Abstract: A system and method for performing speckle correlation flow cytometry (SCFC). By subtracting out the stationary background when shining light through a sample (e.g., a vessel within a biological tissue), light only scattered by the desired targets (e.g., cells) can be captured and different types of targets (e.g., cells) can be distinguished by the autocorrelation of the speckle pattern. In this way, the targets (e.g., cells) can be classified and counted based on the features of their speckle correlations. The technique can be applied not only for noninvasive, label-free, in vivo CTC counting but also for counting other types of blood cells such as white blood cells or red blood cells.

Abstract: A method of imaging an object on one or more sensor pixels and with reduced glare. The method includes irradiating a scattering medium and the object behind the scattering medium, creating backscattered radiation and imaging radiation that are received on the one or more pixels. The method includes digitally adjusting a phase, an amplitude, or a phase and amplitude, of reference radiation transmitted onto the one or more sensor pixels, wherein the reference radiation destructively interferes with the backscattered radiation (glare) on the one or more sensor pixels while the object is imaged on the one or more sensor pixels using the imaging radiation.

Abstract: A system and method for performing speckle correlation flow cytometry (SCFC). By subtracting out the stationary background when shining light through a sample (e.g., a vessel within a biological tissue), light only scattered by the desired targets (e.g., cells) can be captured and different types of targets (e.g., cells) can be distinguished by the autocorrelation of the speckle pattern. In this way, the targets (e.g., cells) can be classified and counted based on the features of their speckle correlations. The technique can be applied not only for noninvasive, label-free, in vivo CTC counting but also for counting other types of blood cells such as white blood cells or red blood cells.

Abstract: A method of imaging an object on one or more sensor pixels and with reduced glare. The method includes irradiating a scattering medium and the object behind the scattering medium, creating backscattered radiation and imaging radiation that are received on the one or more pixels. The method includes digitally adjusting a phase, an amplitude, or a phase and amplitude, of reference radiation transmitted onto the one or more sensor pixels, wherein the reference radiation destructively interferes with the backscattered radiation (glare) on the one or more sensor pixels while the object is imaged on the one or more sensor pixels using the imaging radiation.