Patents by Inventor Duanqing Pei
Duanqing Pei has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20210047624Abstract: Disclosed herein is a culture system for chemical induction of pluripotent stem cells, comprising a basic culture medium and a composition for performing chemical induction of reprogramming process. The said composition comprises a thymine analogue, a cAMP activator, a TGF-? receptor inhibitor, a bone morphogenetic protein, a RA receptor activator, a GSK3 inhibitor and a basic fibroblast growth factor. And the said culture system is free of serum. By using the culture system as described herein, there is no need to frequently replate cells during culture, such that culturing process is simplified and loss of cells resulting from replating cells is reduced. As the culture system is free of serum, subsequent collection of pluripotent stem cells and molecular mechanism analysis are simplified, thereby facilitating establishment of no animal origin culture systems for induction of pluripotent stem cells.Type: ApplicationFiled: February 28, 2019Publication date: February 18, 2021Inventors: Duanqing PEI, Shangtao CAO, Jing LIU, Shengyong YU, Jiekai CHEN, Jing YE, Dongwei LI
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Publication number: 20180237745Abstract: Provided is a method for controlling differentiation of pluripotent stem cells into endoderm-derived cells, and particularly for preventing, by using a TGF inhibitor or an SNAI1 inhibitor, differentiation of pluripotent stem cells into the endoderm-derived cells.Type: ApplicationFiled: August 10, 2016Publication date: August 23, 2018Inventors: Duanqing Pei, Xiaodong Shu, Qiuhong Li, Andrew Hutchins
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Patent number: 10011819Abstract: Provided are a culture medium for preparing neural stem cell and use thereof, the culture medium for preparing neural stem cell comprising: a basic culture medium suitable for the growth of stem cell, and a cell signal pathway inhibitor selected from at least one of GSK inhibitor, MEK inhibitor, TGF-? inhibitor, ROCK inhibitor and BMP inhibitor.Type: GrantFiled: February 6, 2013Date of Patent: July 3, 2018Assignee: GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH, CHINESE ACADEMY OF SCIENCESInventors: Guangjin Pan, Duanqing Pei, Lihui Wang, Linli Wang, Yanting Xue
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Patent number: 9926532Abstract: Methods for generating iPSCs and differentiated cells of interest by reprogramming donor cells that have been obtained in a non-invasive manner. In particular, the donor cells are exfoliated epithelial urine cells. The differentiated cells can be obtained by differentiation of the reprogrammed iPSCs or by direct reprogramming the urine cells.Type: GrantFiled: December 23, 2011Date of Patent: March 27, 2018Assignees: Guangzhou Institute of Biomedicine and Health, Universitat fur Bodenkultur WienInventors: Miguel Esteban, Johannes Grillari, Regina Grillari, Duanqing Pei, Ting Zhou
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Patent number: 9782443Abstract: The usage of a stem cell in preparation of a tooth-like structure is provided. And a culture medium, a method for preparing an epithelial-like cell, a kit for preparing an ameloblast, a method for preparing an ameloblast are also provided. Specifically, the culture medium comprises a basal medium, which is DMEM/F12 medium; N2 supplement; retinoic acid; and BMP-4.Type: GrantFiled: February 5, 2013Date of Patent: October 10, 2017Assignee: Guangzhou Institutes Of Biomedicine And Health, Chinese Academy Of SciencesInventors: Duanqing Pei, Jinglei Cai, Pengfei Liu, Shubin Chen, Yanmei Zhang
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Publication number: 20170159017Abstract: The present disclosure provides a method for producing a cell with exogenous mitochondria by obtaining synthetic mitochondria via introduction of exogenous mitochondrial DNA into mitochondria or empty mitochondrial shells, and incorporating the same into mammalian cells via endocytosis. As such, effective functionality of exogenous mitochondria in cells is realized. The synthetic mitochondrial DNA genes introduced according to the present disclosure can be stably expressed and effectively passaged. The method for introducing exogenous mitochondrial DNA into mammalian cells as disclosed herein may be used as a whole new mitochondrial molecular cloning means to perform site-directed mutagenesis, gene insertion, gene knockout, gene rearrangement, and the like in mitochondria. Therefore, any molecular cloning modification can be performed on a mammalian mitochondrial DNA, which is of great importance to therapeutic schemes of diseases derived from mitochondrial DNA mutations.Type: ApplicationFiled: August 25, 2014Publication date: June 8, 2017Applicant: Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesInventors: Xingguo Liu, Duanqing Pei, Jinglei Liu, Deyang Yao, Xuebin Liu
