Abstract: A method of determining a color transformation for images of biological material includes preparing a first set of biological test objects using a first preparation method, and preparing a second set of biological test objects using a second preparation method. Each test object in the second set of test objects corresponding to a counterpart test object in the first set of test objects, the test object and its counterpart being of the same biological type of material. For each test object in the first and second set of test objects, the color of the test object is determined thereby generating a first and second set of colors The method further includes generating a conversion table indicating a mapping between the colors in the first set of colors and the corresponding colors in the second set of colors. The first and second preparation methods include first and second staining methods, respectively.

Abstract: A method of determining a colour transformation for images of biological material comprises—preparing a first set (100) of biological test objects (101-105) using a first preparation method, the set comprising at least one test object;—preparing a second set (130) of biological test objects (131-135) using a second preparation method, each test object (131) in the second set of test objects corresponding to a counterpart test object (101) in the first set of test objects, the test object and its counterpart being of the same biological type of material;—for each test object (101) in the first set of test objects, determining a colour (111) of the test object, thereby generating a first set (110) of colours (111-115);—for each test object (131) in the second set of test objects, determining a colour (121) of the test object, thereby generating a second set (120) of colours (121-125),—generating a conversion table (140) indicating a mapping between the colours in the first set (110) of colours and the correspon

Abstract: The invention relates to a spot generator (10) having: —an entrysurface (12) for receiving an incident light beam (20) and an exit surface (14) for (22, 24) transmitting the light beam, the entry surface defining an entryside (16) and the exit surface defining an exit side (18). According to the invention, the spot generator is designed to modulate the incident light beam to generate on the exit side a first plurality (22) and a second plurality (24) of separate light spots, each light spot belonging to the first plurality having a first angular spectrum and each light spot belonging to the second plurality having a second angular spectrum different than the first angular spectrum. Advantageously, the spot generator comprises a periodic binary phase structure. The invention further relates to a multi-spot scanning microscope and to a method of imaging a microscopic sample.

Abstract: The invention relates to a spot-generator (10) having: an entrance surface (12) for receiving an incident light beam (20) and an exit surface (14) for transmitting the light beam, the entrance surface defining an entrance side (16) and the exit surface defining an exit side (18), wherein the spot generator is designed to modulate the incident light beam to generate on the exit side a plurality of separate light spots. According to the invention, the plurality of light spots comprises a first light spot (22) generated in a first focal plane (24) and a second light spot (26) generated in a second focal plane (28), the first focal plane and the second focal plane being essentially perpendicular to the mean propagation direction of the exit light beam, and wherein the first light spot (22) differs from every other light spot generated on the exit side by the spot generator in the projection of its position on a plane essentially perpendicular to the mean propagation direction of the exit light beam.