Abstract: Method for the determination of a component in a liquid which component is selected from ions, polar substances lipophilic substances, which comprises contacting a liquid sample containing said component with, and allowing same to act upon, a complex ligand or host molecule which is selective toward the component, said complex ligand or host molecule being bonded to a chromophore material, and measuring the change of extinction or the wavelength displacement of the resulting mixture as a measure of the initial content of said component; corresponding reagent test kits are also provided. The complex ligands or host molecules include cyclodextrins, cyclophanes, crown ethers, cryptands, podands valinomycin, gramacidin and monactin.

Abstract: Biologically active proteins are immobilized onto a carrier by reacting a compound containing at least two secondary amino groups at a temperature of from -80.degree. to +50.degree. C with a chlorination agent with the conversion of the secondary amino groups into iminochloride groups of the formula ##STR1## in which R and R.sub.1, which can be the same or different, are aliphatic, aromatic or aliphatic-aromatic radicals, and reacting the carrier material product thus obtained(a) directly with a biologically-active protein, in an aqueous solvent, or(b) with a bifunctional compound containing at least one primary amino group, optionally in an aqueous or organic solvent, and reacting the product thereby obtained containing groups of the formula ##STR2## in which R.sub.2 is an alkylene radical, an .alpha.,.omega.

Abstract: Carrier-bound macromolecular compounds, especially bioactive macromolecular compounds, are produced by reacting a macromolecular compound A with a coupling compound B containing at least one reactive function capable of coupling with compound A and at least one further reactive function capable of polymerization, adding to the resulting coupled reaction product AB an unswollen molecular sieve material having cross-linking sufficient to exclude penetration by compound A, and polymerizing the polymerizable group of the coupled product AB in the molecular sieve material to form a polymer body in the molecular sieve material and fix compound A on the surface of the molecular sieve material. Compound A can be, e.g., an enzyme, antibody or hormone, and coupling compound B typically contains, as the coupable function, an alkylation or acylation group such as oxirane, ethyleneimine, halide, acid halide, azide or anhydride groups, and, as the polymerizable function, a carbon-carbon or carbon-oxygen double bond.

Abstract: Biologically active proteins are bound onto an insoluble carrier by reacting such protein in an aqueous phase with a carrier comprising an activated polysaccharide having a hydrophilic graft co-polmyer grafted thereinto.

Abstract: Carrier-bound proteins are prepared by reacting a protein in aqueous solution with a coupling compound having at least one group capable of acylating or alkylating proteins and at least one additional group capable of producing a bond with a carrier material, and reacting the resulting product with a carrier material, optionally forming the carrier material in situ by polymerization of a monomer or monomer mixture in the presence of said product.

Abstract: Glucose is converted into gluconic acid by oxidation with oxygen in aqueous solution, by a process comprising passing a glucose containing aqueous solution over a catalyst which contains glucose oxidase and catalase, bound to an appropriate carrier, in immediate proximity to one another. The process avoids the disadvantage of previous catalytic processes depending on catalysts which rapidly lose their activity and is adapted to large-scale operation.