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Publication number: 20170037376Abstract: Provided are a method for preparing an induced pluripotent stem cell and a composition used in the method. The method comprises: introducing a composition for promoting the formation of an induced pluripotent stem cell into a somatic cell, the composition comprising: (i) a c-Jun antagonist and one group of factors from among the following seven such groups: (1) Sox2, Klf4 and c-Myc, (2) Klf4 and c-Myc, (3) Oct3/4, Klf4 and c-Myc, (4) Sox2, Nanog and Lin28, (5) Oct3/4, Nanog and Lin28, (6) Oct3/4, Klf and Sox2, and (7) Klf4 and Sox2; or (ii) the c-Jun antagonist, Jhdm1b and Id1, and at least one of Glis1, Sall4 or Lrh1; or (iii) the c-Jun antagonist, Jhdm1b and Id1, and at least one of: Oct4, Klf4, Sox2, Lin28, Esrrb, Lef1, Utf1 or miRNA C. The present method allows for successful preparation of induced pluripotent stem cells with no generation of abnormal chromosomes.Type: ApplicationFiled: November 12, 2014Publication date: February 9, 2017Applicant: GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH, CHINESE ACADEMY OF SCIENCESInventors: Duanqing PEI, Jing LIU, Jiekai CHEN, Meixiu PENG
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Publication number: 20160000836Abstract: The usage of a stem cell in preparation of a tooth-like structure is provided. And a culture medium, a method for preparing an epithelial-like cell, a kit for preparing an ameloblast, a method for preparing an ameloblast are also provided. Specifically, the culture medium comprises a basal medium, which is DMEM/F 12 medium; N2 supplement; retinoic acid; and BMP-4.Type: ApplicationFiled: February 5, 2013Publication date: January 7, 2016Inventors: Duanqing PEI, Jinglei CAI, Pengfei LIU, Shubin CHEN, Yanmei ZHANG
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Patent number: 9163217Abstract: The present invention relates to a method for increasing the efficiency of inducing pluripotent stem cells by utilizing genes Jhdm1b and Jhdm1a that modify histone. By utilizing Jhdm1b, Jhdm1a, and a stem cell inducing factor, the present invention increases the efficiency of inducing pluripotent stem cells and increases the quality of induced pluripotent stem cells. The stem cell inducing factor is a combination of Oct4 and Klf4, or a combination of Sox2, Oct4, and Klf4, or a combination of Oct4 and Sox2, and Oct4 alone. The method further comprises exposing the cells to vitamin C, which further increases the efficiency of inducing pluripotent stem cells as compared with the case where no vitamin C is used. By using less stem cell reducing factors, the method of the present invention reduces the potential carcinogenicity, obtains a high inducing efficiency, and provides high-quality induced pluripotent stem cells capable of germ-line transmission.Type: GrantFiled: March 31, 2012Date of Patent: October 20, 2015Assignee: GUANGZHOU INSTITTUTES OF BIOMEDICINE AND HEALTH, CHINESE ACADEMY OF SCIENCESInventors: Duanqing Pei, Tao Wang
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Patent number: 9163218Abstract: The present invention relates to a method for increasing the efficiency of inducing pluripotent stem cells by utilizing genes Jhdm1a that modify histone. By utilizing Jhdm1a, and a stem cell inducing factor, the present invention increases the efficiency of inducing pluripotent stem cells and increases the quality of induced pluripotent stem cells. The stem cell inducing factor is a combination of Oct4 and Klf4, or a combination of Sox2, Oct4, and Klf4, or a combination of Oct4 and Sox2, and Oct4 alone. The method further comprises exposing the cells to vitamin C, which further increases the efficiency of inducing pluripotent stem cells as compared with the case where no vitamin C is used. By using less stem cell reducing factors, the method of the present invention reduces the potential carcinogenicity, obtains a high inducing efficiency, and provides high-quality induced pluripotent stem cells capable of germ-line transmission.Type: GrantFiled: August 27, 2013Date of Patent: October 20, 2015Assignee: GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH CHINESE ACADEMY OF SCIENCESInventors: Duanqing Pei, Tao Wang
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Publication number: 20150030570Abstract: Provided are a culture medium for preparing neural stem cell and use thereof, the culture medium for preparing neural stem cell comprising: a basic culture medium suitable for the growth of stem cell, and a cell signal pathway inhibitor selected from at least one of GSK inhibitor, MEK inhibitor, TGF-? inhibitor, ROCK inhibitor and BMP inhibitor.Type: ApplicationFiled: February 6, 2013Publication date: January 29, 2015Applicant: Guangzhou Institutes of Biomedicine and Health Chinese Academy of SciencesInventors: Guangjin Pan, Duanqing Pei, Lihui Wang, Linli Wang, Yanting Xue
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Patent number: 8846671Abstract: The heterocyclic alkynyl benzene compounds of formula (I), their pharmaceutically acceptable salts and stereoisomers thereof, as well as application in preparing drugs for preventing or treating tumors. The compounds can overcome the clinically induced resistance against Gleevec.Type: GrantFiled: June 3, 2011Date of Patent: September 30, 2014Assignee: Guangzhou Institute of Biomedicine and Health, Chinese Academy of SciencesInventors: Ke Ding, Deping Wang, Duanqing Pei, Zhang Zhang, Mengjie Shen, Kun Luo, Yubing Feng
