Patents by Inventor Eero Mustalahti

Eero Mustalahti has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
    Patent number: 10513724
    Abstract: The present disclosure relates to compositions and methods useful for the production of recombinant glycoproteins in filamentous fungal cells, such as Trichoderma cells, wherein at least 90% (mol %), preferably at least 95% of the total neutral N-glycans of said produced recombinant glycoprotein are mammalian-like N-glycans. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutations that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s); ii. a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase; iii. a recombinant polynucleotide for increasing ?1, 2 mannosidase activity;and, iv. a recombinant polynucleotide encoding said heterologous glycoprotein.
    Type: Grant
    Filed: July 21, 2015
    Date of Patent: December 24, 2019
    Assignee: GLYKOS FINLAND OY
    Inventors: Jari Natunen, Anne Leppänen, Heidi Salminen, Jukka Hiltunen, Anne Kanerva, Annamari Heiskanen, Ann Westerholm-Parvinen, Georg Schmidt, Anne Huuskonen, Eero Mustalahti, Christopher Landowski, Markku Saloheimo, Juhani Saarinen, Benjamin Patrick Sommer, Ramon Wahl, Christian Ostermeier, Bernhard Helk
  • Concentration and purification of hydrophobins and antibodies with a phase separation method
    Patent number: 10266562
    Abstract: The present invention relates to a purification and concentration method for proteins and antibodies. Particularly the present invention relates to a continuous surfactant based phase separation method for recovering hydrophobin fusion proteins, and for recovering target molecules, such as antibodies, directly from a liquid by using phase separation and hydrophobin-Protein A fusion technologies.
    Type: Grant
    Filed: July 1, 2014
    Date of Patent: April 23, 2019
    Assignee: Teknologian tutkimuskeskus VTT Oy
    Inventors: Jussi Joensuu, Markus Linder, Eero Mustalahti, Katri Kurppa
  • PRODUCTION OF GLYCOPROTEINS WITH MAMMALIAN-LIKE N-GLYCANS IN FILAMENTOUS FUNGI
    Publication number: 20170159094
    Abstract: The present disclosure relates to compositions and methods useful for the production of recombinant glycoproteins in filamentous fungal cells, such as Trichoderma cells, wherein at least 90% (mol %), preferably at least 95% of the total neutral N-glycans of said produced recombinant glycoprotein are mammalian-like N-glycans. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutations that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s); ii.a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase; iii. a recombinant polynucleotide for increasing ?1, 2 mannosidase activity;and, iv. a recombinant polynucleotide encoding said heterologous glycoprotein.
    Type: Application
    Filed: July 21, 2015
    Publication date: June 8, 2017
    Applicant: GLYKOS FINLAND OY
    Inventors: Jari Natunen, Anne Leppänen, Heidi Salminen, Jukka Hiltunen, Anne Kanerva, Annamari Heiskanen, Ann Westerholm-Parvinen, Georg Schmidt, Anne Huuskonen, Eero Mustalahti, Christopher Landowski, Markku Saloheimo, Juhani Saarinen, Benjamin Patrick Sommer, Ramon Wahl, Christian Ostermeier, Bernhard Helk
  • Concentration and purification of hydrophobins and antibodies with a phase separation method
    Publication number: 20160159854
    Abstract: The present invention relates to a purification and concentration method for proteins and antibodies. Particularly the present invention relates to a continuous surfactant based phase separation method for recovering hydrophobin fusion proteins, and for recovering target molecules, such as antibodies, directly from a liquid by using phase separation and hydrophobin-Protein A fusion technologies.
    Type: Application
    Filed: July 1, 2014
    Publication date: June 9, 2016
    Inventors: Jussi Joensuu, Markus Linder, Eero Mustalahti, Katri Kurppa
  • Improving the production of foreign proteins
    Patent number: 9133494
    Abstract: The present invention concerns a method for improving the expression levels of proteins, wherein hydrophobin fusion proteins are expressed, thereby inducing the formation of protein bodies, and wherein said hydrophobin fusions are co-expressed with a target protein or with a further fusion of the target protein.
    Type: Grant
    Filed: June 29, 2012
    Date of Patent: September 15, 2015
    Assignee: Teknologian tutkimuskeskus VTT Oy
    Inventors: Jussi Joensuu, Eero Mustalahti, Andrew Conley
  • Improving the production of foreign proteins
    Publication number: 20140212926
    Abstract: The present invention concerns a method for improving the expression levels of proteins, wherein hydrophobin fusion proteins are expressed, thereby inducing the formation of protein bodies, and wherein said hydrophobin fusions are co-expressed with a target protein or with a further fusion of the target protein.
    Type: Application
    Filed: June 29, 2012
    Publication date: July 31, 2014
    Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTT
    Inventors: Jussi Joensuu, Eero Mustalahti, Andrew Conley