Abstract: Polymeric contrast agents are used to monitor myocardial neovascularization. The agent is injected and images of the myocardium are obtained at various time intervals after injection. Localized image enhancement indicates an area of increased angiogenesis.

Abstract: Polymeric contrast agents are used to monitor myocardial neovascularization. The agent is injected and images of the myocardium are obtained at various time intervals after injection. Localized image enhancement indicates an area of increased angiogenesis.

Abstract: Sequential injections of contrast agents are employed for assessing angiogenic activity. At least one of the injections is of a polymeric contrast agent which binds to angiogenic microvasculature.

Abstract: Polymeric contrast agents are used to test for blood vessel permeability, such as, for example, to diagnose sepsis. The agent is injected and images are obtained at various time intervals after injection. For increased accuracy, the blood circulation levels of the agents may optionally be monitored.

Abstract: A contrast agent for magnetic resonance imaging is provided which is initially substantially neutral or negatively charged and which is acted upon by one or more enzymes to create a paramagnetic metal containing probe which is positively charged. The enzyme activated contrast agent localizes in areas containing the enzyme(s).

Abstract: A biocompatible molecule includes a polypeptide containing lysine residues and either gluatmic acid or aspartic acid residues, less than 90% of the lysine residues being substituted with a group derived from a steric hindrance molecule, the substituted polypeptide having a conformation with a length that is 5 to 500 times its average diameter.

Abstract: Improved methods for forming substituted polymers having a high degree of conjugation include the steps of activating a steric hindrance molecule under conditions that ensure substantially all the steric hindrance molecules are mono-activated and then reacting with a polymer. An improved method for recovering polypeptides substituted with steric hindrance molecules and having an elongate configuration removes impurities and volatiles without achieving complete dryness of the copolymer.

Abstract: Thermal drug treatment of tumor tissue is obtained by attaching a thermally active drug to carrier molecules which have an affinity to tumor tissue. Localized heating is performed on the tumor tissue, thereby activating the drug in the tumor tissue. The end result may be concentrated delivery of a drug to a chosen tissue, or, in the case where the drug creates a toxin when heated, selective tissue destruction of a selected locations heated. The localized heat may be applied by focused ultrasound heating.

Abstract: An in-situ method of extracting contaminants from a soil volume comprises applying a radiofrequency (RF) excitation signal to heat the soil with an array of electrodes. The electrodes are inserted into the contaminated volume or inserted into a matrix of holes drilled into the volume. A first row of electrodes is electrically coupled to a shield of a coaxial cable, with a second row electrically coupled to the central conductor of the coaxial cable. RF energy is applied to pairs of electrode rows through the coaxial cable and a matching network is installed in front of the electrode-row pair to maximize power flow into the electrode-row pair. This results in very evenly distributed voltages which results in even heating. A balanced-to-unbalanced transformer (balun) is installed at the input to the matching network to prevent the deposition of RF energy outside the target volume and creation of voltages that could be hazardous to personnel.

Abstract: A method is provided for forming ordered macromolecular protein arrays by avoiding a binding pathway that leads to densely packed, disordered states during protein crystal growth, by a diffusion limited process or by allowing controlled growth to occur by removal of the initial supported protein layer to a different environment.

Abstract: A method is provided for making ordered monolayers of macromolecules. A supported lipid polylayer is contacted with macromolecules in an aqueous polar solution and allowed to incubate.

Abstract: By means of a thin, porous polymer overcoating, the surface of a silver-silver chloride reversible electrode is adequately stabilized for use at high current densities in electrophoretic analysis applications.

Abstract: In a mixture of two antibody-coated latex particle suspensions, the particles in each suspension being of a different size, respectively, absence of antigen specific to the antibody in the mixture leaves multiplets only of one and the other sizes of particles. Presence of antigen in the mixture results in large particle/small particle coupling, which is detected by the resistive pulse method and indicates that an antigen-antibody reaction has occurred.

Abstract: An immune assay for tracing processes and reactions employs a narrow band laser tuned to the absorption line of a tracer isotope coupled chemically to a carrier molecule of protein. The carrier molecule is reacted with another molecule, the reaction products are vaporized, and resonance fluorescence from the tracer isotope illuminated by the laser is measured. Amplitude of the fluorescence signal provides a direct measure of the number of isotope-tagged molecules present after the reaction of interest.

Abstract: Polystyrene particles having attached antigen and high electrophoretic mobility, and polystyrene particles having attached antigen and low electrophoretic mobility, when paired in a common solution with antibodies specific to the antigen, give rise to a particle population of intermediate electrophoretic mobility when measured by the Doppler shift of scattered laser light, thereby signaling presence of the antibodies in the solution.

Abstract: Changes in electrophoretic mobility distribution of particles in an electric field, as determined by Doppler shifts of laser light scattered by the particles while undergoing oscillation, may be unambiguously measured when the electric field is produced by a frequency modulated square wave voltage. Diminished likelihood of potential ambiguities in the measurement is achieved by smoothing the scattered light spectra and reducing harmonic spectral structures.

Abstract: Effectiveness on cellular processes of a drug or antibiotic introduced into isotonic sucrose solution of 0.005 Normal sodium chloride containing physiological cells in suspension is determined by irradiating the solution, situated in an electric field, with a laser beam and measuring frequency difference between incident light and light scattered by cell motion in the electric field.

Abstract: Reduction in electrophoretic mobility of antigen-coated microscopic polystyrene spheres due to an antigen-antibody reaction is detected by observing changes in oscillation amplitude of speckle patterns (i.e., regions of light and dark) produced by laser light scattered by the particles as they are driven back and forth by a square wave electric field.

Abstract: A method of rapidly detecting presence of antibodies in a solution comprises depositing, on each of a plurality of microscopic particles, an antigen specific to the antibodies sought, and forming a dilute suspension of the particles in the solution to be examined. The suspension is stirred, and electrophoretic mobility of the particles is measured upon formation of the suspension and measured again at a subsequent time. Detection of a change in electrophoretic mobility of the particles between the two measurements indicates presence of the antibodies in the solution.