Abstract: Provided is a method for quantifying sugar appearing in a biological fluid of a subject, the method include (a) obtaining a biological sample from a subject, who has ingested one or more sugars, over a period of 0 to 24 hours after the ingesting; and (b) measuring the amount of the one or more sugars in the biological sample with an organoborane compound coupled to a fluorophore. Also provided are assays and kits for performing the above methods.

Abstract: Disclosed are systems for measuring the concentration of a disaccharide in an aqueous medium, the system comprising a boronic acid derivative and a fluorescent molecule. Also disclosed are methods of determining gastrointestinal permeability in a subject, the method comprising contacting an aqueous medium obtained from a subject with the above system to obtain a mixture, where the patient has consumed a known quantity of a disaccharide; measuring the fluorescence emission of the mixture; and correlating the extent of fluorescence emission to the concentration of the disaccharide in the aqueous medium; and correlating the concentration of the disaccharide in the aqueous medium to the gastrointestinal permeability in the subject.

Abstract: The present disclosure relates to a method for quantifying sugar appearing in a biological fluid of a subject, the method comprising: (a) obtaining a biological sample from a subject, who has ingested one or more sugars, over a period of 0 to 24 hours after the ingesting; and (b) measuring the amount of the one or more sugars in the biological sample with an organoborane compound coupled to a fluorophore. Disclosed are also assays and kits for performing the above methods.

Abstract: Disclosed are systems for measuring the concentration of a disaccharide in an aqueous medium, the system comprising a boronic acid derivative and a fluorescent molecule. Also disclosed are methods of determining gastrointestinal permeability in a subject, the method comprising contacting an aqueous medium obtained from a subject with the above system to obtain a mixture, where the patient has consumed a known quantity of a disaccharide; measuring the fluorescence emission of the mixture; and correlating the extent of fluorescence emission to the concentration of the disaccharide in the aqueous medium; and correlating the concentration of the disaccharide in the aqueous medium to the gastrointestinal permeability in the subject.