Abstract: A control system for positioning a marker over a pre-existing roadway surface mark. The control system has one or more imagers having a field of view for imaging an area of roadway mark surface encompassing the roadway mark and a computer having a machine learning network to process the roadway mark image and position the marker over the pre-existing roadway mark.

Abstract: A map control module receives a stream of map data characterizing a geographic region proximal to a vehicle and outputs a moving map, and the moving map is divisible into a matrix of cells. A map item control receives a stream of point of interest (POI) data characterizing a plurality of POIs within the boundary and categorizes each of the plurality of POIs to define a set of categories. The map item control determines a display location of a map item within the matrix of cells for each of the plurality of POIs and determines an importance for each POI within a same category. The map item control forms a set of clusters of map items. Each map item in a given cluster of map items has a same cell and a same category. The map item control selects a top-item for each cluster of map items.

Abstract: A heating, ventilating, and air conditioning (HVAC) system includes a refrigerant loop having a compressor, where the compressor is configured to circulate a refrigerant through the refrigerant loop, a first heat exchanger disposed along the refrigerant loop, where the first heat exchanger is configured to place the refrigerant in a first heat exchange relationship with a working fluid, and an air handling unit having a second heat exchanger, where the second heat exchanger is configured to place the working fluid in a second heat exchange relationship with an airflow, and where the air handling unit is isolated from the first heat exchanger to reduce or eliminate mixing of refrigerant with the airflow.

Abstract: A method of treatment of a subject suffering from autonomic dysfunction due to opioid use is described. The autonomic dysfunction can be determined by, for example, detecting genetic damage, such as DNA hypermethylation, measuring catecholamine concentrations, collecting vitals data, and/or surveys. The method of treatment can include administering a partial opioid agonist, such as buprenorphine.

Abstract: Methods for producing a paired tag from a nucleic acid sequence are provided in which the paired tag comprises the 5? end tag and 3? end tag of the nucleic acid sequence. In one embodiment, the nucleic acid sequence comprises two restriction endonuclease recognition sites specific for a restriction endonuclease that cleaves the nucleic acid sequence distally to the restriction endonuclease recognition sites. In another embodiment, the nucleic acid sequence further comprises restriction endonuclease recognition sites specific for a rare cutting restriction endonuclease. Methods of using paired tags are also provided. In one embodiment, paired tags are used to characterize a nucleic acid sequence. In a particular embodiment, the nucleic acid sequence is a genome. In one embodiment, the characterization of a nucleic acid sequence is karyotyping. Alternatively, in another embodiment, the characterization of a nucleic acid sequence is mapping of the sequence.

Abstract: The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.

Abstract: The present invention provides microarrays of oligonucleotide primer pairs, and in particular, microarrays of primers that comprise at least one cleavable linkage. Also provided are methods to capture oligonucleotide primer pairs from one or more microarrays, and methods to use the captured oligonucleotide primer pairs, such as for amplification of a target polynucleotide sequence. In addition, methods of using a microarray to isolate, purify and/or amplify a target polynucleotide are provided.

Abstract: The present invention provides microarrays of oligonucleotide primer pairs, and in particular, microarrays of primers that comprise at least one cleavable linkage. Also provided are methods to capture oligonucleotide primer pairs from one or more microarrays, and methods to use the captured oligonucleotide primer pairs, such as for amplification of a target polynucleotide sequence. In addition, methods of using a microarray to isolate, purify and/or amplify a target polynucleotide are provided.

Abstract: A three-way hitch assembly for a tractor-type vehicle, such as an ATV/UTV, is designed to facilitate the attachment of multiple accessory devices to the vehicle. The hitch assembly includes: a drawbar pintle, a hook, and a replaceable hitch ball.

Abstract: The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.

Abstract: The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.

Abstract: The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.