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Publication number: 20140193912Abstract: The present invention relates to a method for increasing the efficiency of inducing pluripotent stem cells by utilizing genes Jhdm1a that modify histone. By utilizing Jhdm1a, and a stem cell inducing factor, the present invention increases the efficiency of inducing pluripotent stem cells and increases the quality of induced pluripotent stem cells. The stem cell inducing factor is a combination of Oct4 and Klf4, or a combination of Sox2, Oct4, and Klf4, or a combination of Oct4 and Sox2, and Oct4 alone. The method further comprises exposing the cells to vitamin C, which further increases the efficiency of inducing pluripotent stem cells as compared with the case where no vitamin C is used. By using less stem cell reducing factors, the method of the present invention reduces the potential carcinogenicity, obtains a high inducing efficiency, and provides high-quality induced pluripotent stem cells capable of germ-line transmission.Type: ApplicationFiled: August 27, 2013Publication date: July 10, 2014Applicant: GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH, CHINESE ACADEMY OF SCIENCESInventors: Duanqing PEI, Tao WANG
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Publication number: 20130323782Abstract: Methods for generating iPSCs and differentiated cells of interest by reprogramming donor cells that have been obtained in a non-invasive manner. In particular, the donor cells are exfoliated epithelial urine cells. The differentiated cells can be obtained by differentiation of the reprogrammed iPSCs or by direct reprogramming the urine cells.Type: ApplicationFiled: December 23, 2011Publication date: December 5, 2013Applicants: Guangzhou Institute of Biomedicine and Health, Universitat fur Bodenkultur WienInventors: Miguel Esteban, Johannes Grillari, Regina Grillari, Duanqing Pei, Ting Zhou
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Publication number: 20130302893Abstract: The present invention relates to a method for increasing the efficiency of inducing pluripotent stem cells, and more particularly, to a method for increasing the efficiency of inducing pluripotent stem cells by utilizing genes Jhdm1b and Jhdm1a that modify histone. By utilizing Jhdm1b, Jhdm1a, and a stem cell inducing factor, the present invention increases the efficiency of inducing pluripotent stem cells and increases the quality of induced pluripotent stem cells. The stem cell inducing factor is a combination of Oct4 and Klf4, or a combination of Sox2, Oct4, and Klf4, or a combination of Oct4 and Sox2, and Oct4 alone. The method of the present invention further comprises exposing the cells to vitamin C, which further increases the efficiency of inducing pluripotent stem cells as compared with the case where no vitamin C is used.Type: ApplicationFiled: March 31, 2012Publication date: November 14, 2013Applicant: GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH, CHINESE ACADEMY OF SCIENCESInventors: Duanqing Pei, Tao Wang
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Publication number: 20130196985Abstract: The heterocyclic alkynyl benzene compounds of formula (I), their pharmaceutically acceptable salts and stereoisomers thereof, as well as application in preparing drugs for preventing or treating tumors. The compounds can overcome the clinically induced resistance against Gleevec.Type: ApplicationFiled: June 3, 2011Publication date: August 1, 2013Applicant: Guangzzhou Institute of Biomedicine and Health, Chineses Academy of SciencesInventors: Ke Ding, Deping Wang, Duanqing Pei, Zhang Zhang, Mengjie Shen, Kun Luo, Yubing Feng
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Publication number: 20130040390Abstract: The present invention provides a chemically defined culture system, by which induced pluripotent stem (iPS) cells are obtained with high efficiency. The culture medium supplement of the present invention includes vitamin C and a glycogen synthase kinase-3 inhibitor; another culture medium supplement of the present invention further includes, in addition to vitamin C and the glycogen synthase kinase-3 inhibitor, vitamin B12, insulin, a receptor tyrosine kinase, and an anti-oxidant; and the culture medium supplement of the present invention may further be a mixture of the above two culture medium supplements with a serum replacement cell growth promoter. The present invention further provides a complete culture medium for iPS cells, which is formed by one or more of a basal culture medium, serum, and a serum replacement supplement, and the above culture medium supplements, or formed only by the above culture medium supplements and a basal culture medium.Type: ApplicationFiled: July 29, 2010Publication date: February 14, 2013Applicant: GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH, CHINESE ACADEMY OF SCIENCEInventors: Duanqing Pei, Jiekai Chen, Jing Liu
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Publication number: 20120100568Abstract: A serum-free medium for inducing and reprogramming somatic cells into induced pluripotent stem cells (iPS) quickly with high efficiency, and the method using thereof for inducing and reprogramming somatic cells without feeder are provided, wherein the rate and efficiency of whole process of inducing and reprogramming are greatly improved. The uses of the medium in inducing pluripotent stem cells, and the uses in the method for screening compounds, especially in the method for high throughput screening compounds are further provided.Type: ApplicationFiled: September 30, 2009Publication date: April 26, 2012Applicant: GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH, CHINESE ACADEMY OF SCIENCESInventors: Duanqing Pei, Jiekai Chen, Jing Liu