Abstract: A method for retrieving nucleic acid fragments, including: providing reduced DNA libraries during sequencing; amplifying nucleic acid fragments from the reduced DNA libraries; and retrieving the amplified nucleic acid fragments. The method further includes acquiring desired nucleic acid fragments among the retrieved nucleic acid fragments. The desired nucleic acid fragments are acquired using sequences having a homology to the barcode sequences, when the amplified nucleic acid fragments are tagged with specific barcode sequences.

Abstract: Disclosed is a method of preparing nucleic acid molecules, including: providing a pool of oligonucleotides, each containing restriction enzyme digestion sequences and generic flanking sequences; cleaving the restriction enzyme digestion sequence portions to provide a pool of mixtures comprising the oligonucleotides, each containing the generic flanking sequences at one end, and the oligonucleotides, each containing none of the generic flanking sequences at one end, and; assembling the oligonucleotides using the generic flanking sequences to randomly synthesize nucleic acid fragments.

Abstract: Provided is a method for stably synthesizing an error-free gene using a high-depth oligonucleotide tiling, which includes designing an oligonucleotide fragment by an over-overlapping method, synthesizing the oligonucleotide fragment using DNA microarray, retrieving error-free oligonucleotide fragments retrieved by next generation sequencing, and assembling the error-free oligonucleotide fragments.

Abstract: Proposed is a method of easily finding an error during analysis of various library sequences of nucleic acid base sequences after synthesizing a gene library using a combination of three nucleic acid base sequences (codon) translated into the same protein. This shows that it is possible to create a gene library having the same protein sequence but different nucleic acid base sequences. The present disclosure provides a novel experimental method capable of measuring a correlation between gene expression according to change in a codon by changing the usage of a codon optimized to express a specific gene in vivo.

Abstract: Provided is a method for stably synthesizing an error-free gene using a high-depth oligonucleotide tiling, which includes designing an oligonucleotide fragment by an over-overlapping method, synthesizing the oligonucleotide fragment using DNA microarray, retrieving error-free oligonucleotide fragments retrieved by next generation sequencing, and assembling the error-free oligonucleotide fragments.

Abstract: The present invention relates to a method of capturing a target nucleic acid sequence in genome sequencing, e using a CRISPR system. According to the present invention, the use of a plurality of CRIPSR systems enables capturing a plurality of target nucleic acids within genome simultaneously.

Abstract: Disclosed is a method of preparing nucleic acid molecules, including: providing a pool of oligonucleotides, each containing restriction enzyme digestion sequences and generic flanking sequences; cleaving the restriction enzyme digestion sequence portions to provide a pool of mixtures comprising the oligonucleotides, each containing the generic flanking sequences at one end, and the oligonucleotides, each containing none of the generic flanking sequences at one end, and; assembling the oligonucleotides using the generic flanking sequences to randomly synthesize nucleic acid fragments.

Abstract: A method for retrieving nucleic acid fragments, including: providing reduced DNA libraries during sequencing; amplifying nucleic acid fragments from the reduced DNA libraries; and retrieving the amplified nucleic acid fragments. The method further includes acquiring desired nucleic acid fragments among the retrieved nucleic acid fragments. The desired nucleic acid fragments are acquired using sequences having a homology to the barcode sequences, when the amplified nucleic acid fragments are tagged with specific barcode sequences.

Abstract: Provided is a method of preparing nucleic acid molecules comprising: (a) a step of providing nucleic acid fragments constituting at least a portion of the complete sequence of a target nucleic acid; (b) tagging the nucleic acid fragments with barcode sequences; (c) identifying the sequence of the nucleic acid fragments tagged by the barcode sequences; and (d) recovering desired nucleic acid fragments among the sequence-identified nucleic acid fragments using the barcode sequences.

Abstract: Proposed is a method of easily finding an error during analysis of various library sequences of nucleic acid base sequences after synthesizing a gene library using a combination of three nucleic acid base sequences (codon) translated into the same protein. This shows that it is possible to create a gene library having the same protein sequence but different nucleic acid base sequences. The present disclosure provides a novel experimental method capable of measuring a correlation between gene expression according to change in a codon by changing the usage of a codon optimized to express a specific gene in vivo.

Abstract: Provided is a method of inserting a target sequence into a genome of a cell through positive selection or negative selection based on whether a marker is expressed.

Abstract: Provided is a method of preparing nucleic acid molecules comprising: (a) a step of providing nucleic acid fragments constituting at least a portion of the complete sequence of a target nucleic acid; (b) tagging the nucleic acid fragments with barcode sequences; (c) identifying the sequence of the nucleic acid fragments tagged by the barcode sequences; and (d) recovering desired nucleic acid fragments among the sequence-identified nucleic acid fragments using the barcode sequences.

Abstract: Methods and compositions for generating oligonucleotide pools are provided. Methods and compositions for enriching target nucleic acid sequences are also provided.

Abstract: The present invention concerns methods and compositions for synthesizing a polypeptide using kinetically controlled reactions involving fragments of the polypeptide for a fully convergent process. In more specific embodiments, a ligation involves reacting a first peptide having a protected cysteyl group at its N-terminal and a phenylthioester at its C-terminal with a second peptide having a cysteine residue at its N-termini and a thioester at its C-termini to form a ligation product. Subsequent reactions may involve deprotecting the cysteyl group of the resulting ligation product and/or converting the thioester into a thiophenylester.

Abstract: The present invention concerns methods and compositions for synthesizing a polypeptide using kinetically controlled reactions involving fragments of the polypeptide for a fully convergent process. In more specific embodiments, a ligation involves reacting a first peptide having a protected cysteyl group at its N-terminal and a phenylthioester at its C-terminal with a second peptide having a cysteine residue at its N-termini and a thioester at its C-termini to form a ligation product. Subsequent reactions may involve deprotecting the cysteyl group of the resulting ligation product and/or converting the thioester into a thiophenylester.