Patents by Inventor Donald J. Merlo
Donald J. Merlo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20200385751Abstract: The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Streptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins. DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.Type: ApplicationFiled: August 20, 2020Publication date: December 10, 2020Applicant: Dow AgroSciences LLCInventors: Justin M Lira, Terry Wright, Andrew E. Robinson, Sean Russell, Donald J Merlo, Steven Webb, Nicole L Arnold, Kelley E Smith
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Publication number: 20200385752Abstract: The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Streptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins. DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes areas selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.Type: ApplicationFiled: August 20, 2020Publication date: December 10, 2020Applicant: Dow AgroSciences LLCInventors: Justin M Lira, Terry Wright, Andrew E. Robinson, Sean Russell, Donald J Merlo, Steven Webb, Nicole L Arnold, Kelley E Smith
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Patent number: 10793612Abstract: This disclosure concerns methods for the design of synthetic nucleic acid sequences that encode polypeptide amino acid repeat regions. This disclosure also concerns the use of such sequences to express a polypeptide of interest that comprises amino acid repeat regions, and organisms comprising such sequences.Type: GrantFiled: July 16, 2013Date of Patent: October 6, 2020Assignee: Dow AgroSciences LLCInventors: Donald J. Merlo, Ignacio Larrinua, Scott Bevan
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Patent number: 10752913Abstract: The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.Type: GrantFiled: December 7, 2007Date of Patent: August 25, 2020Assignee: Dow AgroSciences LLCInventors: Justin M. Lira, Terry R. Wright, Sean M. Russell, Donald J. Merlo, Steven R. Webb, Nicole L. Arnold, Andrew E. Robinson, Kelley A. Smith
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Patent number: 10450549Abstract: The present disclosure relates to the use of a grass-active herbicide postemergently applied to AAD1-transformed turfgrasses to selectively control grass weeds in a turf grass crop. Also described is the use of AAD1 as a selectable marker in the production of transgenic turfgrass.Type: GrantFiled: October 6, 2016Date of Patent: October 22, 2019Assignee: Dow AgroSciences LLCInventors: Carla N. Yerkes, Barbara A. Zilinskas, Donald J. Merlo, Terry R. Wright, Justin Lira, Nicole Arnold, Gary D. Thompson, Ning Zhou, Andrew Worden
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Publication number: 20180346926Abstract: The invention provides synthetic nucleic acid sequences encoding proteins of interest that are particularly adapted to express well in plants. The claimed synthetic sequences utilize plant-optimized codons roughly in the same frequency at which they are utilized, on average, in genes naturally occurring in the plant species. The invention further includes synthetic DNA sequence for herbicide tolerance, water and/or heat stress tolerance, healthy oil modifications and for transformation marker genes and selectable marker genes are used. DNA construct and transgenic plants containing the synthetic sequences are taught as are methods and compositions for using the plants in agriculture.Type: ApplicationFiled: July 30, 2018Publication date: December 6, 2018Inventors: Ignacio Mario Larrinua, Donald J. Merlo, Avutu S. Reddy, Arvind Kumar ThirumalaiswamySekhar, Aaron T. Woosley
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Publication number: 20170183673Abstract: Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains.Type: ApplicationFiled: March 8, 2017Publication date: June 29, 2017Inventors: Donald J. Merlo, Sean M. Russell, Diane Retallack, Aaron Woosley, Tom Meade, Kenneth E. Narva
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Patent number: 9617551Abstract: Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains.Type: GrantFiled: July 29, 2011Date of Patent: April 11, 2017Assignee: Dow AgroSciences LLCInventors: Donald J. Merlo, Sean M. Russell, Diane M. Retallack, Aaron T. Woosley, Thomas Meade, Kenneth Narva
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Publication number: 20170088855Abstract: The present disclosure relates to the use of a grass-active herbicide postemergently applied to AAD1-transformed turfgrasses to selectively control grass weeds in a turf grass crop. Also described is the use of AAD1 as a selectable marker in the production of transgenic turfgrass.Type: ApplicationFiled: October 6, 2016Publication date: March 30, 2017Inventors: Carla N. Yerkes, Barbara A. Zilinskas, Donald J. Merlo, Terry R. Wright, Justin Lira, Nicole Arnold, Gary D. Thompson, Ning Zhou, Andrew Worden
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Patent number: 9434773Abstract: This invention relates generally to a plant cell with increased yield, preferably under condition of transient and repetitive abiotic stress as compared to a corresponding non-transformed wild type plant cell by increasing or generating one or more activities of RING proteins in plants.Type: GrantFiled: September 14, 2012Date of Patent: September 6, 2016Assignee: Dow AgroSciences LLCInventors: Avutu S. Reddy, Sun Yuejin, Donald J. Merlo, Weiting W. Ni, Dayakar Pareddy, Zhifang Gao, Gregory A. Bradfisch
