Patents by Inventor Edward R. LaVallie

Edward R. LaVallie has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Secreted proteins and polynucleotides encoding them
    Patent number: 5728819
    Abstract: Novel polynucleotides and the proteins encoded thereby are disclosed.
    Type: Grant
    Filed: August 2, 1996
    Date of Patent: March 17, 1998
    Assignee: Genetics Institute, Inc.
    Inventors: Kenneth Jacobs, John M. McCoy, Edward R. LaVallie, Lisa A. Racie, David Merberg, Maurice Treacy, Cheryl Evans, Vikki Spaulding
  • Secreted proteins and polynucleotides encoding them
    Patent number: 5723315
    Abstract: Novel polynucleotides and the proteins encoded thereby are disclosed.
    Type: Grant
    Filed: August 23, 1996
    Date of Patent: March 3, 1998
    Assignee: Genetics Institute, Inc.
    Inventors: Kenneth Jacobs, John M. McCoy, Edward R. LaVallie, Lisa A. Racie, David Merberg, Maurice Treacy, Vikki Spaulding
  • Secreted proteins and polynucleotides encoding them
    Patent number: 5708157
    Abstract: Novel polynucleotides and the proteins encoded thereby are disclosed.
    Type: Grant
    Filed: July 26, 1996
    Date of Patent: January 13, 1998
    Assignee: Genetics Institute, Inc.
    Inventors: Kenneth Jacobs, John M. McCoy, Edward R. LaVallie, Lisa A. Racie, David Merberg, Maurice Treacy, Cheryl Evans, Vikki Spaulding
  • Cloning of enterokinase and method of use
    Patent number: 5665566
    Abstract: Provided are nucleic acid sequence sequences encoding enterokinase activity, the expression products thereof, and methods for using same.
    Type: Grant
    Filed: February 23, 1994
    Date of Patent: September 9, 1997
    Assignee: Genetics Institute, Inc.
    Inventor: Edward R. LaVallie
  • Secreted proteins and polynucleotides encoding them
    Patent number: 5654173
    Abstract: Novel polynucleotides and the proteins encoded thereby are disclosed.
    Type: Grant
    Filed: August 23, 1996
    Date of Patent: August 5, 1997
    Assignee: Genetics Institute, Inc.
    Inventors: Kenneth Jacobs, John M. McCoy, Edward R. LaVallie, Lisa A. Racie, David Merberg, Maurice Treacy, Vikki Spaulding
  • Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules
    Patent number: 5646016
    Abstract: Provided is a fusion molecule comprising a DNA sequence encoding a thioredoxin-like protein fused to a DNA sequence encoding a second peptide or protein. The peptide or protein may be fused to the amino terminus of the thioredoxin-like molecule, the carboxyl terminus of the thioredoxin-like molecule, or within the thioredoxin-like molecule, for example at the active-site loop of said molecule. The fusion molecule may be modified to introduce one or more metal-binding/chelating amino-acid residues to aid in purification. Expression of this fusion molecule under the control of a regulatory sequence capable of directing its expression in a desired host cell, produces high levels of stable and soluble fusion protein. The fusion protein, located in the bacterial cytoplasm, may be selectively released from the cell by osmotic shock or freeze/thaw procedures. It may be optionally cleaved to liberate the soluble, correctly folded heterologous protein from the thioredoxin-like portion.
    Type: Grant
    Filed: December 10, 1993
    Date of Patent: July 8, 1997
    Assignee: Genetics Institute, Inc.
    Inventors: John McCoy, Elizabeth DiBlasio-Smith, Kathleen Grant, Edward R. LaVallie
  • Peptide and protein fusions to thioredoxin and thioredoxin-like molecules
    Patent number: 5292646
    Abstract: This invention provides a fusion molecule comprising a DNA sequence encoding a thioredoxin-like protein fused to the DNA sequence encoding a selected heterologous peptide or protein. The peptide or protein may be fused to the amino terminus of the thioredoxin-like molecule, the carboxyl terminus of the thioredoxin-like molecule, or within the thioredoxin-like molecule, for example at the active-site loop of said molecule. Expression of this fusion molecule under the control of a regulatory sequence capable of directing its expression in a desired host cell, produces high levels of stable and soluble fusion protein. The fusion protein, located in the bacterial cytoplasm, may be selectively released from the cell by osmotic shock or freeze/thaw procedures. It may be optionally cleaved to liberate the soluble, correctly folded heterologous protein from the thioredoxin-like portion.
    Type: Grant
    Filed: July 28, 1992
    Date of Patent: March 8, 1994
    Assignee: Genetics Institute, Inc.
    Inventors: John McCoy, Edward R. LaVallie
  • Peptide and protein fusions to thioredoxin and thioredoxin-like molecules
    Patent number: 5270181
    Abstract: This invention provides a fusion molecule comprising a DNA sequence encoding a thioredoxin-like protein fused to the DNA sequence encoding a selected heterologous peptide or protein. The peptide or protein may be fused to the amino terminus of the thioredoxin-like molecule, the carboxyl terminus of the thioredoxin-like molecule, or within the thioredoxin-like molecule, for example at the active-site loop of said molecule. Expression of this fusion molecule under the control of a regulatory sequence capable of directing its expression in a desired host cell, produces high levels of stable and soluble fusion protein. The fusion protein, located in the bacterial cytoplasm, may be selectively released from the cell by osmotic shock or freeze/thaw procedures. It may be optionally cleaved to liberate the soluble, correctly folded heterologous protein from the thioredoxin-like portion.
    Type: Grant
    Filed: August 14, 1991
    Date of Patent: December 14, 1993
    Assignee: Genetics Institute, Inc.
    Inventors: John McCoy, Edward R. LaVallie
  • Method for producing heterologous proteins
    Patent number: 4801536
    Abstract: This invention concerns a method for producing a heterologous protein in a bacterial host cell such that the protein is exported from the host cell into the culture medium. The method involves culturing in a bacterial culture medium a genetically engineered bacterial strain containing a fusion DNA sequence comprising a first nucleotide sequence encoding at least an N-terminal portion of a flagellin protein and a second nucleotide sequence encoding the heterologous protein. The first nucleotide sequence is linked via its 3' terminus to the 5' terminus of the second nucleotide sequence, and the fusion DNA sequence is itself linked to an expression control sequence.
    Type: Grant
    Filed: June 2, 1987
    Date of Patent: January 31, 1989
    Assignee: Genetics Institute, Inc.
    Inventors: Mark L. Stahl, Edward R. LaVallie