Abstract: A method for increasing the amount of trans-1,4-polyisoprene contained in a plant, and a method for effectively producing trans-1,4-polyisoprene using a plant are provided. A long-chain trans-prenyl diphosphate synthase gene that comprises DNA having at least one base sequence selected from the group consisting of a base sequence from positions 88 to 1134 of the base sequence of SEQ ID NO: 1 or a complementary sequence thereof, a base sequence from positions 42 to 1088 of the base sequence of SEQ ID NO: 3 or a complementary sequence thereof, and a base sequence from positions 91 to 1140 of the base sequence of SEQ ID NO: 5 or a complementary sequence thereof are disclosed; as well as a plant transformed with an expression vector containing the gene.

Abstract: According to this invention, a gene cluster involved in the non-mevalonate pathway of Hevea brasiliensis was obtained and nucleotide sequences of these genes were determined. The gene cluster according to this invention involved in the IPP biosynthesis in the non-mevalonate pathway is involved in the biosynthesis of vitamin E and carotenoids. Therefore, the Hevea brasiliensis obtained by introducing the gene cluster of the present invention can be expected to produce high-quality rubber with improved permanence.

Abstract: According to the present invention, a gene cluster encoding various kinds of prenyl transferase of Hevea brasiliensis is obtained, and the nucleotide sequences of these genes are determined. The prenyl transferases are involved in the biosynthesis of isoprene backbone of natural rubber. Therefore, improvement of productivity of rubber can be expected, by transforming plants by the gene cluster obtained in the present invention.

Abstract: According to this invention, a gene cluster involved in the non-mevalonate pathway of Hevea brasiliensis was obtained and nucleotide sequences of these genes were determined. The gene cluster according to this invention involved in the IPP biosynthesis in the non-mevalonate pathway is involved in the biosynthesis of vitamin E and carotenoids. Therefore, the Hevea brasiliensis obtained by introducing the gene cluster of the present invention can be expected to produce high-quality rubber with improved permanence.

Abstract: According to this invention, a gene cluster involved in the non-mevalonate pathway of Hevea brasiliensis was obtained and nucleotide sequences of these genes were determined. The gene cluster according to this invention involved in the IPP biosynthesis in the non-mevalonate pathway is involved in the biosynthesis of vitamin E and carotenoids. Therefore, the Hevea brasiliensis obtained by introducing the gene cluster of the present invention can be expected to produce high-quality rubber with improved permanence.

Abstract: A method for increasing the amount of trans-1,4-polyisoprene contained in a plant, and a method for effectively producing trans-1,4-polyisoprene using a plant are provided. A long-chain trans-prenyl diphosphate synthase gene that comprises DNA having at least one base sequence selected from the group consisting of a base sequence from positions 88 to 1134 of the base sequence of SEQ ID NO: 1 or a complementary sequence thereof, a base sequence from positions 42 to 1088 of the base sequence of SEQ D NO: 3 or a complementary sequence thereof, and a base sequence from positions 91 to 1140 of the base sequence of SEQ ID NO: 5 or a complementary sequence thereof are disclosed; as well as a plant transformed with an expression vector containing the gene.

Abstract: According to the present invention, a gene cluster encoding various kinds of prenyl transferase of Hevea brasiliensis is obtained, and the nucleotide sequences of these genes are determined. The prenyl transferases are involved in the biosynthesis of isoprene backbone of natural rubber. Therefore, improvement of productivity of rubber can be expected, by transforming plants by the gene cluster obtained in the present invention.

Abstract: According to the present invention, a gene cluster encoding various kinds of prenyl transferase of Hevea brasiliensis is obtained, and the nucleotide sequences of these genes are determined. The prenyl transferases are involved in the biosynthesis of isoprene backbone of natural rubber. Therefore, improvement of productivity of rubber can be expected, by transforming plants by the gene cluster obtained in the present invention.

Abstract: According to the present invention, a gene cluster encoding various kinds of prenyl transferase of Hevea brasiliensis is obtained, and the nucleotide sequences of these genes are determined. The prenyl transferases are involved in the biosynthesis of isoprene backbone of natural rubber. Therefore, improvement of productivity of rubber can be expected, by transforming plants by the gene cluster obtained in the present invention.

Abstract: According to this invention, a gene cluster involved in the non-mevalonate pathway of Hevea brasiliensis was obtained and nucleotide sequences of these genes were determined. The gene cluster according to this invention involved in the IPP biosynthesis in the non-mevalonate pathway is involved in the biosynthesis of vitamin E and carotenoids. Therefore, the Hevea brasiliensis obtained by introducing the gene cluster of the present invention can be expected to produce high-quality rubber with improved permanence.

