Abstract: A method for impregnating a fibrous preform by injection or infusion, comprising steps of: —providing a mold having at least two portions, said mold presenting an inlet orifice and a vent orifice placed on opposite sides of the mold; —placing a fibrous preform in a portion of the mold; —placing a thermo-expandable seal in a passage formed between an inside wall of the mold and the fibrous preform, said passage extending substantially along the direction (X) extending between the opposite sides of the mold; —closing the mold; —heating the mold so that the thermo-expandable seal expands and shuts off said passage; and—impregnating the fibrous preform with an impregnation material.

Abstract: A method for impregnating a fibrous preform by injection or infusion, comprising steps of: —providing a mold having at least two portions, said mold presenting an inlet orifice and a vent orifice placed on opposite sides of the mold; —placing a fibrous preform in a portion of the mold; —placing a thermo-expandable seal in a passage formed between an inside wall of the mold and the fibrous preform, said passage extending substantially along the direction (X) extending between the opposite sides of the mold; —closing the mold; —heating the mold so that the thermo-expandable seal expands and shuts off said passage; and —impregnating the fibrous preform with an impregnation material.

Abstract: Nucleic sequences of plasmid origin are isolated from bacteria of the enterohaemorrhagic Escherichia coli group (EHEC) and used for the identification of EHEC(s), especially those possessing the genes encoding the virulence factors enterohaemolysin and intimin, and more particularly the specific detection of serotype O157:H7, and in detection kits.

Abstract: Nucleic sequences of plasmid origin are isolated from bacteria of the enterohaemorrhagic Escherichia coli group (EHEC) and used for the identification of EHEC(s), especially those possessing the genes encoding the virulence factors enterohaemolysin and intimin, and more particularly the specific detection of serotype O157:H7, and in detection kits.

Abstract: The invention concerns nucleic sequences of plasmid origin, present in bacteria of the group enterohemorrhagic Escherichia coli (EHEC), the use of said sequences for searching for EHEC, in particular those having genes coding for enterohemolysin and intimin virulence factors, and more particularly for specific detection of the O157:H7 serotype. The invention also concerns a method using said sequences and detection kits containing them.

Abstract: Fragments of nucleic acids derived from an appropriate mycobacteria genome, particularly Mycobacterium tuberculosis, their applications in the diagnosis of mycobacteria infections, as well as plasmides containing said fragments. The nucleotidic sequence is comprised of a nucleotidic sequence repeated in the genome of a mycobacterium and specific of the bacillus of tuberculosis and is characterized by a strong hybridation with M. tuberculosis.

Abstract: Fragments of nucleic acids derived from an appropriate mycobacteria genome, particularly Mycobacterium tuberculosis, their applications in the diagnosis of mycobacteria infections, as well as plasmides containing said fragments. The nucleotidic sequence is comprised of a nucleotidic sequence repeated in the genome of a mycobacterium and specific of the bacillus of tuberculosis and is characterized by a strong hybridation with M. tuberculosis.

Abstract: The invention relates to a nucleic acid fragment derived from the Mycobacterium tuberculosis genome, characterized in that it contains one of the sequences I, II, III and IV, defined in the following manner:I: a sequence chosen from one of the sequences A to H:A: 5'-CCCGCGGCAAAGCCCGCAGGACCACGATCG-3' (SEQ ID NO. 1)B: 5'-CGACCCGCCAGCCCAGGATCCTGCGAGCGT-3' (SEQ ID NO. 2)C: 5'-GGCGGGTCCAGATGGCTTGCTCGATCGCGT-3' (SEQ ID NO. 3)D: 5'-GTTGGCGGGTCCAGATGGCTTGCTCGATCG-3' (SEQ ID NO. 4)E: 5'-TCAAAGGGTTTGACAAATTAATGATTGGTC-3' (SEQ ID NO. 5)F: 5'-TCGTGTACAAAATGTGGACAAGTA-3' (SEQ ID NO. 6)G: 5'-TCGACGGACGTCGTGACCAGAAGTC-3' (SEQ ID NO. 7)H: 5'-GTCGACACGCCTTCTGCACGGGAAGTCCTT-3' (SEQ ID NO.

Abstract: A nucleic acid sequence which specifically hybridizes with a genomic nucleic acid sequence of strains of the complex Mycobacterium avium-intracellulare selected from nucleotide sequence SEQ ID No 1, nucleotide sequence SEQ ID No 2, complementary sequences thereof, and sequences differing therefrom by the mutation, insertion, deletion or substitution of one or more bases, but does not hybridize with the nucleic acids of mycobacteria which do not belong to said complex. Fragments of the sequence may be used as primers for amplifying sequences which are specific for strains of the complex Mycobacterium avium-intracellulare, and as nucleic probes which are specific for nucleic sequences of strains of the complex Mycobacterium avium-intracellulare. A method for detecting strains of the complex Mycobacterium avium-intracellulare in a biological sample, and a kit for implementing the method, are also provided.

Abstract: Nucleic acid fragments have been obtained from the genome of mycobacteria, and applications of the nucleic acid fragments in the diagnosis of mycobacterial infections are described. More particularly, the present invention concerns an isolated polynucleotide of the formula:5'-(SEQ ID NO: 1)-(formula III)-(SEQ ID NO: 2)-3'where formula III represents a polynucleotide containing nucleotides 343-1152 of SEQ ID NO: 3. Primers and probes based on the isolated polynucleotide, DNA complementary to any of the polynucleotides, primers or probes, a method of detecting and identifying at least one species or group of mycobacteria, and a kit, box, or coordinated set for conducting the method are also described.