Abstract: A pair of asymmetrical, partially double-stranded oligonucleotide adapters are provided wherein the pair of adapters comprise a first asymmetrical oligonucleotide adapter comprising a single-stranded 3? overhang and a second asymmetrical double-stranded oligonucleotide adapter comprising a single-stranded 5? overhang and at least one blocking group on the strand of said second asymmetrical oligonucleotide adapter that does not comprise the 5? overhang. Also provided are a pair of double-stranded Y oligonucleotide adapters and a pair of double-stranded bubble oligonucleotide adapters and methods of using said asymmetrical adapters for amplification of at least one double stranded nucleic acid molecule, wherein the amplification produces a plurality of amplified nucleic acid molecules having a different nucleic acid sequence at each end are also described. Also provided is a method for exponentially amplifying one strand in a double-stranded nucleic acid molecule.

Abstract: A method for fabricating a nozzle includes providing at least two plates. At least one of the at least two plates includes a first opening. Each plate includes at least one mating surface. The method also includes stacking the at least two plates together such that a mating surface of a first of the at least two plates is substantially flush against a mating surface of a second of the at least two plates, and such that a fluid passage is at least partially defined by the at least two plates. The method also includes orienting the at least two plates relative to each other such that the opening within at least one of the at least two plates at least partially defines an outlet for discharging fluid from the fluid passage.

Abstract: Methods for producing a paired tag from a nucleic acid sequence are provided in which the paired tag comprises the 5? end tag and 3? end tag of the nucleic acid sequence. In one embodiment, the nucleic acid sequence comprises two restriction endonuclease recognition sites specific for a restriction endonuclease that cleaves the nucleic acid sequence distally to the restriction endonuclease recognition sites. In another embodiment, the nucleic acid sequence further comprises restriction endonuclease recognition sites specific for a rare cutting restriction endonuclease. Methods of using paired tags are also provided. In one embodiment, paired tags are used to characterize a nucleic acid sequence. In a particular embodiment, the nucleic acid sequence is a genome. In one embodiment, the characterization of a nucleic acid sequence is karyotyping. Alternatively, in another embodiment, the characterization of a nucleic acid sequence is mapping of the sequence.

Abstract: The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.

Abstract: The present invention provides microarrays of oligonucleotide primer pairs, and in particular, microarrays of primers that comprise at least one cleavable linkage. Also provided are methods to capture oligonucleotide primer pairs from one or more microarrays, and methods to use the captured oligonucleotide primer pairs, such as for amplification of a target polynucleotide sequence. In addition, methods of using a microarray to isolate, purify and/or amplify a target polynucleotide are provided.

Abstract: A manually operated lifting device for adaptably lifting the front or back end of a lawn mower, garden tractor or similar small, powered vehicle, or to lift these vehicles from the side, is provided. The lifting device comprises a vertical frame having removable front arms at the bottom end and extending forward for engaging the axle of the mower or tractor, either at the front or back. The lifting device also comprises a horizontal arm at the bottom of the frame, for positioning and securing the vehicle while being lifted from the side, a collapsible base member and a threaded rod connect to the base member and the frame, such that turning the rod raises the frame from the base, thus lifting the vehicle. The rod may be turned using a hand crank or an electrical device such as an electric hand drill.

Abstract: A three-way hitch assembly for a tractor-type vehicle, such as an ATV/UTV, is designed facilitates the attachment of multiple accessory devices to the vehicle. The hitch assembly includes: (a) a receiver portion adapted to be attached to a vehicle, said receiver portion having a profiled opening for receiving a drawbar portion; (b) a drawbar portion having a matching profile to said receiver portion and adapted to be inserted in the receiver portion in one of a plurality of orientations as required for a particular application; (c) means for retaining the drawbar portion in the receiver portion in said one orientation; (d) a hook attached to one side of the drawbar portion adapted to receive a tow chain or strap; (e) a pintle attached to one side of the drawbar portion adapted to receive a pin/pintle attachment accessory; and (f) a trailer ball attached to one side of the drawbar portion adapted to receive a trailer hitch mount.

Abstract: The present teachings relate to improved methods, kits, and compositions for making nucleic acid libraries and sequencing nucleic acids. In some embodiments, directionally defined concatamers are generated, facilitating sequencing efforts.