Abstract: In flow cytometry, particles (2) can be distinguished between populations (8) by combining n-dimensional parameter data, which may be derived from signal data from a particle, to mathematically achieve numerical results representative of an alteration (48). An alteration may include a rotational alteration, a scaled alteration, or perhaps even a translational alteration. Alterations may enhance separation of data points which may provide real-time classification (49) of signal data corresponding to individual particles into one of at least two populations.

Abstract: An eaves trough cleaner including a fluid flow mechanism. The fluid flow mechanism has a top end and a bottom end and an intermediate portion with a cross tubing integral therewith. The cross tubing has an internal fluid passage and a second end with a container coupled. The internal passage has a vertical extension coupled to an elongated tube positionable within the container. Included is a trigger mechanism with a regulator valve coupled to the intermediate portion and spaced from the cross tubing. Also, a cleaning tool is provided. Lastly, at least one connector pipe member is included. The one connector pipe member has an upper end and a bottom end. The bottom end of the pipe member couples with the fluid flow mechanism. The upper end of the pipe member couples with the cleaning tool for placement of the cleaning tool within the eaves trough.