bottles with the multi-port manifold. One or more vacuum drainage bottles can also be included in the kit as desired. The method of drainage fluid collection can include filling two vacuum drainage bottles simultaneously with drainage fluid, then filling a third vacuum drainage bottle with drainage fluid, and then filling a fourth vacuum drainage bottle with drainage fluid.