Abstract: A method for introducing a foreign matter into a cell, includes the steps of placing a small particle carrying a foreign matter at a part of a cell surface of a living cell, boring a hole in a cell wall and/or a cell membrane by irradiating and treating said part of the cell surface with a laser beam, and introducing the foreign matter into the living cell.

Abstract: The present invention provides a method for obtaining an aptamer capable of specifically adsorbing to Verotoxin-1 produced by Enterohemorrhagic Escherichia coli by an in vitro selection method utilizing affinity chromatography, particularly, a method including use of an affinity column immobilizing thereon a peptide having an amino acid sequence of Verotoxin-1, for the affinity chromatography, and a single strand nucleic acid molecule, which is an aptamer capable of specifically adsorbing to Verotoxin-1.

Abstract: The present invention provides an aptamer capable of specifically adsorbing to bisphenol A suspected to be an endocrine disrupter as a target molecule, a method for obtaining an aptamer capable of specifically adsorbing to bisphenol A by an in vitro selection method utilizing affinity chromatography using a carrier immobilizing bisphenol A, particularly, a method including use of an antagonistic elution buffer containing an amphiprotic organic solvent for elution by the affinity chromatography, and a single-strand nucleic acid molecule which is an aptamer obtained thereby.

Abstract: There is provided an equipment for transforming plants which comprises: a microporous body having a surface on which a plant seed is germinated and grown into a plant body, wherein the plant seed is germinated and grown by absorbing an aqueous nutrition which is retained in communicating pores in the microporous body from the surface of the microporous body; and a carrier solution containing a gene with which the plant body is transformed, wherein the grown plant body is transformed by immersing it in the carrier solution according to an in planta method. According to the equipment for transforming plants of the present invention, a method for experimenting, investigating and developing higher plants can be conducted more exactly, conveniently, speedy and efficiently.

Abstract: A novel method for introduction of an exogenous substance or a physiologically active compound into cells is provided according to this invention. This method can realize introduction of an exogenous genetic substance or a physiologically active compound of large size with a large amount. Such substance is immobilized to beads of sphere fine particles having a particle size of 0.01 mm to 10 mm, and bio-active beads thus produced are introduced into cells. Bio-active beads comprising calcium alginate are particularly useful for the purpose of the present invention.

Abstract: The present invention provides a method for obtaining an aptamer capable of specifically adsorbing to Verotoxin-1 produced by Enterohemorrhagic Escherichia coli by an in vitro selection method utilizing affinity chromatography, particularly, a method including use of an affinity column immobilizing thereon a peptide having an amino acid sequence of Verotoxin-1, for the affinity chromatography, and a single strand nucleic acid molecule, which is an aptamer capable of specifically adsorbing to Verotoxin-1.

Abstract: A concise method to promote growth of a plant useful for increase of food production. This invention provides a method to promote growth of a plant by administration of formic acid, an organic acid with a low molecular weight through avoidance of photo-oxidation damage. This invention also provides amino acid sequence of formate dehydrogenase and base sequence encoding the enzyme. The expression of the enzyme is induced by administration of formic acid and the enzyme is involved in metabolism, of formic acid and its salts. Furthermore, this invention provides a method to promote growth of a plant by administration of methanol through avoidance of photo-oxidation damage.

Abstract: The present invention provides an aptamer capable of specifically adsorbing to bisphenol A suspected to be an endocrine disrupter as a target molecule, a method for obtaining an aptamer capable of specifically adsorbing to bisphenol A by an in vitro selection method utilizing affinity chromatography using a carrier immobilizing bisphenol A, particularly, a method including use of an antagonistic elution buffer containing an amphiprotic organic solvent for elution by the affinity chromatography, and a single-strand nucleic acid molecule which is an aptamer obtained thereby.

Abstract: A compact and low-cost fluorescence reading apparatus capable of detecting multiple wavelengths is provided. The apparatus employs a semiconductor nanoparticle (201) as a fluorescent substance so as to enable a plurality of kinds of fluorescent substances to be simultaneously excited by a single excitation light source (220). Alternatively, the plurality of kinds of fluorescent substances are simultaneously excited by the application of a voltage.

Abstract: A concise method to promote growth of a plant useful for increase of food production. This invention provides a method to promote growth of a plant by administration of formic acid, an organic acid with a low molecular weight through avoidance of photo-oxidation damage. This invention also provides amino acid sequence of formate dehydrogenase and base sequence encoding the enzyme. The expression of the enzyme is induced by administration of formic acid and the enzyme is involved in metabolism of formic acid and its salts. Furthermore, this invention provides a method to promote growth of a plant by administration of methanol through avoidance of photo-oxidation damage.