Abstract: The invention relates to the purification of bispecific antibodies carrying a different specificity for each binding site of the immunoglobulin molecule from a mixture of monospecific antibodies. The bispecific antibodies are composed of a single heavy chain and two different light chains, one containing a Kappa constant domain and the other a Lambda constant domain. This invention in particular relates to the isolation of these bispecific antibodies from mixtures that contain monospecific antibodies having two Kappa light chains or portions thereof and monospecific antibodies having two Lambda light chains or portions thereof. The invention also provides the methods of efficiently purifying these bispecific antibodies.

Abstract: The invention relates to the purification of bispecific antibodies carrying a different specificity for each binding site of the immunoglobulin molecule from a mixture of monospecific antibodies. The bispecific antibodies are composed of a single heavy chain and two different light chains, one containing a Kappa constant domain and the other a Lambda constant domain. This invention in particular relates to the isolation of these bispecific antibodies from mixtures that contain monospecific antibodies having two Kappa light chains or portions thereof and monospecific antibodies having two Lambda light chains or portions thereof. The invention also provides the methods of efficiently purifying these bispecific antibodies.

Abstract: A separating material formed by a solid substrate having a substrate surface, primary or secondary amines coupled to the substrate surface, and a graft polymer formed on the substrate by covalently coupling the primary or secondary amines with a thermally labile radical initiator and subsequently contacting the substrate surface with a solution of one or more polymerizable monomers. Methods for the extracorporeal treatment of blood, blood plasma or blood serum employing the separating material, for affinity adsorption, ion-exchange adsorption, hydrophobic adsorption, or hydrophilic adsorption employing the separating material, and a separating column employing the separating material are also disclosed.

Abstract: A separating material formed by a solid substrate having a substrate surface, primary or secondary amines coupled to the substrate surface, and a graft polymer formed on the substrate by covalently coupling the primary or secondary amines with a thermally labile radical initiator and subsequently contacting the substrate surface with a solution of one or more polymerizable monomers. Methods for the extracorporeal treatment of blood, blood plasma or blood serum employing the separating material, for affinity adsorption, ion-exchange adsorption, hydrophobic adsorption, or hydrophilic adsorption employing the separating material, and a separating column employing the separating material are also disclosed.

Abstract: The present invention provides a separating material producable by a) providing a solid substrate, having amino-functional groups coupled to the substrate surface, b) covalently coupling of the amino-functional groups with a thermally labile radical initiator, c) contacting the substrate surface with a solution of polymerizable monomers under conditions, where thermally initiated graft copolymerization of the monomers takes place, to form a structure of adjacent functional polymer chains on the surface of the substrate.

Abstract: The present invention provides a separating material producable by a) providing a solid substrate, having amino-functional groups coupled to the substrate surface, b) covalently coupling of the amino-functional groups with a thermally labile radical initiator, c) contacting the substrate surface with a solution of polymerizable monomers under conditions, where thermally initiated graft copolymerization of the monomers takes place, to form a structure of adjacent functional polymer chains on the surface of the substrate.

Abstract: The invention relates to a process for the production of chromatography columns or capillaries containing sorbents of monolithic mouldings which can remain directly in their gelation mould after production. This is achieved by the process according to the invention, in which the gelation mould is repeatedly filled with the monomer sol.

Abstract: The invention relates to methods for separating biological contaminants (viruses, nucleic acids and/or endotoxins) from biological products, or for reducing the content of such biological contaminants in said biological products. The sample (biological product) to be purified is treated with an anion exchanger membrane. Said anion exchanger membrane is obtained by reacting a polyamide with an aminoreactive compound which is capable of polymerisation. This is followed by polymerisation with monomers which contain cationic groups or into which cationic groups can be introduced in a reaction analogous to polymerisation.

Abstract: The present invention relates to a process for removing an initiator of cerium (IV) salt from a graft product. The process may include washing the graft product with a mineral acid solution comprising ascorbic acid.

Abstract: The present invention relates to an azlactone-derivatized polyamide shaped article made by reacting at least one polyamide with a solution comprising an aza-bicyclo compound and a vinylazlactone derivative of the formula I wherein R1, R2, R3, R4, and R5 are defined herein.

Abstract: The invention concerns the preparation of azlactone-derivatized polyamides which are obtained by reacting polyamides with a solution containing an azabicyclo compound and a vinyl azlactone derivative of formula (I), in which R1, R2 and R3, independently of one another, designate H or CH3; and R4 and R5, independently of each other, designate H or C1 to C3 alkyl. These azlactone-derivatized polyamides can be used to bond amino-group-containing compounds to a matrix. Examples of these polyamides include affinity carriers and immobilized enzymes.

Abstract: Activated support materials are provided containing oxirane or azlactone groups as substituents in linear polymers as activated groups. A base support containing hydroxyl groups is suspended in a solution containing cerium (IV) ions and a monomer containing an oxirane or azlactone group, and grafting polymerization is carrier out to produce a polymer containing oxirane or azlactone groups covalently bonded to the base support. Azlactone groups can be bonded to the base support via a thioether bond by using a base support containing thiol groups. The activated support materials can be used to prepare affinity supports containing an affinity ligand that is thiophilic or possesses a metal chelating group, or to prepare immobilized enzymes. The ligand can be iminodiacetic acid, or can be obtained by reacting an oxirane group of the support material with NaHS, and reacting the resultant product with divinylsulfone followed by reacting with mercaptoethanol.