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Patent number: 9427003Abstract: The invention provides synthetic nucleic acid sequences encoding proteins of interest that are particularly adapted to express well in plants. The claimed synthetic sequences utilize plant-optimized codons roughly in the same frequency at which they are utilized, on average, in genes naturally occurring in the plant species. The invention further includes synthetic DNA sequence for herbicide tolerance, water and/or heat stress tolerance, healthy oil modifications and for transformation marker genes and selectable marker genes are used. DNA construct and transgenic plants containing the synthetic sequences are taught as are methods and compositions for using the plants in agriculture.Type: GrantFiled: April 16, 2012Date of Patent: August 30, 2016Assignee: Dow AgroSciences LLCInventors: Ignacio M. Larrinua, Donald J. Merlo, Avutu S. Reddy, Arvind Kumar ThirumalaiswamySekhar, Aaron T. Woosley
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Publication number: 20150267213Abstract: Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains.Type: ApplicationFiled: July 29, 2011Publication date: September 24, 2015Applicant: DOW AGROSCIENCES LLCInventors: Donald J. Merlo, Sean M. Russell, Diane M. Retallack, Aaron T. Woosley, Thomas Meade, Kenneth Narva
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Patent number: 9127289Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No ?-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action.Type: GrantFiled: November 22, 2010Date of Patent: September 8, 2015Assignee: Dow AgroSciences LLCInventors: Terry R. Wright, Justin M. Lira, Donald J. Merlo, Nicole L. Arnold
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Publication number: 20150175672Abstract: This disclosure concerns methods for the design of synthetic nucleic acid sequences that encode polypeptide amino acid repeat regions. This disclosure also concerns the use of such sequences to express a polypeptide of interest that comprises amino acid repeat regions, and organisms comprising such sequences.Type: ApplicationFiled: July 16, 2013Publication date: June 25, 2015Inventors: Donald J. Merlo, Ignacio Larrinua, Scott Bevan
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Publication number: 20130067617Abstract: This invention relates generally to a plant cell with increased yield, preferably under condition of transient and repetitive abiotic stress as compared to a corresponding non-transformed wild type plant cell by increasing or generating one or more activities of RING proteins in plants.Type: ApplicationFiled: September 14, 2012Publication date: March 14, 2013Inventors: Avutu S. Reddy, Sun Yuejin, Donald J. Merlo, Weiting W. Ni, Dayakar Pareddy, Zhifang Gao, Gregory A. Bradfisch
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Publication number: 20120266335Abstract: The invention provides synthetic nucleic acid sequences encoding proteins of interest that are particularly adapted to express well in plants. The claimed synthetic sequences utilize plant-optimized codons roughly in the same frequency at which they are utilized, on average, in genes naturally occurring in the plant species. The invention further includes synthetic DNA sequence for herbicide tolerance, water and/or heat stress tolerance, healthy oil modifications and for transformation marker genes and selectable marker genes are used. DNA construct and transgenic plants containing the synthetic sequences are taught as are methods and compositions for using the plants in agriculture.Type: ApplicationFiled: April 16, 2012Publication date: October 18, 2012Applicant: DOW AGROSCIENCES LLCInventors: IGNACIO M. LARRINUA, DONALD J. MERLO, AVUTU S. REDDY, ARVIND KUMAR THIRUMALAISWAMYSEKHAR, AARON T. WOOSLEY
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Patent number: 8283522Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.Type: GrantFiled: October 27, 2006Date of Patent: October 9, 2012Assignee: Dow AgroSciences, LLC.Inventors: Terry R. Wright, Justin M. Lira, Terence Anthony Walsh, Donald J. Merlo, Jayakumar P. Samuel, Gaofeng Lin
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Patent number: 8278505Abstract: The subject invention provides novel plants that are not only resistant to 2,4-D, but also to a pyridyloxyacetate herbicide. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-13 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.Type: GrantFiled: May 9, 2008Date of Patent: October 2, 2012Assignee: Dow AgroSciences, LLC.Inventors: Justin M. Lira, Erika Megan Snodderley, Andrew E. Robinson, Terry R. Wright, Donald J. Merlo
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Patent number: 8084418Abstract: The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibacillus, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a “stand-alone” TC protein (from Photorhabdus, Xenorhabdus, or Paenibacillus, for example) is enhanced by one or more TC protein “potentiators” derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand-alone Photorhabdus protein toxin.Type: GrantFiled: February 16, 2009Date of Patent: December 27, 2011Assignee: Dow AgroSciences LLCInventors: Timothy D. Hey, Amanda D. Schleper, Scott A. Bevan, Scott B. Bintrim, Jon C. Michell, Ze Sheng Li, Weiting Ni, Baolong Zhu, Donald J. Merlo, Patricia C. Apel-Birkhold, Thomas Meade
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Patent number: 8003777Abstract: The present invention provides methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 5? and 3? untranslated regions of genes encoding osmotin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional procedures, resulting in enhanced expression of the selected coding region. In a preferred embodiment, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.Type: GrantFiled: February 5, 2008Date of Patent: August 23, 2011Assignee: Dow AgroSciences LLCInventors: Donald J. Merlo, Dong Liu, Stephanie L. Burton, Todd P. Glancy