Abstract: The present invention relates to a method of manufacturing collagen for restoring cartilage tissue usable for a joint in an injection manner, and a usage method thereof. The present invention provides a method of obtaining a high-concentration collagen-solution injection by aseptically filling an injection container with collagen separated from pig skin tissue, and a method of using the high-viscosity collagen loaded into the injection container for the purpose of cartilage restoration. Cartilage tissue regeneration is effectively induced when tissue restoration is implemented with respect to a cartilage-deficient portion using collagen, which is a biocompatible material, in a form that is capable of being injected into an application site with an injection needle without a surgical incision. Accordingly, restoration and regeneration of cartilage are easily and quickly induced in an animal, excluding a human, while relieving the burden related to surgery.

Abstract: Disclosed is a medical material manufactured using collagen and a method of manufacturing the same. The method includes (1) preparing collagen using distilled water as a solvent, (2) filling a syringe with the prepared collagen and then spinning the collagen through a syringe needle, (3) immediately immersing the spun collagen in a cross-linking solution, which is a mixture including therein a hyperosmotic agent and a cross-linking agent mixed with each other, (4) removing and then washing the collagen after cross-linking is completed, and (5) removing and then drying the collagen after the washing is completed. When the collagen is spun and processed into the form of a thread and the spun thread is then cross-linked, the cross-linked collagen thread has increased strength compared to before cross-linking, and the shape thereof is retained in an aqueous solution.

Abstract: Disclosed is a method of producing high-concentration collagen for use as a medical material, including: washing tissue of a mammal; subjecting the washed tissue to crushing and immersion in ethyl alcohol; subjecting the tissue to enzymatic treatment with stirring in purified water containing phosphoric acid and pepsin; adding sodium chloride to the collagen subjected to enzymatic treatment, performing stirring, and aggregating collagen; dissolving the aggregated collagen in purified water to give a collagen solution, which is then filtered using a filter and concentrated by removing the pepsin, low-molecular-weight material, and sodium chloride from the collagen solution using a tangential flow filtration device; subjecting the concentrated collagen to sterile filtration, aggregating the collagen using a pH solution in a neutralization tank, and concentrating the collagen by removing a non-aggregated solution; and concentrating the concentrated collagen using a centrifuge and stirring the concentrated collag

Abstract: Disclosed are a matrix for restoring soft tissue and a method of producing the same, the method including: forming a sheet-type absorption layer (11) on one side of a double layer using biocompatible neutral collagen; forming a film-type support layer (12) on the remaining surface of the double layer by mixing collagen with a water-soluble polymer and a natural material; and forming a double layer-structured matrix (10) for restoring soft tissue by attaching the absorption layer (11) and the support layer (12) so as to treat damage to soft tissue of a mammal other than a human or restore damaged soft tissue. This invention pertains to the treatment of damage to soft tissue such as tendons, ligaments, and rotator cuffs or the restoration of damaged soft tissue, whereby the quality and reliability of products can be remarkably improved, thus satisfying various needs of consumers, who are the users thereof, and exhibiting a good effect.

Abstract: Disclosed is a medical material manufactured using collagen and a method of manufacturing the same. The method includes (1) preparing collagen using distilled water as a solvent, (2) filling a syringe with the prepared collagen and then spinning the collagen through a syringe needle, (3) immediately immersing the spun collagen in a cross-linking solution, which is a mixture including therein a hyperosmotic agent and a cross-linking agent mixed with each other, (4) removing and then washing the collagen after cross-linking is completed, and (5) removing and then drying the collagen after the washing is completed. When the collagen is spun and processed into the form of a thread and the spun thread is then cross-linked, the cross-linked collagen thread has increased strength compared to before cross-linking, and the shape thereof is retained in an aqueous solution.

Abstract: The present invention relates to a method of manufacturing collagen for restoring cartilage tissue usable for a joint in an injection manner, and a usage method thereof. The present invention provides a method of obtaining a high-concentration collagen-solution injection by aseptically filling an injection container with collagen separated from pig skin tissue, and a method of using the high-viscosity collagen loaded into the injection container for the purpose of cartilage restoration. Cartilage tissue regeneration is effectively induced when tissue restoration is implemented with respect to a cartilage-deficient portion using collagen, which is a biocompatible material, in a form that is capable of being injected into an application site with an injection needle without a surgical incision. Accordingly, restoration and regeneration of cartilage are easily and quickly induced in an animal, excluding a human, while relieving the burden related to surgery.

Abstract: Disclosed is a basic culture medium for mesenchymal stem cells, and a cell therapeutic agent cultured and differentiated using same. The basic culture medium reduces the time taken from collection to mass culturing by increasing the proliferation rate of undifferentiated mesenchymal stem cells derived from an adult tissue such as human marrow and adipose tissue, and also is capable of various differentiations into treating agents for bone-forming cells, for cartilage cells, or for fat cells.

