Patents by Inventor Don A. Masquelier

Don A. Masquelier has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for controlling the growth of prokaryotic and eukaryotic cells
    Patent number: 10883077
    Abstract: The present disclosure relates to methods for control of cell growth rates where cell growth is measured in situ. The methods are applicable to bacterial cells, mammalian cells, non-mammalian eukaryotic cells, plant cells, yeast cells, fungi, and archea.
    Type: Grant
    Filed: March 31, 2020
    Date of Patent: January 5, 2021
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier
  • FLOW THROUGH ELECTROPORATION MODULES AND INSTRUMENTATION
    Publication number: 20200399579
    Abstract: The present disclosure provides a flow-through electroporation device configured for use in an automated multi-module cell processing environment and configured to decrease cell processing time and the risk of clogging.
    Type: Application
    Filed: June 17, 2020
    Publication date: December 24, 2020
    Inventors: Nathan Bade, Jorge Bernate, Phillip Belgrader, Don Masquelier
  • AUTOMATED CELL PROCESSING INSTRUMENTS COMPRISING REAGENT CARTRIDGES
    Publication number: 20200398280
    Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment.
    Type: Application
    Filed: September 4, 2020
    Publication date: December 24, 2020
    Inventors: Jorge Bernate, Don Masquelier, Phillip Belgrader, Brian Van Hatten, Bruce Chabansky
  • ELECTROPORATION CUVETTES FOR AUTOMATION
    Publication number: 20200399658
    Abstract: The present disclosure relates to an electroporation device that may include many electroporation units and electroporation systems that can be used in an automated environment, e.g., as one station or module in a multi-station or multi-module cell processing environment.
    Type: Application
    Filed: September 4, 2020
    Publication date: December 24, 2020
    Inventors: Jorge Bernate, Phil Belgrader, Don Masquelier, Vlorent Morina
  • INSTRUMENTS, MODULES, AND METHODS FOR IMPROVED DETECTION OF EDITED SEQUENCES IN LIVE CELLS
    Publication number: 20200399584
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Application
    Filed: September 4, 2020
    Publication date: December 24, 2020
    Inventors: Phillip Belgrader, Don Masquelier, Andrew Garst, Richard Fox
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10851339
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: September 4, 2020
    Date of Patent: December 1, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Andrew Garst, Richard Fox
  • Modification of cells by introduction of exogenous material
    Patent number: 10851389
    Abstract: The present disclosure provides methods and devices for rapid and efficient modification of a variety of cell types, including mammalian cells, plant cells, archaea, yeasts, and bacteria, by novel methods of introducing exogenous materials, e.g. nucleic acids.
    Type: Grant
    Filed: August 26, 2019
    Date of Patent: December 1, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10844344
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: March 16, 2020
    Date of Patent: November 24, 2020
    Assignee: Inscripta, Inc.
    Inventors: Andrew Garst, Richard Fox, Phillip Belgrader, Don Masquelier
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10835869
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: July 14, 2020
    Date of Patent: November 17, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Matthew Estes, Bruce Chabansky, Andrew Garst, Richard Fox, Jorge Bernate
  • INSTRUMENTS, MODULES, AND METHODS FOR IMPROVED DETECTION OF EDITED SEQUENCES IN LIVE CELLS
    Publication number: 20200346168
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Application
    Filed: July 14, 2020
    Publication date: November 5, 2020
    Inventors: Phillip Belgrader, Don Masquelier, Matthew Estes, Bruce Chabansky, Andrew Garst, Richard Fox, Jorge Bernate
  • INSTRUMENTS, MODULES, AND METHODS FOR IMPROVED DETECTION OF EDITED SEQUENCES IN LIVE CELLS
    Publication number: 20200347342
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Application
    Filed: July 15, 2020
    Publication date: November 5, 2020
    Inventors: Phillip Belgrader, Don Masquelier, Andrew Garst, Richard Fox
  • Automated nucleic acid assembly and introduction of nucleic acids into cells
    Patent number: 10822621
    Abstract: In an illustrative embodiment, automated instruments comprising one or more flow-through electroporation devices or modules are provided to automate transformation of nucleic acids in live cells.
    Type: Grant
    Filed: August 22, 2019
    Date of Patent: November 3, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier, Phillip Belgrader
  • AUTOMATED CELL PROCESSING INSTRUMENTS COMPRISING REAGENT CARTRIDGES
    Publication number: 20200338561
    Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment. The reagent cartridges may include an electroporation device, as well as sample receptacles, reagent receptacles, waste receptacles and the like, and a script for controlling a processor to dispense samples and reagents contained in the receptacles, and to porate cells in the electroporation device. Also described are kits including the cartridges, automated multi-module cell processing instruments including the reagent cartridges and methods of using the reagent cartridges.
    Type: Application
    Filed: July 14, 2020
    Publication date: October 29, 2020
    Inventors: Jorge Bernate, Don Masquelier, Phillip Belgrader, Brian Van Hatten, Bruce Chabansky
  • AUTOMATED CELL PROCESSING METHODS, MODULES, INSTRUMENTS, AND SYSTEMS
    Publication number: 20200332285
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Application
    Filed: July 6, 2020
    Publication date: October 22, 2020
    Inventors: Phillip Belgrader, Don Masquelier, Jorge Bernate, Ryan Gill, Kevin Ness
  • Automated cell processing instruments comprising reagent cartridges
    Patent number: 10799868
    Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment. The reagent cartridges may include an electroporation device, as well as sample receptacles, reagent receptacles, waste receptacles and the like, and a script for controlling a processor to dispense samples and reagents contained in the receptacles, and to porate cells in the electroporation device. Also described are kits including the cartridges, automated multi-module cell processing instruments including the reagent cartridges and methods of using the reagent cartridges.
    Type: Grant
    Filed: July 14, 2020
    Date of Patent: October 13, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier, Phillip Belgrader, Brian Van Hatten, Bruce Chabansky
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10801008
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: July 15, 2020
    Date of Patent: October 13, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Andrew Garst, Richard Fox
  • Electroporation cuvettes for automation
    Patent number: 10787683
    Abstract: The present disclosure relates to an electroporation device that may include many electroporation units and electroporation systems that can be used in an automated environment, e.g., as one station or module in a multi-station or multi-module cell processing environment.
    Type: Grant
    Filed: July 13, 2020
    Date of Patent: September 29, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Phil Belgrader, Don Masquelier, Vlorent Morina
  • Automated cell processing methods, modules, instruments, and systems
    Patent number: 10787663
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: July 6, 2020
    Date of Patent: September 29, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Jorge Bernate, Ryan Gill, Kevin Ness
  • Instruments, modules, and methods for improved detection of edited sequences in live cells
    Patent number: 10760043
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: March 18, 2020
    Date of Patent: September 1, 2020
    Assignee: Inscripta, Inc.
    Inventors: Andrew Garst, Richard Fox, Phillip Belgrader, Don Masquelier
  • AUTOMATED CELL PROCESSING INSTRUMENTS COMPRISING REAGENT CARTRIDGES
    Publication number: 20200269250
    Abstract: The present disclosure provides a reagent cartridge configured for use in an automated multi-module cell processing environment.
    Type: Application
    Filed: March 23, 2020
    Publication date: August 27, 2020
    Inventors: Brian Van Hatten, Don Masquelier, Phillip Belgrader, Jorge Bernate, Bruce Chabansky