Abstract: The invention relates to separating materials based on supports containing hydroxyl groups, whose surface [sic] are coated with covalently bound polymers, and to processes for their preparation. The separating materials are characterized in that the polymers consist of identical recurring units of the formula I.brket open-st.CH.sub.2 --CHX.brket close-st..sub.n (I)in whichX is CO--NH--CH.sub.2 --CH.sub.2 --SO.sub.3 H andn is 2-100.

Abstract: The invention relates to polymerisable double-bond derived polyamides obtainable by reacting the polyamide in an aqueous solution with a compound which contains both a polymerisable double bond and an oxirane ring. These derived polyamides may be processed to provide graft polymers with improved properties.

Abstract: The invention relates to activated support materials which comprise epoxy-substituted poly(meth)acrylamides, characterized in that:a) the base support contains aliphatic hydroxyl groups,b) the covalently bonded polymers are bonded to the support via a terminal monomer unit,c) the polymers contain monomer units of the formula I,d) the monomer units are linearly linked, ##STR1## in which R.sup.1, R.sup.2 and R.sup.3 independently of one another are H or CH.sub.3,R.sup.4 is H or alkyl having 1-5 carbon atomsandn is an integer between 1 and 5 and to the use thereof.

Abstract: The invention relates to sorbents for for ?sic! affinity chromatography which are based on hydroxyl-containing base supports on whose surfaces polymers are covalently bonded, characterized in thata) the base support contains aliphatic hydroxyl groups,b) the covalently bonded polymers are bound to the base support by a terminal monomer unit,c) the polymers contain monomer units of the formula IId) the monomer units are linked linearly, ##STR1## in which R.sup.1, R.sup.2 and R.sup.3 independently of one another are H or CH.sub.3,R.sup.4 is H, C.sub.1 -C.sub.5 -alkyl or C.sub.6 -C.sub.12 -aryl,n is an integer between 1 and 5 andone of the radicals X is OH and the other radical X is a nucleoside-containing radical,and to their preparation and use, especially for affinity chromatography.

Abstract: The invention relates to an improved method for gel permeation chromatography and to support materials for this method based on hydroxyl-containing base supports onto whose surfaces polymers are covalently bonded, whereina) the base support comprises aliphatic hydroxyl groups,b) the covalently bonded polymers are bonded to the support via a terminal monomer unit,c) the linear polymers comprise monomer units of formula II,d) the polymers are crosslinked by ether bridges ##STR1## in which R.sup.1, R.sup.2 and R.sup.3 independently of one another are H or CH.sub.3,R.sup.4 is H or C.sub.1 -C.sub.5 -alkyl andn is an integer between 1 and 5.

Abstract: The invention relates to separation materials for ion exchange chromatography which are based on hydroxyl-containing base supports on whose surfaces polymers are covalently bonded, characterized in thata) the base support contains aliphatic hydroxyl groups,b) the covalently bonded polymers are bonded to the base support via a terminal monomer unit,c) the polymers contain monomer units of both the formula II and the formula III,d) the monomer units are linked linearly, ##STR1## in which R.sup.1, R.sup.2 and R.sup.3 independently of one another are H or CH.sub.3,R.sup.4 is H, C.sub.1 -C.sub.5 -alkyl or C.sub.6 -C.sub.12 -aryl,n is an integer between 1 and 5,one radical X is OH and the other radical X is NR.sup.5 R.sup.6, N.sup.+ R.sup.5 R.sup.6 R.sup.7 or SO.sub.3 H, andR.sup.5, R.sup.6 and R.sup.7 independently of one another are C.sub.1 -C.sub.4 -alkyl, it also being possible for one or both radicals R.sup.5 and/or R.sup.6 to be H.

Abstract: The invention concerns stationary phases for use in hydrophobic interaction chromatography, the stationary phases being based on hydroxy-group-containing support materials on whose surfaces polymers are covalently bound. The stationary phases are characterized in that:a) the support material contains aliphatic hydroxy groups;b) the polymers are covalently bound to the support material through a terminal monomer unit;c) the polymers contain monomer units of formula (II) ##STR1## in which R.sup.1, R.sup.2 and R.sup.3 are, independently of each other, H or CH.sub.3,R.sup.4 is H, C.sub.1 -C.sub.5 -alkyl or C.sub.6 -C.sub.12 -aryl,n is an integer between 1 and 5,one X radical is OH and the other X radical is Z--R.sup.5,Z is O or NH,andR.sup.5 is C.sub.1 -C.sub.20 -alkyl, C.sub.6 -C.sub.25 -aryl, C.sub.7 -C.sub.25 -alkylaryl or C.sub.7 -C.sub.25 -arylalkyl which is optionally derivatized, once or more than once, with nitrile or C.sub.1 -C.sub.5 -alkoxy, where one or more non-adjacent CH.sub.

Abstract: A dual-circuit pressure medium brake system is equipped with relay valves disposed ahead of the brake cylinders. Each brake circuit is equipped with a control also acting in the other circuit and overlapping each other, and a primary brake circuit is designed in such a way that it contains all control elements necessary for optimal brake pressure adaptation, while a secondary brake circuit is merely an auxiliary brake circuit without control elements. In this manner each brake pressure metering can be simultaneously adjusted. During the loss of the primary brake circuit, the secondary brake circuit guarantees a minimal brake pressure. Such a pressure medium brake system is preferably used in air brake systems of motor vehicles.