Abstract: Disclosed is a method of producing high-concentration collagen for use as a medical material, including: washing tissue of a mammal; subjecting the washed tissue to crushing and immersion in ethyl alcohol; subjecting the tissue to enzymatic treatment with stirring in purified water containing phosphoric acid and pepsin; adding sodium chloride to the collagen subjected to enzymatic treatment, performing stirring, and aggregating collagen; dissolving the aggregated collagen in purified water to give a collagen solution, which is then filtered using a filter and concentrated by removing the pepsin, low-molecular-weight material, and sodium chloride from the collagen solution using a tangential flow filtration device; subjecting the concentrated collagen to sterile filtration, aggregating the collagen using a pH solution in a neutralization tank, and concentrating the collagen by removing a non-aggregated solution; and concentrating the concentrated collagen using a centrifuge and stirring the concentrated collag

Abstract: Disclosed are a matrix for restoring soft tissue and a method of producing the same, the method including: forming a sheet-type absorption layer (11) on one side of a double layer using biocompatible neutral collagen; forming a film-type support layer (12) on the remaining surface of the double layer by mixing collagen with a water-soluble polymer and a natural material; and forming a double layer-structured matrix (10) for restoring soft tissue by attaching the absorption layer (11) and the support layer (12) so as to treat damage to soft tissue of a mammal other than a human or restore damaged soft tissue. This invention pertains to the treatment of damage to soft tissue such as tendons, ligaments, and rotator cuffs or the restoration of damaged soft tissue, whereby the quality and reliability of products can be remarkably improved, thus satisfying various needs of consumers, who are the users thereof, and exhibiting a good effect.

Abstract: Disclosed are a biomaterial imparted with enhanced rubber properties through natural cross-linking between collagen and hyaluronic acid, a method of preparing the same, and a method of using the same, wherein the rubber-type biomaterial is prepared from collagen and hyaluronic acid at a ratio of 2:1 to 7:1 through natural cross-linking. Specifically, a novel material that exhibits rubber properties can be prepared from collagen and hyaluronic acid at an optimal mixing ratio taking into consideration the molecular structural properties thereof under natural cross-linking conditions, rather than chemical or physical cross-linkage, thereby remarkably improving the quality and reliability of products, ultimately fulfilling various needs of consumers to thus increase marketability.

Abstract: The present invention relates to a tissue sealant in which collagen and fibrin are mixed and to a method for preparing same. To this end, the method of the present invention comprises the steps of: mixing a first material using fibrinogen and aprotinin; mixing a second material using thrombin, calcium chloride, and collagen; and preparing a third material by mixing the first material and the second material. The tissue sealant prepared by the method may supplement weaknesses, i.e. strength and degradability, of the fibrin sealant which is currently available in the commercial marketplace. Further, the tissue sealant of the present invention is cytophilic and activates blood platelets contained in blood so as to induce tissue regeneration. Thus, quality and reliability of products can be significantly improved so as to satisfy various needs of consumers who are users, thereby presenting good image.

Abstract: The present invention relates to a composition for repairing cartilage tissue, to a method for producing same, and to the use thereof. The present invention provides a composition for repairing cartilage tissue, which is obtained by mixing collagen and hyaluronic acid using a two-way syringe or a mixer, wherein the collagen has a diluted concentration of 5-60 mg/mL excepting water or a physiological phosphate buffer solution, and the hyaluronic acid has a diluted concentration of 5-20 mg/mL excepting water or a physiological phosphate buffer solution.

Abstract: Disclosed is a basic culture medium for mesenchymal stem cells, and a cell therapeutic agent cultured and differentiated using same. The basic culture medium reduces the time taken from collection to mass culturing by increasing the proliferation rate of undifferentiated mesenchymal stem cells derived from an adult tissue such as human marrow and adipose tissue, and also is capable of various differentiations into treating agents for bone-forming cells, for cartilage cells, or for fat cells.

Abstract: Cell manipulation and cultivation equipment for the production of cell therapy products has developed for preventing contamination of cells and improving the utility of a space. The equipment includes a room for the production and testing of cell therapy products, the room having an inner space enclosed in all sides and an outer entrance/exit door provided at any one side thereof, an L-shaped partition installed in a position of the room for the production and testing of cell therapy products, the partition having an inner entrance/exit door provided at any one side thereof, and a clean bench device placed inside the partition, the clean bench device including first, second, and third clean benches for preventing contamination of cells and increasing the utility of a space to the maximum extent. The operating of the clean room and clean bench has advantages to prevent contamination and improving the quality and reliability of cell therapy products.

Abstract: A facility module is provided for producing and storing a cell therapy product comprising: a Cell Therapy (CT) module one including separately prefabricated units having specific functions and separate entrances and exits so as to minimize contamination, and being capable of producing the cell therapy product, and a Banking of Cell and Tissue (BC) module Two including prefabricated units having specific functions and separate entrances and exits so as to minimize contamination, and being capable of appropriately storing hematopoietic stem cells, bone marrow cells and other cells for a prolonged period. It enables easy and low cost production of the cell therapy product, with sufficient quality to be transplanted into patients, within a short period, and permits clinical application to patients expeditiously.

Abstract: A method is provided for utilizing a cell therapy product facility and an internet franchise market business comprising: producing a cell therapy product by a CT (Cell Therapy)-module (1) of prefabricated units having specific functions, and; storing of hematopoietic stem cells, bone marrow cells and other cells, by a BC (Banking of Cell and Tissue)-module (2) of prefabricated units having specific functions. Both modules eliminate contamination and maximize efficiency. It provides an operating method and a use method and business method for the above cell therapy product facility. The invention enables easy, low cost production of a product having sufficient quality to be transplanted into patients and put into clinical application within a short period of time. The facility module of the invention can be installed and used wherever adequate space is available. The invention provides improved quality and reliability of cell therapy products.